Restriction map for
pFN21AE3155
##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BmyI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI MwoI NaeI
NarI NcoI NdeI NgoMIV NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 148 GTCTAC GT^[AC][GT]AC
AciI 269 CCGC CCGC(-3/-1)
AfaI 813 GTAC GT^AC
AlwI 1109 GGATC GGATC(4/5)
AvaII 108 GGTCC G^G[AT]CC
BcnI 1019 CCGGG CC^[GC]GG
BglII 675 AGATCT A^GATCT
BlpI 403 GCTGAGC GC^T[ACGT]AGC
BpmI 550 CTGGAG CTGGAG(16/14)
Bpu1102I 403 GCTGAGC GC^T[ACGT]AGC
BsgI 238 GTGCAG GTGCAG(16/14)
BsiYI 104 CCAAGGTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 104 CCAAGGTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 308 GAGAC GTCTC(1/5)
Bsp1407I 812 TGTACA T^GTACA
BsrGI 812 TGTACA T^GTACA
BssSI 325 CTCGTG CACGAG(-5/-1)
CelII 403 GCTGAGC GC^T[ACGT]AGC
EaeI 112 CGGCCA [CT]^GGCC[GA]
EarI 545 CTCTTC CTCTTC(1/4)
Ksp632I 545 CTCTTC CTCTTC(1/4)
MaeII 152 ACGT A^CGT
NciI 1019 CCGGG CC^[GC]GG
NheI 211 GCTAGC G^CTAGC
NspI 603 GCATGT [GA]CATG^[CT]
PshAI 1048 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 472 CTGCAG CTGCA^G
PvuII 893 CAGCTG CAG^CTG
RsaI 813 GTAC GT^AC
SfaNI 878 GCATC GCATC(5/9)
SspBI 812 TGTACA T^GTACA
SspI 691 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 284 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
716 CAGCCTCTG
BbsI 392 GAAGAC GAAGAC(2/6)
503 GTCTTC
BpuAI 392 GAAGAC GAAGAC(2/6)
503 GTCTTC
BstNI 388 CCAGG CC^[AT]GG
570 CCTGG
BstYI 675 AGATCT [GA]^GATC[CT]
1109 GGATCT
Cac8I 211 GCTAGC GC[ACGT]^[ACGT]GC
971 GCCAGC
DdeI 34 CTTAG C^T[ACGT]AG
404 CTGAG
DpnII 676 GATC ^GATC
1110 GATC
EcoRII 388 CCAGG ^CC[AT]GG
570 CCTGG
EcoT14I 104 CCAAGG C^C[AT][AT]GG
206 CCAAGG
HapII 111 CCGG C^CGG
1019 CCGG
HpaII 111 CCGG C^CGG
1019 CCGG
MboI 676 GATC ^GATC
1110 GATC
MflI 675 AGATCT [GA]^GATC[CT]
1109 GGATCT
MslI 249 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
810 CATGTACATG
MspA1I 269 CCGCTG C[AC]G^C[GT]G
893 CAGCTG
MspI 111 CCGG C^CGG
1019 CCGG
MvaI 388 CCAGG CC^[AT]GG
570 CCTGG
NdeII 676 GATC ^GATC
1110 GATC
Sau3AI 676 GATC ^GATC
1110 GATC
StyI 104 CCAAGG C^C[AT][AT]GG
206 CCAAGG
TaqI 582 TCGA T^CGA
654 TCGA
TthHB8I 582 TCGA T^CGA
654 TCGA
XhoII 675 AGATCT [GA]^GATC[CT]
1109 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BssKI
Cfr13I Fnu4HI HaeIII HinfI HphI
ItaI MaeI MaeIII MboII MnlI
MseI NlaIII PleI Sau96I ScrFI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI