Restriction map for
pFN21AE9575
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbsI BbvCI BciVI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI Eam1105I Eco81I EcoNI
EcoRV EcoT14I EcoT22I FseI HincII
HindII HindIII HinfI HpaI KpnI
MaeI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI TfiI
Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AatII 529 GACGTC GACGT^C
AcsI 895 GAATTC [GA]^AATT[CT]
Alw44I 499 GTGCAC G^TGCAC
AlwI 164 GATCC GGATC(4/5)
AlwNI 43 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 157 GGGCCC GGGCC^C
ApaLI 499 GTGCAC G^TGCAC
ApoI 895 GAATTC [GA]^AATT[CT]
AspI 987 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 157 GGGCCC G[GA]GC[CT]^C
BbeI 401 GGCGCC GGCGC^C
BbrPI 817 CACGTG CAC^GTG
BcgI 1005 CGAGGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 562 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 384 GCTCAGC GC^T[ACGT]AGC
BpmI 1054 CTCCAG CTGGAG(16/14)
Bpu1102I 384 GCTCAGC GC^T[ACGT]AGC
BsaAI 817 CACGTG [CT]AC^GT[GA]
BseRI 670 GAGGAG GAGGAG(10/8)
BsgI 431 GTGCAG GTGCAG(16/14)
BsiEI 681 CGGCCG CG[GA][CT]^CG
Bsp1407I 821 TGTACA T^GTACA
BspHI 182 TCATGA T^CATGA
BspLU11I 317 ACATGT A^CATGT
BsrBI 382 CCGCTC CCGCTC(-3/-3)
BsrDI 247 CATTGC GCAATG(2/0)
BsrGI 821 TGTACA T^GTACA
BsrI 263 ACTGG ACTGG(1/-1)
BstAPI 42 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 294 GGTGACC G^GT[ACGT]ACC
BstPI 294 GGTGACC G^GT[ACGT]ACC
BstYI 163 AGATCC [GA]^GATC[CT]
CelII 384 GCTCAGC GC^T[ACGT]AGC
DrdI 456 GACCGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 681 CGGCCG C^GGCCG
EclXI 681 CGGCCG C^GGCCG
Eco52I 681 CGGCCG C^GGCCG
EcoO65I 294 GGTGACC G^GT[ACGT]ACC
EcoRI 895 GAATTC G^AATTC
KasI 401 GGCGCC G^GCGCC
KspI 716 CCGCGG CCGC^GG
MflI 163 AGATCC [GA]^GATC[CT]
NaeI 146 GCCGGC GCC^GGC
NarI 401 GGCGCC GG^CGCC
NgoMIV 146 GCCGGC G^CCGGC
NspI 317 ACATGT [GA]CATG^[CT]
PflFI 987 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 392 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 817 CACGTG CAC^GTG
PmlI 817 CACGTG CAC^GTG
PstI 40 CTGCAG CTGCA^G
RcaI 182 TCATGA T^CATGA
SacII 716 CCGCGG CCGC^GG
SfcI 40 CTGCAG C^T[GA][CT]AG
SfiI 561 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 401 GGCGCC GGC^GCC
SspBI 821 TGTACA T^GTACA
SspI 917 AATATT AAT^ATT
TaqI 527 TCGA T^CGA
Tsp509I 896 AATT ^AATT
TspRI 262 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 987 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 527 TCGA T^CGA
Van91I 392 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 941 CCAGAGACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 163 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 401 GGCGCC G[GA]^CG[CT]C
529 GACGTC
AflIII 317 ACATGT A^C[GA][CT]GT
818 ACGTGT
Aor51HI 359 AGCGCT AGC^GCT
395 AGCGCT
AspHI 499 GTGCAC G[AT]GC[AT]^C
766 GTGCTC
AvaI 114 CCCGGG C^[CT]CG[GA]G
890 CCCGGG
AviII 662 TGCGCA TGC^GCA
1014 TGCGCA
BalI 215 TGGCCA TGG^CCA
560 TGGCCA
BanI 401 GGCGCC G^G[CT][GA]CC
936 GGTGCC
BclI 185 TGATCA T^GATCA
620 TGATCA
BsaHI 401 GGCGCC G[GA]^CG[CT]C
529 GACGTC
BsiHKAI 499 GTGCAC G[AT]GC[AT]^C
766 GTGCTC
BsmAI 944 GAGAC GTCTC(1/5)
839 GTCTC
BsoBI 114 CCCGGG C^[CT]CG[GA]G
890 CCCGGG
BssHII 475 GCGCGC G^CGCGC
699 GCGCGC
DraIII 778 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
831 CACGCTGTG
Eco47III 359 AGCGCT AGC^GCT
395 AGCGCT
FbaI 185 TGATCA T^GATCA
620 TGATCA
FspI 662 TGCGCA TGC^GCA
1014 TGCGCA
Hin1I 401 GGCGCC G[GA]^CG[CT]C
529 GACGTC
MluNI 215 TGGCCA TGG^CCA
560 TGGCCA
MscI 215 TGGCCA TGG^CCA
560 TGGCCA
MslI 177 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
990 CACGTCCGTG
PvuII 843 CAGCTG CAG^CTG
901 CAGCTG
SmaI 114 CCCGGG CCC^GGG
890 CCCGGG
SmlI 535 CTCAAG C^T[CT][GA]AG
652 CTCAAG
XmaCI 114 CCCGGG C^CCGGG
890 CCCGGG
XmaI 114 CCCGGG C^CCGGG
890 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BspMI BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I