Restriction map for
pFN21AE3040
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI ItaI KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MunI MvnI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I TseI Tsp45I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 12 GTCTAC GT^[AC][GT]AC
AcsI 190 AAATTC [GA]^AATT[CT]
AfaI 92 GTAC GT^AC
AocI 481 CCTGAGG CC^T[ACGT]AGG
ApoI 190 AAATTC [GA]^AATT[CT]
AspHI 509 GTGCTC G[AT]GC[AT]^C
AvaI 346 CTCGGG C^[CT]CG[GA]G
BalI 77 TGGCCA TGG^CCA
BbsI 327 GAAGAC GAAGAC(2/6)
BglII 210 AGATCT A^GATCT
BmyI 509 GTGCTC G[AGT]GC[ACT]^C
BpuAI 327 GAAGAC GAAGAC(2/6)
BsaI 352 GAGACC GGTCTC(1/5)
BsgI 449 GTGCAG GTGCAG(16/14)
BsiHKAI 509 GTGCTC G[AT]GC[AT]^C
BsmFI 379 GGGAC GGGAC(10/14)
BsoBI 346 CTCGGG C^[CT]CG[GA]G
Bsp1286I 509 GTGCTC G[AGT]GC[ACT]^C
BsrDI 99 CATTGC GCAATG(2/0)
BssSI 501 CACGAG CACGAG(-5/-1)
BstF5I 384 CATCC GGATG(2/0)
Bsu36I 481 CCTGAGG CC^T[ACGT]AGG
DraII 42 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 77 TGGCCA [CT]^GGCC[GA]
Eco81I 481 CCTGAGG CC^T[ACGT]AGG
EcoO109I 42 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 126 GCGGG CCCGC(4/6)
FokI 384 CATCC GGATG(9/13)
HincII 232 GTTGAC GT[CT]^[GA]AC
HindII 232 GTTGAC GT[CT]^[GA]AC
HindIII 200 AAGCTT A^AGCTT
MaeII 230 ACGT A^CGT
MluNI 77 TGGCCA TGG^CCA
MscI 77 TGGCCA TGG^CCA
MslI 427 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 124 CAGCGG C[AC]G^C[GT]G
NaeI 259 GCCGGC GCC^GGC
NgoMIV 259 GCCGGC G^CCGGC
NspI 275 ACATGC [GA]CATG^[CT]
PflMI 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 362 CTGCAG CTGCA^G
RsaI 92 GTAC GT^AC
SfcI 362 CTGCAG C^T[GA][CT]AG
Tsp509I 191 AATT ^AATT
TspRI 432 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 174 CCGC CCGC(-3/-1)
126 GCGG
AlwI 23 GGATC GGATC(4/5)
521 GGATC
Asp700I 165 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
208 GAAGATCTTC
BsiYI 299 CCATGGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
371 CCAAGCCTGGG
BslI 299 CCATGGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
371 CCAAGCCTGGG
BsmAI 352 GAGAC GTCTC(1/5)
392 GAGAC
BsrFI 259 GCCGGC [GA]^CCGG[CT]
288 GCCGGT
BsrI 489 ACTGG ACTGG(1/-1)
518 ACTGG
BssKI 264 CCTGG ^CC[ACGT]GG
376 CCTGG
BstNI 264 CCTGG CC^[AT]GG
376 CCTGG
BstYI 210 AGATCT [GA]^GATC[CT]
521 GGATCT
Cac8I 198 GCAAGC GC[ACGT]^[ACGT]GC
259 GCCGGC
Cfr10I 259 GCCGGC [GA]^CCGG[CT]
288 GCCGGT
EcoRII 264 CCTGG ^CC[AT]GG
376 CCTGG
EcoT14I 74 CCATGG C^C[AT][AT]GG
299 CCATGG
HapII 260 CCGG C^CGG
289 CCGG
HpaII 260 CCGG C^CGG
289 CCGG
MflI 210 AGATCT [GA]^GATC[CT]
521 GGATCT
MspI 260 CCGG C^CGG
289 CCGG
MvaI 264 CCTGG CC^[AT]GG
376 CCTGG
MwoI 153 GCGTTTTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
279 GCACTCAGGGC
NcoI 74 CCATGG C^CATGG
299 CCATGG
PleI 367 GACTC GAGTC(4/5)
338 GAGTC
ScrFI 264 CCTGG CC^[ACGT]GG
376 CCTGG
SmlI 54 CTCAAG C^T[CT][GA]AG
217 CTCAAG
StyI 74 CCATGG C^C[AT][AT]GG
299 CCATGG
TfiI 170 GATTC G^A[AT]TC
436 GATTC
XhoII 210 AGATCT [GA]^GATC[CT]
521 GGATCT
XmnI 165 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
208 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI AvaII BsaJI Cfr13I DdeI
DpnII DsaI HaeIII HinfI HphI
MboI MboII MnlI NdeII NlaIII
NlaIV Sau3AI Sau96I