################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AocI ApoI AscI AseI AsnI Asp700I Asp718I AvaI AvrII BalI BbeI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsoBI BspDI BspEI BspHI BsrDI Bst1107I BstZ17I Bsu36I CelII ClaI CpoI DraI EarI Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluNI MroI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 1080 GTCTAC GT^[AC][GT]AC AcyI 223 GGCGTC G[GA]^CG[CT]C AhdI 112 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1240 AGCGCT AGC^GCT ApaI 211 GGGCCC GGGCC^C AspEI 112 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 220 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1613 TGCGCA TGC^GCA BamHI 1408 GGATCC G^GATCC BanI 1560 GGTGCC G^G[CT][GA]CC BbrPI 802 CACGTG CAC^GTG BciVI 535 GGATAC GTATCC(6/5) BsaAI 802 CACGTG [CT]AC^GT[GA] BsaHI 223 GGCGTC G[GA]^CG[CT]C BsaI 316 GAGACC GGTCTC(1/5) BseRI 1250 CTCCTC GAGGAG(10/8) BsiEI 1619 CGGCCG CG[GA][CT]^CG BsrBI 951 CCGCTC CCGCTC(-3/-3) BssHII 156 GCGCGC G^CGCGC BstBI 485 TTCGAA TT^CGAA BstYI 1408 GGATCC [GA]^GATC[CT] DraIII 653 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1619 CGGCCG C^GGCCG Eam1105I 112 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1619 CGGCCG C^GGCCG Eco47III 1240 AGCGCT AGC^GCT Eco52I 1619 CGGCCG C^GGCCG EcoNI 1029 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1613 TGCGCA TGC^GCA HaeII 1240 AGCGCT [GA]GCGC^[CT] Hin1I 223 GGCGTC G[GA]^CG[CT]C MflI 1408 GGATCC [GA]^GATC[CT] MluI 185 ACGCGT A^CGCGT NaeI 1383 GCCGGC GCC^GGC NcoI 1638 CCATGG C^CATGG NgoMIV 1383 GCCGGC G^CCGGC NspV 485 TTCGAA TT^CGAA PflFI 220 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 615 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 802 CACGTG CAC^GTG PmlI 802 CACGTG CAC^GTG PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT] PshAI 408 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 352 GAGCTC GAGCT^C ScaI 878 AGTACT AGT^ACT SfuI 485 TTCGAA TT^CGAA SgrAI 1532 CACCGGCG C[GA]^CCGG[CT]G TfiI 736 GATTC G^A[AT]TC Tth111I 220 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 615 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1408 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 805 GTGCAC G^TGCAC 940 GTGCAC ApaLI 805 GTGCAC G^TGCAC 940 GTGCAC AvaII 774 GGACC G^G[AT]CC 1070 GGACC BanII 211 GGGCCC G[GA]GC[CT]^C 352 GAGCTC BsgI 436 CTGCAC GTGCAG(16/14) 919 GTGCAG BsmBI 1505 CGTCTC CGTCTC(1/5) 182 GAGACG Bsp1407I 680 TGTACA T^GTACA 1292 TGTACA BspLU11I 590 ACATGT A^CATGT 689 ACATGT BsrGI 680 TGTACA T^GTACA 1292 TGTACA BssSI 138 CACGAG CACGAG(-5/-1) 1591 CTCGTG BstEII 744 GGTGACC G^GT[ACGT]ACC 867 GGTCACC BstPI 744 GGTGACC G^GT[ACGT]ACC 867 GGTCACC DraII 211 GGGCCCC [GA]G^G[ACGT]CC[CT] 773 AGGACCT DrdI 1071 GACCGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1348 GACTCTCACGTC EcoO109I 211 GGGCCCC [GA]G^G[ACGT]CC[CT] 773 AGGACCT EcoO65I 744 GGTGACC G^GT[ACGT]ACC 867 GGTCACC NspI 590 ACATGT [GA]CATG^[CT] 689 ACATGT PvuII 106 CAGCTG CAG^CTG 1021 CAGCTG SspBI 680 TGTACA T^GTACA 1292 TGTACA TaqI 486 TCGA T^CGA 782 TCGA Tsp509I 985 AATT ^AATT 1103 AATT TthHB8I 486 TCGA T^CGA 782 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI