Restriction map for
pFN21AE3530
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII Alw44I ApaLI ApoI AscI
AseI AsnI Asp718I BamHI BcgI
BclI BfrI BglII BsiWI BsmI
BspDI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AgeI 353 ACCGGT A^CCGGT
AhdI 1327 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3637 AGCGCT AGC^GCT
Asp700I 1125 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1327 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 498 CCTAGG C^CTAGG
BbrPI 3361 CACGTG CAC^GTG
BciVI 893 GGATAC GTATCC(6/5)
BlnI 498 CCTAGG C^CTAGG
BlpI 942 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 942 GCTGAGC GC^T[ACGT]AGC
BsiEI 2058 CGGCCG CG[GA][CT]^CG
Bsp1407I 1109 TGTACA T^GTACA
BspHI 620 TCATGA T^CATGA
BsrBI 636 GAGCGG CCGCTC(-3/-3)
BsrDI 275 GCAATG GCAATG(2/0)
BsrGI 1109 TGTACA T^GTACA
BssSI 3340 CACGAG CACGAG(-5/-1)
BstEII 1528 GGTGACC G^GT[ACGT]ACC
BstPI 1528 GGTGACC G^GT[ACGT]ACC
CelII 942 GCTGAGC GC^T[ACGT]AGC
EagI 2058 CGGCCG C^GGCCG
Eam1105I 1327 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2058 CGGCCG C^GGCCG
Eco47III 3637 AGCGCT AGC^GCT
Eco52I 2058 CGGCCG C^GGCCG
EcoO65I 1528 GGTGACC G^GT[ACGT]ACC
HindIII 2895 AAGCTT A^AGCTT
KspI 1533 CCGCGG CCGC^GG
MseI 1547 TTAA T^TAA
NcoI 1094 CCATGG C^CATGG
PaeR7I 1186 CTCGAG C^TCGAG
PflMI 2622 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 353 ACCGGT A^CCGGT
PmaCI 3361 CACGTG CAC^GTG
PmlI 3361 CACGTG CAC^GTG
RcaI 620 TCATGA T^CATGA
SacI 507 GAGCTC GAGCT^C
SacII 1533 CCGCGG CCGC^GG
SapI 2199 GAAGAGC GCTCTTC(1/4)
SbfI 2703 CCTGCAGG CCTGCA^GG
SexAI 1406 ACCAGGT A^CC[AT]GGT
Sse8387I 2703 CCTGCAGG CCTGCA^GG
SspBI 1109 TGTACA T^GTACA
TfiI 1045 GATTC G^A[AT]TC
Tru9I 1547 TTAA T^TAA
Tsp509I 1545 AATT ^AATT
Van91I 2622 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1186 CTCGAG C^TCGAG
XmnI 1125 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 830 TCCGGA T^CCGGA
1323 TCCGGA
AocI 1475 CCTCAGG CC^T[ACGT]AGG
1793 CCTCAGG
AspI 394 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1222 GACCGTGTC
BalI 2138 TGGCCA TGG^CCA
3596 TGGCCA
BbeI 1774 GGCGCC GGCGC^C
3462 GGCGCC
BbsI 660 GAAGAC GAAGAC(2/6)
1567 GTCTTC
BfaI 499 CTAG C^TAG
1102 CTAG
BpuAI 660 GAAGAC GAAGAC(2/6)
1567 GTCTTC
BsaBI 418 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3030 GATCAACATC
BseAI 830 TCCGGA T^CCGGA
1323 TCCGGA
BspEI 830 TCCGGA T^CCGGA
1323 TCCGGA
Bsu36I 1475 CCTCAGG CC^T[ACGT]AGG
1793 CCTCAGG
Eco81I 1475 CCTCAGG CC^T[ACGT]AGG
1793 CCTCAGG
KasI 1774 GGCGCC G^GCGCC
3462 GGCGCC
MaeI 499 CTAG C^TAG
1102 CTAG
MamI 418 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3030 GATCAACATC
MluNI 2138 TGGCCA TGG^CCA
3596 TGGCCA
MroI 830 TCCGGA T^CCGGA
1323 TCCGGA
MscI 2138 TGGCCA TGG^CCA
3596 TGGCCA
NaeI 3459 GCCGGC GCC^GGC
3551 GCCGGC
NarI 1774 GGCGCC GG^CGCC
3462 GGCGCC
NgoMIV 3459 GCCGGC G^CCGGC
3551 GCCGGC
NspI 1295 ACATGC [GA]CATG^[CT]
1583 ACATGC
PflFI 394 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1222 GACCGTGTC
SfoI 1774 GGCGCC GGC^GCC
3462 GGCGCC
StuI 1160 AGGCCT AGG^CCT
1784 AGGCCT
Tth111I 394 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1222 GACCGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII AviII BanI BanII
BbvCI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI