Restriction map for
pFN21AE3527
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvaII AviII AvrII BbrPI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MroI MunI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SfcI
SfiI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 511 ACGCGT A^C[GA][CT]GT
AlwNI 122 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 255 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1023 GGATCC G^GATCC
BbvCI 1273 CCTCAGC CCTCAGC(-5/-2)
BfaI 656 CTAG C^TAG
BglI 472 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1016 AGATCT A^GATCT
BsmBI 835 GAGACG CGTCTC(1/5)
BsmI 970 GAATGC GAATGC(1/-1)
BspMI 1037 ACCTGC ACCTGC(4/8)
BssSI 214 CTCGTG CACGAG(-5/-1)
BstBI 1061 TTCGAA TT^CGAA
BstF5I 27 GGATG GGATG(2/0)
BstXI 443 CCAGCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 761 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 660 CTCTTC CTCTTC(1/4)
EcoO109I 761 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 27 GGATG GGATG(9/13)
HgaI 510 GACGC GACGC(5/10)
Ksp632I 660 CTCTTC CTCTTC(1/4)
MaeI 656 CTAG C^TAG
MaeII 616 ACGT A^CGT
MaeIII 730 GTCAC ^GT[ACGT]AC
MluI 511 ACGCGT A^CGCGT
MspA1I 774 CCGCTG C[AC]G^C[GT]G
NaeI 1113 GCCGGC GCC^GGC
NcoI 449 CCATGG C^CATGG
NgoMIV 1113 GCCGGC G^CCGGC
NspI 68 GCATGT [GA]CATG^[CT]
NspV 1061 TTCGAA TT^CGAA
PflFI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 659 GCTCTTC GCTCTTC(1/4)
SexAI 296 ACCTGGT A^CC[AT]GGT
SfuI 1061 TTCGAA TT^CGAA
Tsp45I 730 GTCAC ^GT[GC]AC
Tsp509I 1103 AATT ^AATT
Tth111I 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 255 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 339 GTAC GT^AC
594 GTAC
BanII 421 GGGCTC G[GA]GC[CT]^C
712 GGGCTC
BbsI 507 GAAGAC GAAGAC(2/6)
1088 GAAGAC
BpuAI 507 GAAGAC GAAGAC(2/6)
1088 GAAGAC
BsaI 7 GAGACC GGTCTC(1/5)
884 GGTCTC
BseRI 426 CTCCTC GAGGAG(10/8)
1223 CTCCTC
BsiEI 323 CGGTCG CG[GA][CT]^CG
830 CGACCG
PflMI 121 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1014 CCAGATCTTGG
PleI 1091 GACTC GAGTC(4/5)
1159 GAGTC
RsaI 339 GTAC GT^AC
594 GTAC
SmaI 11 CCCGGG CCC^GGG
610 CCCGGG
TfiI 1143 GATTC G^A[AT]TC
1263 GATTC
Van91I 121 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1014 CCAGATCTTGG
XcmI 755 CCATTAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
842 CCATTCAGCTTATGG
XmaCI 11 CCCGGG C^CCGGG
610 CCCGGG
XmaI 11 CCCGGG C^CCGGG
610 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI BalI BanI BbeI
BbvI BcgI BcnI BmyI BpmI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfoI StyI TaqI Tru9I
TseI TspRI TthHB8I XhoII