Restriction map for
pFN21AE1160
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BamHI BbeI
BbrPI BciVI BclI BfaI BfrI
BlnI BsaAI BseAI BsiWI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AhdI 1015 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1389 GTGCAC G^TGCAC
ApaI 763 GGGCCC GGGCC^C
ApaLI 1389 GTGCAC G^TGCAC
AspEI 1015 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1412 TGCGCA TGC^GCA
BbsI 498 GAAGAC GAAGAC(2/6)
BcgI 1362 CGAGATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 896 AGATCT A^GATCT
BlpI 267 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 267 GCTGAGC GC^T[ACGT]AGC
BpuAI 498 GAAGAC GAAGAC(2/6)
BsaBI 18 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1531 GAGACC GGTCTC(1/5)
BsiEI 1379 CGGCCG CG[GA][CT]^CG
BsmBI 1022 CGTCTC CGTCTC(1/5)
BsmI 1368 GAATGC GAATGC(1/-1)
BspMI 972 GCAGGT ACCTGC(4/8)
BsrDI 590 GCAATG GCAATG(2/0)
BstAPI 528 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 267 GCTGAGC GC^T[ACGT]AGC
EagI 1379 CGGCCG C^GGCCG
Eam1105I 1015 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1263 CTCTTC CTCTTC(1/4)
EclXI 1379 CGGCCG C^GGCCG
Eco52I 1379 CGGCCG C^GGCCG
EcoT14I 1430 CCAAGG C^C[AT][AT]GG
FspI 1412 TGCGCA TGC^GCA
Ksp632I 1263 CTCTTC CTCTTC(1/4)
MamI 18 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 724 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 369 GAGCTC GAGCT^C
SapI 1262 GCTCTTC GCTCTTC(1/4)
ScaI 1322 AGTACT AGT^ACT
SfiI 126 GGCCCACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 1430 CCAAGG C^C[AT][AT]GG
Van91I 724 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 1020 GGCGTC G[GA]^CG[CT]C
1459 GACGCC
AvaI 375 CCCGAG C^[CT]CG[GA]G
982 CCCGAG
BbvCI 243 CCTCAGC CCTCAGC(-5/-2)
1450 GCTGAGG
BpmI 49 CTGGAG CTGGAG(16/14)
1105 CTGGAG
BsaHI 1020 GGCGTC G[GA]^CG[CT]C
1459 GACGCC
BsaWI 675 TCCGGT [AT]^CCGG[AT]
1223 ACCGGA
BsgI 217 GTGCAG GTGCAG(16/14)
526 GTGCAG
BsmAI 1531 GAGAC GTCTC(1/5)
1023 GTCTC
BsoBI 375 CCCGAG C^[CT]CG[GA]G
982 CCCGAG
BspLU11I 905 ACATGT A^CATGT
1385 ACATGT
BsrBI 1260 CCGCTC CCGCTC(-3/-3)
778 GAGCGG
BsrI 225 CCAGT ACTGG(1/-1)
288 CCAGT
BssSI 1543 CACGAG CACGAG(-5/-1)
484 CTCGTG
HaeII 190 AGCGCC [GA]GCGC^[CT]
802 AGCGCC
Hin1I 1020 GGCGTC G[GA]^CG[CT]C
1459 GACGCC
MaeIII 89 GTGAC ^GT[ACGT]AC
1114 GTGAC
NaeI 207 GCCGGC GCC^GGC
263 GCCGGC
NgoMIV 207 GCCGGC G^CCGGC
263 GCCGGC
PvuII 1147 CAGCTG CAG^CTG
1242 CAGCTG
SphI 399 GCATGC GCATG^C
533 GCATGC
TaqI 1496 TCGA T^CGA
1529 TCGA
Tsp45I 89 GTGAC ^GT[GC]AC
1114 GTGAC
TthHB8I 1496 TCGA T^CGA
1529 TCGA
XcmI 567 CCAGAAGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
620 CCACGCCGGACCTGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
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AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbvI BcnI BglI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I Bsp1407I BsrFI
BsrGI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI TseI TspRI
XhoII