Restriction map for
pFN21AE4462
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvrII BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaBI BsaI BsiEI BsiHKAI Bsp1407I
BspDI BspHI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 12 CGCG CG^CG
AccIII 489 TCCGGA T^CCGGA
AflIII 1076 ACATGT A^C[GA][CT]GT
Asp700I 1320 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 971 TGCGCA TGC^GCA
BalI 119 TGGCCA TGG^CCA
BanII 274 GGGCTC G[GA]GC[CT]^C
BbeI 1357 GGCGCC GGCGC^C
BbsI 696 GTCTTC GAAGAC(2/6)
BlpI 1149 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1149 GCTTAGC GC^T[ACGT]AGC
BpuAI 696 GTCTTC GAAGAC(2/6)
BsaAI 1030 TACGTG [CT]AC^GT[GA]
BsaWI 489 TCCGGA [AT]^CCGG[AT]
BseAI 489 TCCGGA T^CCGGA
BsiWI 1028 CGTACG C^GTACG
BsmAI 1112 GTCTC GTCTC(1/5)
BsmBI 1111 CGTCTC CGTCTC(1/5)
BsmI 760 GAATGC GAATGC(1/-1)
BspEI 489 TCCGGA T^CCGGA
BspLU11I 1076 ACATGT A^CATGT
BsrDI 611 GCAATG GCAATG(2/0)
BstUI 12 CGCG CG^CG
BstXI 306 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1149 GCTTAGC GC^T[ACGT]AGC
DrdI 433 GACACTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 669 CCATGG C^C[GA][CT]GG
EcoT14I 669 CCATGG C^C[AT][AT]GG
FspI 971 TGCGCA TGC^GCA
HgaI 64 GCGTC GACGC(5/10)
KasI 1357 GGCGCC G^GCGCC
MaeII 1031 ACGT A^CGT
MluNI 119 TGGCCA TGG^CCA
MroI 489 TCCGGA T^CCGGA
MscI 119 TGGCCA TGG^CCA
MslI 879 CACTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 12 CGCG CG^CG
NarI 1357 GGCGCC GG^CGCC
NcoI 669 CCATGG C^CATGG
NspI 1076 ACATGT [GA]CATG^[CT]
PpuMI 249 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 258 CTGCAG CTGCA^G
ScaI 143 AGTACT AGT^ACT
SfcI 258 CTGCAG C^T[GA][CT]AG
SfoI 1357 GGCGCC GGC^GCC
SmaI 112 CCCGGG CCC^GGG
SmlI 925 CTCAAG C^T[CT][GA]AG
StyI 669 CCATGG C^C[AT][AT]GG
XmaCI 112 CCCGGG C^CCGGG
XmaI 112 CCCGGG C^CCGGG
XmnI 1320 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 652 GTCTAC GT^[AC][GT]AC
1192 GTAGAC
AcyI 63 GGCGTC G[GA]^CG[CT]C
1357 GGCGCC
BamHI 210 GGATCC G^GATCC
576 GGATCC
BanI 849 GGTGCC G^G[CT][GA]CC
1357 GGCGCC
BfaI 52 CTAG C^TAG
268 CTAG
BmyI 274 GGGCTC G[AGT]GC[ACT]^C
850 GTGCCC
BsaHI 63 GGCGTC G[GA]^CG[CT]C
1357 GGCGCC
BseRI 277 CTCCTC GAGGAG(10/8)
621 CTCCTC
BsgI 987 CTGCAC GTGCAG(16/14)
1258 GTGCAG
Bsp1286I 274 GGGCTC G[AGT]GC[ACT]^C
850 GTGCCC
Hin1I 63 GGCGTC G[GA]^CG[CT]C
1357 GGCGCC
HphI 320 TCACC GGTGA(8/7)
1400 TCACC
MaeI 52 CTAG C^TAG
268 CTAG
PflMI 891 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1245 CCAGAAGATGG
TfiI 348 GAATC G^A[AT]TC
1234 GATTC
Tsp45I 319 GTCAC ^GT[GC]AC
766 GTGAC
Tsp509I 454 AATT ^AATT
1207 AATT
Van91I 891 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1245 CCAGAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BbvI BcnI BpmI
BsaJI BsiYI BslI BsmFI BsoBI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI XhoII