################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbeI BbsI BcgI BclI BfrI BglII BlnI BpuAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I EarI EcoRI EcoRV EcoT22I FbaI FseI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MroI MseI MunI NarI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 188 GGTACC G^GTACC AflIII 1571 ACATGT A^C[GA][CT]GT AocI 569 CCTCAGG CC^T[ACGT]AGG Aor51HI 1515 AGCGCT AGC^GCT Asp718I 188 GGTACC G^GTACC AviII 1253 TGCGCA TGC^GCA BalI 1005 TGGCCA TGG^CCA BbvCI 711 CCTCAGC CCTCAGC(-5/-2) BciVI 1337 GGATAC GTATCC(6/5) BlpI 1740 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1740 GCTGAGC GC^T[ACGT]AGC BspLU11I 1571 ACATGT A^CATGT BsrDI 836 CATTGC GCAATG(2/0) BssSI 415 CTCGTG CACGAG(-5/-1) BstAPI 835 GCATTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 569 CCTCAGG CC^T[ACGT]AGG CelII 1740 GCTGAGC GC^T[ACGT]AGC DraIII 1840 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1515 AGCGCT AGC^GCT Eco81I 569 CCTCAGG CC^T[ACGT]AGG FspI 1253 TGCGCA TGC^GCA KpnI 188 GGTACC GGTAC^C MluNI 1005 TGGCCA TGG^CCA MscI 1005 TGGCCA TGG^CCA NaeI 1415 GCCGGC GCC^GGC NgoMIV 1415 GCCGGC G^CCGGC NheI 553 GCTAGC G^CTAGC PaeR7I 511 CTCGAG C^TCGAG Tsp509I 137 AATT ^AATT XcmI 518 CCAGCTATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 511 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 27 CGCG CG^CG 1584 CGCG AspI 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2050 GACAAGGTC BbrPI 291 CACGTG CAC^GTG 403 CACGTG BglI 566 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 575 GCCCCAAGGGC BsaAI 291 CACGTG [CT]AC^GT[GA] 403 CACGTG BsiEI 1544 CGGCCG CG[GA][CT]^CG 1948 CGGCCG BsrBI 2094 CCGCTC CCGCTC(-3/-3) 1507 GAGCGG BstEII 639 GGTCACC G^GT[ACGT]ACC 1641 GGTGACC BstPI 639 GGTCACC G^GT[ACGT]ACC 1641 GGTGACC BstUI 27 CGCG CG^CG 1584 CGCG BstYI 168 GGATCT [GA]^GATC[CT] 725 AGATCC EagI 1544 CGGCCG C^GGCCG 1948 CGGCCG EclXI 1544 CGGCCG C^GGCCG 1948 CGGCCG Eco52I 1544 CGGCCG C^GGCCG 1948 CGGCCG EcoO65I 639 GGTCACC G^GT[ACGT]ACC 1641 GGTGACC HaeII 1515 AGCGCT [GA]GCGC^[CT] 2033 GGCGCT HgaI 1293 GACGC GACGC(5/10) 605 GCGTC MboII 1686 GAAGA GAAGA(8/7) 1180 TCTTC MflI 168 GGATCT [GA]^GATC[CT] 725 AGATCC MslI 1311 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1909 CACACCAATG MvnI 27 CGCG CG^CG 1584 CGCG NspI 1305 GCATGT [GA]CATG^[CT] 1571 ACATGT PflFI 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2050 GACAAGGTC PflMI 58 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2073 CCACAAGCTGG PmaCI 291 CACGTG CAC^GTG 403 CACGTG PmlI 291 CACGTG CAC^GTG 403 CACGTG PpuMI 34 GGGTCCC [GA]G^G[AT]CC[CT] 2054 AGGTCCT PstI 815 CTGCAG CTGCA^G 1930 CTGCAG SacI 148 GAGCTC GAGCT^C 1954 GAGCTC TaqI 512 TCGA T^CGA 2048 TCGA Tth111I 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2050 GACAAGGTC TthHB8I 512 TCGA T^CGA 2048 TCGA Van91I 58 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2073 CCACAAGCTGG XhoII 168 GGATCT [GA]^GATC[CT] 725 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TseI Tsp45I TspRI