Restriction map for
pFN21AE4565
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BcgI BfrI
BlnI BlpI Bpu1102I BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BstAPI BstBI Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KasI KpnI MfeI MluI MroI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
Sse8387I SwaI Tsp45I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 21 CGCG CG^CG
AlwNI 1981 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 272 GGGCCC GGGCC^C
Asp700I 614 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1260 GTGCTC G[AT]GC[AT]^C
BalI 83 TGGCCA TGG^CCA
BanII 272 GGGCCC G[GA]GC[CT]^C
BbvCI 1231 GCTGAGG CCTCAGC(-5/-2)
BclI 1440 TGATCA T^GATCA
BglI 58 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 455 AGATCT A^GATCT
BsaAI 1807 TACGTG [CT]AC^GT[GA]
BsaBI 711 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1260 GTGCTC G[AT]GC[AT]^C
BsmAI 1983 GAGAC GTCTC(1/5)
Bsp1407I 2054 TGTACA T^GTACA
BspHI 954 TCATGA T^CATGA
BsrGI 2054 TGTACA T^GTACA
BssSI 403 CTCGTG CACGAG(-5/-1)
BstEII 748 GGTTACC G^GT[ACGT]ACC
BstPI 748 GGTTACC G^GT[ACGT]ACC
BstUI 21 CGCG CG^CG
DraI 1348 TTTAAA TTT^AAA
DraII 272 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1203 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 83 TGGCCA [CT]^GGCC[GA]
EarI 799 GAAGAG CTCTTC(1/4)
EcoO109I 272 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 748 GGTTACC G^GT[ACGT]ACC
FauI 19 CCCGC CCCGC(4/6)
FbaI 1440 TGATCA T^GATCA
HapII 277 CCGG C^CGG
HindIII 762 AAGCTT A^AGCTT
HpaII 277 CCGG C^CGG
Ksp632I 799 GAAGAG CTCTTC(1/4)
KspI 20 CCGCGG CCGC^GG
MamI 711 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 83 TGGCCA TGG^CCA
MscI 83 TGGCCA TGG^CCA
MspI 277 CCGG C^CGG
MvnI 21 CGCG CG^CG
NdeI 548 CATATG CA^TATG
PflMI 1098 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 990 CAGCTG CAG^CTG
RcaI 954 TCATGA T^CATGA
SacII 20 CCGCGG CCGC^GG
SapI 799 GAAGAGC GCTCTTC(1/4)
SmaI 276 CCCGGG CCC^GGG
SpeI 439 ACTAGT A^CTAGT
SphI 726 GCATGC GCATG^C
SspBI 2054 TGTACA T^GTACA
SspI 1136 AATATT AAT^ATT
Van91I 1098 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1275 CCAGGGAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 276 CCCGGG C^CCGGG
XmaI 276 CCCGGG C^CCGGG
XmnI 614 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 229 GGCGTC G[GA]^CG[CT]C
2033 GGCGTC
AvaI 31 CCCGAG C^[CT]CG[GA]G
276 CCCGGG
AvaII 940 GGACC G^G[AT]CC
1989 GGACC
BamHI 415 GGATCC G^GATCC
534 GGATCC
BbsI 1357 GAAGAC GAAGAC(2/6)
1859 GTCTTC
BciVI 524 GGATAC GTATCC(6/5)
691 GGATAC
BcnI 276 CCCGG CC^[GC]GG
277 CCGGG
BpuAI 1357 GAAGAC GAAGAC(2/6)
1859 GTCTTC
BsaHI 229 GGCGTC G[GA]^CG[CT]C
2033 GGCGTC
BsoBI 31 CCCGAG C^[CT]CG[GA]G
276 CCCGGG
BspMI 140 GCAGGT ACCTGC(4/8)
1855 GCAGGT
Bst1107I 1381 GTATAC GTA^TAC
1497 GTATAC
BstXI 86 CCAGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1318 CCAGATAAGTGG
BstZ17I 1381 GTATAC GTA^TAC
1497 GTATAC
HaeII 48 GGCGCT [GA]GCGC^[CT]
1400 GGCGCT
HgaI 230 GCGTC GACGC(5/10)
2034 GCGTC
Hin1I 229 GGCGTC G[GA]^CG[CT]C
2033 GGCGTC
NciI 276 CCCGG CC^[GC]GG
277 CCGGG
NspI 658 ACATGC [GA]CATG^[CT]
726 GCATGC
StuI 684 AGGCCT AGG^CCT
1802 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII