Restriction map for
pFN21AE3524
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AvaI
AvrII BalI BbeI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsoBI BspDI BspEI BspHI
BsrDI Bst1107I BstZ17I Bsu36I CelII
ClaI CpoI DraI EarI Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluNI MroI MscI MseI MunI
NarI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1080 GTCTAC GT^[AC][GT]AC
AcyI 223 GGCGTC G[GA]^CG[CT]C
AhdI 112 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1240 AGCGCT AGC^GCT
ApaI 211 GGGCCC GGGCC^C
AspEI 112 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 220 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1613 TGCGCA TGC^GCA
BamHI 1408 GGATCC G^GATCC
BanI 1560 GGTGCC G^G[CT][GA]CC
BbrPI 802 CACGTG CAC^GTG
BciVI 535 GGATAC GTATCC(6/5)
BsaAI 802 CACGTG [CT]AC^GT[GA]
BsaHI 223 GGCGTC G[GA]^CG[CT]C
BsaI 316 GAGACC GGTCTC(1/5)
BseRI 1250 CTCCTC GAGGAG(10/8)
BsiEI 1619 CGGCCG CG[GA][CT]^CG
BsrBI 951 CCGCTC CCGCTC(-3/-3)
BssHII 156 GCGCGC G^CGCGC
BstBI 485 TTCGAA TT^CGAA
BstYI 1408 GGATCC [GA]^GATC[CT]
DraIII 653 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1619 CGGCCG C^GGCCG
Eam1105I 112 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1619 CGGCCG C^GGCCG
Eco47III 1240 AGCGCT AGC^GCT
Eco52I 1619 CGGCCG C^GGCCG
EcoNI 1029 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1613 TGCGCA TGC^GCA
HaeII 1240 AGCGCT [GA]GCGC^[CT]
Hin1I 223 GGCGTC G[GA]^CG[CT]C
MflI 1408 GGATCC [GA]^GATC[CT]
MluI 185 ACGCGT A^CGCGT
NaeI 1383 GCCGGC GCC^GGC
NcoI 1638 CCATGG C^CATGG
NgoMIV 1383 GCCGGC G^CCGGC
NspV 485 TTCGAA TT^CGAA
PflFI 220 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 615 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 802 CACGTG CAC^GTG
PmlI 802 CACGTG CAC^GTG
PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 408 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 352 GAGCTC GAGCT^C
ScaI 878 AGTACT AGT^ACT
SfuI 485 TTCGAA TT^CGAA
SgrAI 1532 CACCGGCG C[GA]^CCGG[CT]G
TfiI 736 GATTC G^A[AT]TC
Tth111I 220 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 615 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1408 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 805 GTGCAC G^TGCAC
940 GTGCAC
ApaLI 805 GTGCAC G^TGCAC
940 GTGCAC
AvaII 774 GGACC G^G[AT]CC
1070 GGACC
BanII 211 GGGCCC G[GA]GC[CT]^C
352 GAGCTC
BsgI 436 CTGCAC GTGCAG(16/14)
919 GTGCAG
BsmBI 1505 CGTCTC CGTCTC(1/5)
182 GAGACG
Bsp1407I 680 TGTACA T^GTACA
1292 TGTACA
BspLU11I 590 ACATGT A^CATGT
689 ACATGT
BsrGI 680 TGTACA T^GTACA
1292 TGTACA
BssSI 138 CACGAG CACGAG(-5/-1)
1591 CTCGTG
BstEII 744 GGTGACC G^GT[ACGT]ACC
867 GGTCACC
BstPI 744 GGTGACC G^GT[ACGT]ACC
867 GGTCACC
DraII 211 GGGCCCC [GA]G^G[ACGT]CC[CT]
773 AGGACCT
DrdI 1071 GACCGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1348 GACTCTCACGTC
EcoO109I 211 GGGCCCC [GA]G^G[ACGT]CC[CT]
773 AGGACCT
EcoO65I 744 GGTGACC G^GT[ACGT]ACC
867 GGTCACC
NspI 590 ACATGT [GA]CATG^[CT]
689 ACATGT
PvuII 106 CAGCTG CAG^CTG
1021 CAGCTG
SspBI 680 TGTACA T^GTACA
1292 TGTACA
TaqI 486 TCGA T^CGA
782 TCGA
Tsp509I 985 AATT ^AATT
1103 AATT
TthHB8I 486 TCGA T^CGA
782 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI