Restriction map for
pFN21AE3509
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BlnI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KspI MfeI MluI MroI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 640 GGTACC G^GTACC
AclI 1590 AACGTT AA^CGTT
AcsI 1120 GAATTT [GA]^AATT[CT]
AlwNI 1727 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 870 CCTGAGG CC^T[ACGT]AGG
ApaI 1720 GGGCCC GGGCC^C
ApoI 1120 GAATTT [GA]^AATT[CT]
Asp700I 1656 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 640 GGTACC G^GTACC
AspI 254 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 611 TGGCCA TGG^CCA
BanI 640 GGTACC G^G[CT][GA]CC
BbvCI 1566 CCTCAGC CCTCAGC(-5/-2)
BglI 1325 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 803 AGATCT A^GATCT
BlpI 1263 GCTGAGC GC^T[ACGT]AGC
BpmI 284 CTGGAG CTGGAG(16/14)
Bpu1102I 1263 GCTGAGC GC^T[ACGT]AGC
BsaAI 451 TACGTG [CT]AC^GT[GA]
BsaBI 108 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 237 GAGACG CGTCTC(1/5)
BstAPI 741 GCAGATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
CelII 1263 GCTGAGC GC^T[ACGT]AGC
DrdI 239 GACGCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 399 CCACGG C^C[GA][CT]GG
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
HgaI 239 GACGC GACGC(5/10)
KpnI 640 GGTACC GGTAC^C
MamI 108 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 611 TGGCCA TGG^CCA
MscI 611 TGGCCA TGG^CCA
NheI 393 GCTAGC G^CTAGC
NruI 996 TCGCGA TCG^CGA
NspI 1682 ACATGC [GA]CATG^[CT]
PflFI 254 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1251 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1590 AACGTT AA^CGTT
PvuII 862 CAGCTG CAG^CTG
SacI 1272 GAGCTC GAGCT^C
SmaI 1534 CCCGGG CCC^GGG
StuI 1550 AGGCCT AGG^CCT
TfiI 1130 GAATC G^A[AT]TC
Tsp509I 1121 AATT ^AATT
Tth111I 254 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1251 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1478 CCAGCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1534 CCCGGG C^CCGGG
XmaI 1534 CCCGGG C^CCGGG
XmnI 1656 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 641 GTAC GT^AC
1563 GTAC
AvaI 617 CTCGGG C^[CT]CG[GA]G
1534 CCCGGG
BbsI 436 GTCTTC GAAGAC(2/6)
703 GTCTTC
BpuAI 436 GTCTTC GAAGAC(2/6)
703 GTCTTC
BsoBI 617 CTCGGG C^[CT]CG[GA]G
1534 CCCGGG
BsrI 117 CCAGT ACTGG(1/-1)
163 CCAGT
BstXI 342 CCAGGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1478 CCAGCAGGGTGG
CfoI 264 GCGC GCG^C
903 GCGC
DraIII 208 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1230 CACCCTGTG
EcoNI 786 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
870 CCTGAGGAAGG
HhaI 264 GCGC GCG^C
903 GCGC
HinP1I 264 GCGC G^CGC
903 GCGC
RsaI 641 GTAC GT^AC
1563 GTAC
TspRI 508 CAGTG CA[GC]TG[ACGT][ACGT]^
1061 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BssKI BstF5I BstNI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I XhoII