Restriction map for
pFN21AE3507
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# NO CUT ENZYMES ( 80 enzymes) #
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Acc65I AccI AclI AflII Aor51HI
AscI AseI AsnI Asp718I AspI
AvrII BbvCI BciVI BclI BfrI
BglII BlnI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KpnI MfeI MluI
MseI MunI NaeI NgoMIV NheI
NotI NsiI NspV PacI PflFI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 1701 GACGTC GACGT^C
AccIII 248 TCCGGA T^CCGGA
AflIII 311 ACGTGT A^C[GA][CT]GT
AgeI 1322 ACCGGT A^CCGGT
BbrPI 1222 CACGTG CAC^GTG
BfaI 2053 CTAG C^TAG
BlpI 1270 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1270 GCTGAGC GC^T[ACGT]AGC
BsaAI 1222 CACGTG [CT]AC^GT[GA]
BsaBI 2241 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 248 TCCGGA T^CCGGA
BspEI 248 TCCGGA T^CCGGA
BstXI 1493 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1270 GCTGAGC GC^T[ACGT]AGC
HindIII 1816 AAGCTT A^AGCTT
MaeI 2053 CTAG C^TAG
MamI 2241 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 248 TCCGGA T^CCGGA
NcoI 1862 CCATGG C^CATGG
NdeI 281 CATATG CA^TATG
NruI 1755 TCGCGA TCG^CGA
PinAI 1322 ACCGGT A^CCGGT
PmaCI 1222 CACGTG CAC^GTG
PmlI 1222 CACGTG CAC^GTG
PshAI 82 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2056 GAGCTC GAGCT^C
ScaI 344 AGTACT AGT^ACT
SmaI 135 CCCGGG CCC^GGG
SphI 1958 GCATGC GCATG^C
StuI 2006 AGGCCT AGG^CCT
XmaCI 135 CCCGGG C^CCGGG
XmaI 135 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 40 enzymes) #
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AcsI 447 AAATTT [GA]^AATT[CT]
1420 AAATTC
AhdI 40 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1746 GACGCAGTGTC
Alw44I 412 GTGCAC G^TGCAC
1030 GTGCAC
AocI 826 CCTGAGG CC^T[ACGT]AGG
1294 CCTGAGG
ApaI 218 GGGCCC GGGCC^C
997 GGGCCC
ApaLI 412 GTGCAC G^TGCAC
1030 GTGCAC
ApoI 447 AAATTT [GA]^AATT[CT]
1420 AAATTC
Asp700I 567 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2403 GAACGACTTC
AspEI 40 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1746 GACGCAGTGTC
BalI 611 TGGCCA TGG^CCA
1502 TGGCCA
BbeI 105 GGCGCC GGCGC^C
1312 GGCGCC
BcgI 382 CGAAGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1832 GCATCCGCATCG
BglI 539 GCCAGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1653 GCCCCAGCGGC
BsaWI 248 TCCGGA [AT]^CCGG[AT]
1322 ACCGGT
BsiEI 1320 CGACCG CG[GA][CT]^CG
2190 CGACCG
BsmBI 2459 GAGACG CGTCTC(1/5)
2776 GAGACG
BspHI 878 TCATGA T^CATGA
2654 TCATGA
BssSI 1393 CACGAG CACGAG(-5/-1)
624 CTCGTG
Bsu36I 826 CCTGAGG CC^T[ACGT]AGG
1294 CCTGAGG
DrdI 306 GACCAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1369 GACGCCCACGTC
Eam1105I 40 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1746 GACGCAGTGTC
Eco81I 826 CCTGAGG CC^T[ACGT]AGG
1294 CCTGAGG
EcoNI 797 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
984 CCTGAGCCAGG
KasI 105 GGCGCC G^GCGCC
1312 GGCGCC
KspI 7 CCGCGG CCGC^GG
109 CCGCGG
MluNI 611 TGGCCA TGG^CCA
1502 TGGCCA
MscI 611 TGGCCA TGG^CCA
1502 TGGCCA
NarI 105 GGCGCC GG^CGCC
1312 GGCGCC
NspI 1958 GCATGC [GA]CATG^[CT]
2122 ACATGC
PaeR7I 1400 CTCGAG C^TCGAG
1678 CTCGAG
PflMI 186 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1613 CCATGCCCTGG
RcaI 878 TCATGA T^CATGA
2654 TCATGA
SacII 7 CCGCGG CCGC^GG
109 CCGCGG
SapI 2377 GAAGAGC GCTCTTC(1/4)
147 GCTCTTC
SfoI 105 GGCGCC GGC^GCC
1312 GGCGCC
TfiI 475 GAATC G^A[AT]TC
572 GATTC
Tsp509I 448 AATT ^AATT
1421 AATT
Van91I 186 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1613 CCATGCCCTGG
XhoI 1400 CTCGAG C^TCGAG
1678 CTCGAG
XmnI 567 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2403 GAACGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
AviII BamHI BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII