Restriction map for
pFN21AE3525
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MslI MspA1I MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
TspRI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 314 GCGG CCGC(-3/-1)
AflII 45 CTTAAG C^TTAAG
BamHI 126 GGATCC G^GATCC
BanII 281 GGGCTC G[GA]GC[CT]^C
BbvCI 181 GCTGAGG CCTCAGC(-5/-2)
BcnI 579 CCCGG CC^[GC]GG
BfaI 530 CTAG C^TAG
BfrI 45 CTTAAG C^TTAAG
BmyI 281 GGGCTC G[AGT]GC[ACT]^C
BpmI 553 CTGGAG CTGGAG(16/14)
Bsp1286I 281 GGGCTC G[AGT]GC[ACT]^C
BstF5I 173 CATCC GGATG(2/0)
BstYI 126 GGATCC [GA]^GATC[CT]
Cac8I 133 GCAAGC GC[ACGT]^[ACGT]GC
EarI 595 GAAGAG CTCTTC(1/4)
FokI 173 CATCC GGATG(9/13)
HapII 580 CCGG C^CGG
HinfI 265 GAATC G^A[ACGT]TC
HpaII 580 CCGG C^CGG
HphI 342 TCACC GGTGA(8/7)
Ksp632I 595 GAAGAG CTCTTC(1/4)
MaeI 530 CTAG C^TAG
MaeII 246 ACGT A^CGT
MfeI 218 CAATTG C^AATTG
MflI 126 GGATCC [GA]^GATC[CT]
MspI 580 CCGG C^CGG
MunI 218 CAATTG C^AATTG
NciI 579 CCCGG CC^[GC]GG
NlaIV 126 GGATCC GG[ACGT]^[ACGT]CC
SfaNI 190 GATGC GCATC(5/9)
SfcI 251 CTGTAG C^T[GA][CT]AG
SmlI 45 CTTAAG C^T[CT][GA]AG
TfiI 265 GAATC G^A[AT]TC
XbaI 529 TCTAGA T^CTAGA
XcmI 486 CCATACCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 126 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbvI 193 GCTGC GCAGC(8/12)
202 GCTGC
DraII 548 AGGACCT [GA]G^G[ACGT]CC[CT]
617 AGGACCT
DsaI 176 CCATGG C^C[GA][CT]GG
197 CCATGG
EcoO109I 548 AGGACCT [GA]G^G[ACGT]CC[CT]
617 AGGACCT
EcoT14I 176 CCATGG C^C[AT][AT]GG
197 CCATGG
Fnu4HI 193 GCTGC GC^[ACGT]GC
202 GCTGC
HindIII 48 AAGCTT A^AGCTT
100 AAGCTT
ItaI 193 GCTGC GC^[ACGT]GC
202 GCTGC
MseI 46 TTAA T^TAA
320 TTAA
MwoI 193 GCTGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
196 GCCATGGCTGC
NcoI 176 CCATGG C^CATGG
197 CCATGG
PpuMI 548 AGGACCT [GA]G^G[AT]CC[CT]
617 AGGACCT
StyI 176 CCATGG C^C[AT][AT]GG
197 CCATGG
Tru9I 46 TTAA T^TAA
320 TTAA
TseI 193 GCTGC G^C[AT]GC
202 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwI AvaII BsaJI BseRI
BsiYI BslI BssKI BstNI Cfr13I
DdeI DpnII EcoRII MaeIII MboI
MboII MnlI MvaI NdeII NlaIII
Sau3AI Sau96I ScrFI Tsp509I