Restriction map for
pFN21AE4319
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BlnI BlpI Bpu1102I BsaHI BsaI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 58 GGTACC G^GTACC
AccIII 284 TCCGGA T^CCGGA
AlwI 664 GATCC GGATC(4/5)
AlwNI 469 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 58 GGTACC G^GTACC
BanII 639 GGGCTC G[GA]GC[CT]^C
BbrPI 605 CACGTG CAC^GTG
BbsI 288 GAAGAC GAAGAC(2/6)
BglI 632 GCCCTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 740 AGATCT A^GATCT
BmyI 639 GGGCTC G[AGT]GC[ACT]^C
BpuAI 288 GAAGAC GAAGAC(2/6)
BsaAI 605 CACGTG [CT]AC^GT[GA]
BsaBI 774 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 284 TCCGGA [AT]^CCGG[AT]
BseAI 284 TCCGGA T^CCGGA
Bsp1286I 639 GGGCTC G[AGT]GC[ACT]^C
BspEI 284 TCCGGA T^CCGGA
BsrBI 355 GAGCGG CCGCTC(-3/-3)
BsrDI 152 GCAATG GCAATG(2/0)
BstXI 600 CCAAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 740 AGATCT [GA]^GATC[CT]
CfoI 472 GCGC GCG^C
DraII 173 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 723 TGGCCG [CT]^GGCC[GA]
EcoO109I 173 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 931 CCAAGG C^C[AT][AT]GG
FauI 357 GCGGG CCCGC(4/6)
HaeII 471 GGCGCT [GA]GCGC^[CT]
HapII 285 CCGG C^CGG
HgaI 534 GCGTC GACGC(5/10)
HhaI 472 GCGC GCG^C
HinP1I 472 GCGC G^CGC
HpaII 285 CCGG C^CGG
HphI 836 TCACC GGTGA(8/7)
KpnI 58 GGTACC GGTAC^C
MamI 774 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 740 AGATCT [GA]^GATC[CT]
MroI 284 TCCGGA T^CCGGA
MslI 819 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 285 CCGG C^CGG
NlaIII 656 CATG CATG^
PflMI 236 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 605 CACGTG CAC^GTG
PmlI 605 CACGTG CAC^GTG
StyI 931 CCAAGG C^C[AT][AT]GG
TaqI 804 TCGA T^CGA
TfiI 806 GAATC G^A[AT]TC
Tsp45I 229 GTGAC ^GT[GC]AC
TthHB8I 804 TCGA T^CGA
Van91I 236 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 740 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 212 CTCGGG C^[CT]CG[GA]G
262 CCCGAG
BanI 58 GGTACC G^G[CT][GA]CC
207 GGTGCC
BseRI 642 CTCCTC GAGGAG(10/8)
217 GAGGAG
BsgI 22 CTGCAC GTGCAG(16/14)
466 GTGCAG
BsmAI 362 GAGAC GTCTC(1/5)
424 GAGAC
BsmI 381 GAATGC GAATGC(1/-1)
556 GAATGC
BsoBI 212 CTCGGG C^[CT]CG[GA]G
262 CCCGAG
BspMI 549 GCAGGT ACCTGC(4/8)
756 GCAGGT
Cac8I 276 GCCTGC GC[ACGT]^[ACGT]GC
468 GCAGGC
EcoNI 177 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
681 CCTGCTGGAGG
MaeII 188 ACGT A^CGT
606 ACGT
MaeIII 229 GTGAC ^GT[ACGT]AC
619 GTAAC
MseI 98 TTAA T^TAA
962 TTAA
Tru9I 98 TTAA T^TAA
962 TTAA
Tsp509I 588 AATT ^AATT
883 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MboI MboII MnlI MspA1I MvaI
MwoI NdeII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI TspRI