Restriction map for
pFN21AB4038
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 628 GTCTAC GT^[AC][GT]AC
AcsI 204 AAATTT [GA]^AATT[CT]
AlwNI 877 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 43 CCTGAGG CC^T[ACGT]AGG
ApaI 241 GGGCCC GGGCC^C
ApoI 204 AAATTT [GA]^AATT[CT]
AspI 669 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 892 GGTGCC G^G[CT][GA]CC
BbvCI 658 GCTGAGG CCTCAGC(-5/-2)
BsaI 667 GAGACC GGTCTC(1/5)
BsaWI 1082 TCCGGT [AT]^CCGG[AT]
BsgI 542 CTGCAC GTGCAG(16/14)
BspHI 302 TCATGA T^CATGA
BsrFI 1049 GCCGGC [GA]^CCGG[CT]
BssSI 437 CTCGTG CACGAG(-5/-1)
BstEII 1002 GGTAACC G^GT[ACGT]ACC
BstPI 1002 GGTAACC G^GT[ACGT]ACC
Bsu36I 43 CCTGAGG CC^T[ACGT]AGG
CfoI 1026 GCGC GCG^C
Cfr10I 1049 GCCGGC [GA]^CCGG[CT]
DrdI 691 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 43 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1002 GGTAACC G^GT[ACGT]ACC
EcoT14I 399 CCTTGG C^C[AT][AT]GG
FauI 245 CCCGC CCCGC(4/6)
HhaI 1026 GCGC GCG^C
HinP1I 1026 GCGC G^CGC
HincII 799 GTCAAC GT[CT]^[GA]AC
HindII 799 GTCAAC GT[CT]^[GA]AC
MslI 303 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 769 CAGCTG C[AC]G^C[GT]G
NaeI 1049 GCCGGC GCC^GGC
NgoMIV 1049 GCCGGC G^CCGGC
NspI 1074 GCATGT [GA]CATG^[CT]
PflFI 669 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 876 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 50 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 769 CAGCTG CAG^CTG
RcaI 302 TCATGA T^CATGA
SacI 298 GAGCTC GAGCT^C
SapI 379 GAAGAGC GCTCTTC(1/4)
SexAI 881 ACCTGGT A^CC[AT]GGT
SfaNI 75 GATGC GCATC(5/9)
SfcI 278 CTACAG C^T[GA][CT]AG
SmlI 324 CTTGAG C^T[CT][GA]AG
StyI 399 CCTTGG C^C[AT][AT]GG
TfiI 582 GAATC G^A[AT]TC
Tsp45I 844 GTGAC ^GT[GC]AC
Tsp509I 205 AATT ^AATT
Tth111I 669 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 876 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 228 CCAAGTAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 246 CCGC CCGC(-3/-1)
765 GCGG
AlwI 612 GGATC GGATC(4/5)
861 GGATC
AspHI 298 GAGCTC G[AT]GC[AT]^C
388 GTGCTC
BbsI 898 GAAGAC GAAGAC(2/6)
1078 GTCTTC
BcnI 369 CCGGG CC^[GC]GG
848 CCCGG
BglII 779 AGATCT A^GATCT
950 AGATCT
BpuAI 898 GAAGAC GAAGAC(2/6)
1078 GTCTTC
BsiHKAI 298 GAGCTC G[AT]GC[AT]^C
388 GTGCTC
BsmAI 667 GAGAC GTCTC(1/5)
606 GTCTC
BsrI 20 ACTGG ACTGG(1/-1)
672 CCAGT
BstYI 779 AGATCT [GA]^GATC[CT]
950 AGATCT
EarI 534 CTCTTC CTCTTC(1/4)
379 GAAGAG
Ksp632I 534 CTCTTC CTCTTC(1/4)
379 GAAGAG
MaeIII 844 GTGAC ^GT[ACGT]AC
1003 GTAAC
MflI 779 AGATCT [GA]^GATC[CT]
950 AGATCT
NciI 369 CCGGG CC^[GC]GG
848 CCCGG
TaqI 223 TCGA T^CGA
273 TCGA
TspRI 761 CAGTG CA[GC]TG[ACGT][ACGT]^
888 CAGTG
TthHB8I 223 TCGA T^CGA
273 TCGA
XhoII 779 AGATCT [GA]^GATC[CT]
950 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI AvaII BalI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BspMI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
StuI TseI