Restriction map for
pFN21AE3436
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp700I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII KasI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1155 GGTACC G^GTACC
AflIII 906 ACATGT A^C[GA][CT]GT
Alw44I 2116 GTGCAC G^TGCAC
AlwNI 2080 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 889 CCTGAGG CC^T[ACGT]AGG
ApaLI 2116 GTGCAC G^TGCAC
AseI 1221 ATTAAT AT^TAAT
AsnI 1221 ATTAAT AT^TAAT
Asp718I 1155 GGTACC G^GTACC
BanI 1155 GGTACC G^G[CT][GA]CC
BanII 1396 GAGCTC G[GA]GC[CT]^C
BbsI 677 GTCTTC GAAGAC(2/6)
BbvCI 1189 GCTGAGG CCTCAGC(-5/-2)
BglII 2775 AGATCT A^GATCT
BpuAI 677 GTCTTC GAAGAC(2/6)
BsaBI 949 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1195 GAATGC GAATGC(1/-1)
Bsp1407I 352 TGTACA T^GTACA
BspLU11I 906 ACATGT A^CATGT
BspMI 1617 ACCTGC ACCTGC(4/8)
BsrGI 352 TGTACA T^GTACA
BstXI 2581 CCAAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 889 CCTGAGG CC^T[ACGT]AGG
DraI 2769 TTTAAA TTT^AAA
DraII 653 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1697 TGGCCG [CT]^GGCC[GA]
EarI 24 GAAGAG CTCTTC(1/4)
Eco81I 889 CCTGAGG CC^T[ACGT]AGG
EcoNI 2608 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 653 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1579 GATATC GAT^ATC
HapII 1700 CCGG C^CGG
HpaI 276 GTTAAC GTT^AAC
HpaII 1700 CCGG C^CGG
HphI 1682 TCACC GGTGA(8/7)
KpnI 1155 GGTACC GGTAC^C
Ksp632I 24 GAAGAG CTCTTC(1/4)
MaeII 2660 ACGT A^CGT
MamI 949 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1700 CCGG C^CGG
PpuMI 653 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1221 ATTAAT AT^TAAT
SacI 1396 GAGCTC GAGCT^C
SalI 104 GTCGAC G^TCGAC
SfcI 2382 CTACAG C^T[GA][CT]AG
SmlI 2612 CTCAAG C^T[CT][GA]AG
SpeI 58 ACTAGT A^CTAGT
SspBI 352 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 104 GTCGAC GT^[AC][GT]AC
1487 GTCTAC
AciI 1507 GCGG CCGC(-3/-1)
1621 GCGG
AvaII 654 GGACC G^G[AT]CC
1623 GGTCC
BpmI 422 CTCCAG CTGGAG(16/14)
1828 CTGGAG
BsgI 992 GTGCAG GTGCAG(16/14)
1105 GTGCAG
BsmFI 653 GGGAC GGGAC(10/14)
2647 GGGAC
DsaI 1352 CCATGG C^C[GA][CT]GG
2252 CCATGG
MspA1I 47 CAGCTG C[AC]G^C[GT]G
1693 CAGCTG
NcoI 1352 CCATGG C^CATGG
2252 CCATGG
NheI 132 GCTAGC G^CTAGC
2758 GCTAGC
NspI 906 ACATGT [GA]CATG^[CT]
2354 ACATGC
PvuII 47 CAGCTG CAG^CTG
1693 CAGCTG
ScaI 28 AGTACT AGT^ACT
2230 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BbvI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII