Restriction map for
pFN21AE3407
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaII KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AciI 439 CCGC CCGC(-3/-1)
AcsI 793 AAATTT [GA]^AATT[CT]
AfaI 436 GTAC GT^AC
ApoI 793 AAATTT [GA]^AATT[CT]
BamHI 840 GGATCC G^GATCC
BclI 579 TGATCA T^GATCA
BlpI 682 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 682 GCTCAGC GC^T[ACGT]AGC
BsaI 71 GAGACC GGTCTC(1/5)
BsaJI 831 CCTGGG C^C[ACGT][ACGT]GG
BsmBI 739 GAGACG CGTCTC(1/5)
BsmFI 646 GGGAC GGGAC(10/14)
CelII 682 GCTCAGC GC^T[ACGT]AGC
EarI 52 GAAGAG CTCTTC(1/4)
FbaI 579 TGATCA T^GATCA
HincII 240 GTTAAC GT[CT]^[GA]AC
HindII 240 GTTAAC GT[CT]^[GA]AC
HpaI 240 GTTAAC GTT^AAC
Ksp632I 52 GAAGAG CTCTTC(1/4)
MaeIII 563 GTGAC ^GT[ACGT]AC
MspA1I 482 CAGCTG C[AC]G^C[GT]G
PflMI 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 445 GAGTC GAGTC(4/5)
PstI 25 CTGCAG CTGCA^G
PvuII 482 CAGCTG CAG^CTG
RsaI 436 GTAC GT^AC
SfcI 25 CTGCAG C^T[GA][CT]AG
StuI 759 AGGCCT AGG^CCT
TaqI 735 TCGA T^CGA
TfiI 36 GATTC G^A[AT]TC
Tsp45I 563 GTGAC ^GT[GC]AC
TthHB8I 735 TCGA T^CGA
Van91I 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwNI 177 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
443 CAGAGTCTG
BbvI 608 GCAGC GCAGC(8/12)
24 GCTGC
BseRI 55 GAGGAG GAGGAG(10/8)
202 GAGGAG
BsmAI 71 GAGAC GTCTC(1/5)
739 GAGAC
BsrI 182 ACTGG ACTGG(1/-1)
399 CCAGT
BstF5I 490 CATCC GGATG(2/0)
453 GGATG
DraI 94 TTTAAA TTT^AAA
598 TTTAAA
Fnu4HI 24 GCTGC GC^[ACGT]GC
608 GCAGC
FokI 490 CATCC GGATG(9/13)
453 GGATG
HaeIII 760 GGCC GG^CC
835 GGCC
HinfI 36 GATTC G^A[ACGT]TC
445 GAGTC
HphI 744 GGTGA GGTGA(8/7)
41 TCACC
ItaI 24 GCTGC GC^[ACGT]GC
608 GCAGC
NlaIV 646 GGGACC GG[ACGT]^[ACGT]CC
840 GGATCC
SfaNI 386 GATGC GCATC(5/9)
501 GATGC
TseI 24 GCTGC G^C[AT]GC
608 GCAGC
TspRI 181 CACTG CA[GC]TG[ACGT][ACGT]^
400 CAGTG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
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AluI AlwI AvaII BsiYI BslI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII MboI MboII
MflI MnlI MseI MvaI NdeII
NlaIII Sau3AI Sau96I ScrFI Tru9I
Tsp509I XhoII