##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        Fnu4HI      
FseI        FspI        HaeII       Hin1I       HpaI        
ItaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacII       SalI        
SbfI        ScaI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
TseI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI         1303  GTCTAC               GT^[AC][GT]AC
AocI         1309  CCTAAGG              CC^T[ACGT]AGG
BbsI         1233  GAAGAC               GAAGAC(2/6)
BpmI          638  CTGGAG               CTGGAG(16/14)
BpuAI        1233  GAAGAC               GAAGAC(2/6)
BsgI         1497  GTGCAG               GTGCAG(16/14)
Bsp1407I     1371  TGTACA               T^GTACA
BspHI          83  TCATGA               T^CATGA
BsrGI        1371  TGTACA               T^GTACA
BstXI         903  CCATAAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1309  CCTAAGG              CC^T[ACGT]AGG
CfoI         1698  GCGC                 GCG^C
DraII         562  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        194  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1309  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      562  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       313  CCAAGG               C^C[AT][AT]GG
FauI           18  CCCGC                CCCGC(4/6)
HgaI         1762  GACGC                GACGC(5/10)
HhaI         1698  GCGC                 GCG^C
HinP1I       1698  GCGC                 G^CGC
MwoI          864  GCTTGTTGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI           25  GAGTC                GAGTC(4/5)
RcaI           83  TCATGA               T^CATGA
SapI          840  GAAGAGC              GCTCTTC(1/4)
SexAI         104  ACCTGGT              A^CC[AT]GGT
SspBI        1371  TGTACA               T^GTACA
StuI         1598  AGGCCT               AGG^CCT
StyI          313  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI           19  CCGC                 CCGC(-3/-1)
              432  CCGC                
AvaI            9  CCCGAG               C^[CT]CG[GA]G
             1614  CTCGGG              
AvaII          51  GGACC                G^G[AT]CC
              462  GGTCC               
BamHI         237  GGATCC               G^GATCC
             1049  GGATCC              
BsaI         1485  GAGACC               GGTCTC(1/5)
              108  GGTCTC              
BseRI          34  GAGGAG               GAGGAG(10/8)
              142  GAGGAG              
BsoBI           9  CCCGAG               C^[CT]CG[GA]G
             1614  CTCGGG              
BsrDI         860  CATTGC               GCAATG(2/0)
              491  GCAATG              
EarI          388  GAAGAG               CTCTTC(1/4)
              840  GAAGAG              
HincII       1691  GTCAAC               GT[CT]^[GA]AC
             1708  GTCAAC              
HindII       1691  GTCAAC               GT[CT]^[GA]AC
             1708  GTCAAC              
HindIII      1431  AAGCTT               A^AGCTT
             1515  AAGCTT              
Ksp632I       388  GAAGAG               CTCTTC(1/4)
              840  GAAGAG              
Tsp45I        746  GTGAC                ^GT[GC]AC
             1760  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AspHI       BanII       BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       EcoRI       EcoRII      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
NdeII       NlaIII      NlaIV       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       Tsp509I     TspRI       TthHB8I     
XhoII       XmnI