Restriction map for
pFN21AE4209
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiEI BsiWI Bsp1407I BspDI
BspHI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FseI FspI HindIII HpaI
MamI MfeI MluI MluNI MscI
MunI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 320 GACGTC GACGT^C
Acc65I 485 GGTACC G^GTACC
AccII 1785 CGCG CG^CG
AfaI 486 GTAC GT^AC
Asp718I 485 GGTACC G^GTACC
AvaI 383 CTCGAG C^[CT]CG[GA]G
BbeI 950 GGCGCC GGCGC^C
BbrPI 1746 CACGTG CAC^GTG
BbsI 1807 GAAGAC GAAGAC(2/6)
BglI 1238 GCCAGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1807 GAAGAC GAAGAC(2/6)
BsaAI 1746 CACGTG [CT]AC^GT[GA]
BsmBI 1260 CGTCTC CGTCTC(1/5)
BsmI 151 GAATGC GAATGC(1/-1)
BsoBI 383 CTCGAG C^[CT]CG[GA]G
BspLU11I 1148 ACATGT A^CATGT
BsrBI 568 CCGCTC CCGCTC(-3/-3)
BstUI 1785 CGCG CG^CG
EarI 1523 CTCTTC CTCTTC(1/4)
HincII 1474 GTCAAC GT[CT]^[GA]AC
HindII 1474 GTCAAC GT[CT]^[GA]AC
KasI 950 GGCGCC G^GCGCC
KpnI 485 GGTACC GGTAC^C
Ksp632I 1523 CTCTTC CTCTTC(1/4)
KspI 1784 CCGCGG CCGC^GG
MseI 437 TTAA T^TAA
MvnI 1785 CGCG CG^CG
NarI 950 GGCGCC GG^CGCC
NdeI 955 CATATG CA^TATG
NspI 1148 ACATGT [GA]CATG^[CT]
PaeR7I 383 CTCGAG C^TCGAG
PflMI 1014 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1746 CACGTG CAC^GTG
PmlI 1746 CACGTG CAC^GTG
PpuMI 1223 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1514 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 486 GTAC GT^AC
SacI 240 GAGCTC GAGCT^C
SacII 1784 CCGCGG CCGC^GG
SfoI 950 GGCGCC GGC^GCC
Tru9I 437 TTAA T^TAA
Van91I 1014 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 383 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 107 GTCTAC GT^[AC][GT]AC
846 GTCTAC
AflIII 842 ACACGT A^C[GA][CT]GT
1148 ACATGT
AocI 429 CCTAAGG CC^T[ACGT]AGG
2156 CCTCAGG
AspHI 240 GAGCTC G[AT]GC[AT]^C
979 GTGCTC
BbvCI 1642 CCTCAGC CCTCAGC(-5/-2)
1688 CCTCAGC
BcgI 278 CGATGACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2074 CGAAGTGGCTGC
BclI 665 TGATCA T^GATCA
1007 TGATCA
BfaI 346 CTAG C^TAG
1331 CTAG
BsgI 798 CTGCAC GTGCAG(16/14)
1162 CTGCAC
BsiHKAI 240 GAGCTC G[AT]GC[AT]^C
979 GTGCTC
BspMI 197 ACCTGC ACCTGC(4/8)
1199 ACCTGC
BsrFI 189 GCCGGC [GA]^CCGG[CT]
1495 GCCGGC
BssSI 205 CTCGTG CACGAG(-5/-1)
1597 CTCGTG
BstEII 424 GGTGACC G^GT[ACGT]ACC
1269 GGTCACC
BstPI 424 GGTGACC G^GT[ACGT]ACC
1269 GGTCACC
Bsu36I 429 CCTAAGG CC^T[ACGT]AGG
2156 CCTCAGG
Cfr10I 189 GCCGGC [GA]^CCGG[CT]
1495 GCCGGC
EaeI 191 CGGCCA [CT]^GGCC[GA]
1787 CGGCCA
Eco81I 429 CCTAAGG CC^T[ACGT]AGG
2156 CCTCAGG
EcoNI 260 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1200 CCTGCAAAAGG
EcoO65I 424 GGTGACC G^GT[ACGT]ACC
1269 GGTCACC
EcoRV 448 GATATC GAT^ATC
652 GATATC
FbaI 665 TGATCA T^GATCA
1007 TGATCA
HaeII 183 GGCGCT [GA]GCGC^[CT]
950 GGCGCC
MaeI 346 CTAG C^TAG
1331 CTAG
NaeI 189 GCCGGC GCC^GGC
1495 GCCGGC
NgoMIV 189 GCCGGC G^CCGGC
1495 GCCGGC
PleI 1342 GACTC GAGTC(4/5)
1280 GAGTC
PvuII 550 CAGCTG CAG^CTG
1044 CAGCTG
StuI 268 AGGCCT AGG^CCT
392 AGGCCT
TfiI 362 GAATC G^A[AT]TC
417 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccIII AciI AcyI AluI AlwI
ApaI AvaII BamHI BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspEI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MroI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII