Restriction map for
pFN21AE3206
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AvaI AviII AvrII
BamHI BbeI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 226 GGTACC G^GTACC
AflIII 328 ACGTGT A^C[GA][CT]GT
Alw44I 1025 GTGCAC G^TGCAC
ApaLI 1025 GTGCAC G^TGCAC
Asp718I 226 GGTACC G^GTACC
AspI 37 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1181 GGACC G^G[AT]CC
BalI 1394 TGGCCA TGG^CCA
BanI 226 GGTACC G^G[CT][GA]CC
BanII 1174 GAGCTC G[GA]GC[CT]^C
BbrPI 327 CACGTG CAC^GTG
BbsI 596 GTCTTC GAAGAC(2/6)
BbvCI 1158 GCTGAGG CCTCAGC(-5/-2)
BclI 27 TGATCA T^GATCA
BglII 1041 AGATCT A^GATCT
BpmI 1411 CTGGAG CTGGAG(16/14)
BpuAI 596 GTCTTC GAAGAC(2/6)
BsaWI 1257 TCCGGT [AT]^CCGG[AT]
BsmFI 1334 GTCCC GGGAC(10/14)
BsmI 372 GAATGC GAATGC(1/-1)
BspMI 550 ACCTGC ACCTGC(4/8)
BsrDI 1297 GCAATG GCAATG(2/0)
BstXI 326 CCACGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 264 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 957 GCGC GCG^C
Cfr13I 1181 GGACC G^G[ACGT]CC
DraI 1035 TTTAAA TTT^AAA
DraIII 701 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1394 TGGCCA [CT]^GGCC[GA]
EarI 1171 GAAGAG CTCTTC(1/4)
EcoRI 1208 GAATTC G^AATTC
FbaI 27 TGATCA T^GATCA
HaeIII 1395 GGCC GG^CC
HapII 1258 CCGG C^CGG
HhaI 957 GCGC GCG^C
HinP1I 957 GCGC G^CGC
HincII 43 GTCAAC GT[CT]^[GA]AC
HindII 43 GTCAAC GT[CT]^[GA]AC
HindIII 453 AAGCTT A^AGCTT
HpaII 1258 CCGG C^CGG
KpnI 226 GGTACC GGTAC^C
Ksp632I 1171 GAAGAG CTCTTC(1/4)
MluNI 1394 TGGCCA TGG^CCA
MscI 1394 TGGCCA TGG^CCA
MslI 157 CATACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1258 CCGG C^CGG
NdeI 1283 CATATG CA^TATG
NspI 430 GCATGT [GA]CATG^[CT]
PflFI 37 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 327 CACGTG CAC^GTG
PmlI 327 CACGTG CAC^GTG
SacI 1174 GAGCTC GAGCT^C
SapI 1171 GAAGAGC GCTCTTC(1/4)
Sau96I 1181 GGACC G^G[ACGT]CC
SexAI 841 ACCAGGT A^CC[AT]GGT
TaqI 609 TCGA T^CGA
Tth111I 37 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 609 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 427 GCGG CCGC(-3/-1)
1363 GCGG
AlwI 1255 GATCC GGATC(4/5)
1310 GGATC
AlwNI 259 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
575 CAGTTTCTG
BsaAI 116 TACGTG [CT]AC^GT[GA]
327 CACGTG
BseRI 471 CTCCTC GAGGAG(10/8)
1000 GAGGAG
BssKI 842 CCAGG ^CC[ACGT]GG
933 CCTGG
BstF5I 498 GGATG GGATG(2/0)
1005 GGATG
BstNI 842 CCAGG CC^[AT]GG
933 CCTGG
BstYI 1041 AGATCT [GA]^GATC[CT]
1254 AGATCC
EcoRII 842 CCAGG ^CC[AT]GG
933 CCTGG
FokI 498 GGATG GGATG(9/13)
1005 GGATG
MflI 1041 AGATCT [GA]^GATC[CT]
1254 AGATCC
MvaI 842 CCAGG CC^[AT]GG
933 CCTGG
MwoI 337 GCATCAGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
427 GCGGCATGTGC
PvuII 347 CAGCTG CAG^CTG
970 CAGCTG
ScrFI 842 CCAGG CC^[ACGT]GG
933 CCTGG
XhoII 1041 AGATCT [GA]^GATC[CT]
1254 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BbvI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
DdeI DpnII EcoT14I Fnu4HI HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
NdeII NlaIII NlaIV PleI RsaI
Sau3AI SfaNI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI