Restriction map for
pFN21AB7845
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI AspEI
AviII AvrII BalI BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI Tsp509I
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 529 GGTACC G^GTACC
AccII 286 CGCG CG^CG
AflIII 1208 ACGTGT A^C[GA][CT]GT
AocI 718 CCTGAGG CC^T[ACGT]AGG
ApaI 906 GGGCCC GGGCC^C
Asp718I 529 GGTACC G^GTACC
AspI 1006 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 217 CCCGAG C^[CT]CG[GA]G
BamHI 302 GGATCC G^GATCC
BfaI 107 CTAG C^TAG
BsaAI 1207 TACGTG [CT]AC^GT[GA]
BsaWI 1346 ACCGGA [AT]^CCGG[AT]
BsmFI 1301 GGGAC GGGAC(10/14)
BsmI 1407 GAATGC GAATGC(1/-1)
BsoBI 217 CCCGAG C^[CT]CG[GA]G
BsrBI 1495 GAGCGG CCGCTC(-3/-3)
BstUI 286 CGCG CG^CG
BstXI 903 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 718 CCTGAGG CC^T[ACGT]AGG
DraI 694 TTTAAA TTT^AAA
DrdI 787 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 338 TGGCCG [CT]^GGCC[GA]
Eco81I 718 CCTGAGG CC^T[ACGT]AGG
EcoNI 499 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1353 GACGC GACGC(5/10)
HindIII 1563 AAGCTT A^AGCTT
HphI 60 GGTGA GGTGA(8/7)
KpnI 529 GGTACC GGTAC^C
MaeI 107 CTAG C^TAG
MaeII 1208 ACGT A^CGT
MaeIII 739 GTGAC ^GT[ACGT]AC
MvnI 286 CGCG CG^CG
NdeI 1454 CATATG CA^TATG
NspI 735 GCATGT [GA]CATG^[CT]
PflFI 1006 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 103 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1311 GAAGAGC GCTCTTC(1/4)
SgrAI 1426 CACCGGCG C[GA]^CCGG[CT]G
Tsp45I 739 GTGAC ^GT[GC]AC
Tth111I 1006 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 150 CCAGGAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 530 GTAC GT^AC
1164 GTAC
AlwNI 238 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1330 CAGCACCTG
BbsI 844 GAAGAC GAAGAC(2/6)
1599 GAAGAC
BcnI 306 CCGGG CC^[GC]GG
1361 CCGGG
BglI 947 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1411 GCCAATCTGGC
BpuAI 844 GAAGAC GAAGAC(2/6)
1599 GAAGAC
BsaI 638 GAGACC GGTCTC(1/5)
795 GGTCTC
BsgI 1212 GTGCAG GTGCAG(16/14)
1627 GTGCAG
BsrFI 1427 ACCGGC [GA]^CCGG[CT]
1589 ACCGGC
BstYI 239 AGATCC [GA]^GATC[CT]
302 GGATCC
Cfr10I 1427 ACCGGC [GA]^CCGG[CT]
1589 ACCGGC
DsaI 82 CCATGG C^C[GA][CT]GG
344 CCATGG
EarI 1111 GAAGAG CTCTTC(1/4)
1311 GAAGAG
HaeII 436 GGCGCT [GA]GCGC^[CT]
858 GGCGCT
Ksp632I 1111 GAAGAG CTCTTC(1/4)
1311 GAAGAG
MflI 239 AGATCC [GA]^GATC[CT]
302 GGATCC
MseI 5 TTAA T^TAA
695 TTAA
MslI 1426 CACCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1454 CATATGGGTG
NciI 306 CCGGG CC^[GC]GG
1361 CCGGG
NcoI 82 CCATGG C^CATGG
344 CCATGG
RsaI 530 GTAC GT^AC
1164 GTAC
SacI 184 GAGCTC GAGCT^C
1077 GAGCTC
SbfI 949 CCTGCAGG CCTGCA^GG
1335 CCTGCAGG
Sse8387I 949 CCTGCAGG CCTGCA^GG
1335 CCTGCAGG
Tru9I 5 TTAA T^TAA
695 TTAA
XhoII 239 AGATCC [GA]^GATC[CT]
302 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI Alw44I AlwI ApaLI
Asp700I AspHI AvaII BanI BanII
BbvCI BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI TspRI Van91I
XmnI