Restriction map for
pFN21AE4406
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 7 GCGG CCGC(-3/-1)
AseI 553 ATTAAT AT^TAAT
AsnI 553 ATTAAT AT^TAAT
AspHI 943 GAGCAC G[AT]GC[AT]^C
AvaII 30 GGTCC G^G[AT]CC
BbsI 751 GTCTTC GAAGAC(2/6)
BclI 414 TGATCA T^GATCA
BmyI 943 GAGCAC G[AGT]GC[ACT]^C
BpuAI 751 GTCTTC GAAGAC(2/6)
BsaBI 622 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 451 GAGGAG GAGGAG(10/8)
BsgI 1117 GTGCAG GTGCAG(16/14)
BsiHKAI 943 GAGCAC G[AT]GC[AT]^C
BsmFI 223 GTCCC GGGAC(10/14)
Bsp1286I 943 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 346 TGTACA T^GTACA
BsrGI 346 TGTACA T^GTACA
BstBI 754 TTCGAA TT^CGAA
BstYI 627 AGATCC [GA]^GATC[CT]
DraII 29 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 236 GAAGAG CTCTTC(1/4)
EcoNI 487 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 29 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 689 GAATTC G^AATTC
FauI 7 GCGGG CCCGC(4/6)
FbaI 414 TGATCA T^GATCA
HaeIII 721 GGCC GG^CC
Ksp632I 236 GAAGAG CTCTTC(1/4)
MamI 622 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 627 AGATCC [GA]^GATC[CT]
MspA1I 5 CAGCGG C[AC]G^C[GT]G
NlaIII 717 CATG CATG^
NspV 754 TTCGAA TT^CGAA
PpuMI 29 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 553 ATTAAT AT^TAAT
SapI 236 GAAGAGC GCTCTTC(1/4)
SfuI 754 TTCGAA TT^CGAA
SspBI 346 TGTACA T^GTACA
Tsp45I 794 GTGAC ^GT[GC]AC
TspRI 185 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 899 CCAAGTATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 627 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 622 GATCC GGATC(4/5)
628 GATCC
Asp700I 38 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
918 GAAAACTTTC
BpmI 488 CTGGAG CTGGAG(16/14)
1082 CTGGAG
Cfr13I 30 GGTCC G^G[ACGT]CC
720 GGGCC
DdeI 34 CTGAG C^T[ACGT]AG
449 CTGAG
HinfI 662 GATTC G^A[ACGT]TC
1033 GATTC
MaeII 90 ACGT A^CGT
615 ACGT
MslI 180 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
458 CATTGATGTG
NlaIV 29 GGGTCC GG[ACGT]^[ACGT]CC
453 GGAGCC
PstI 24 CTGCAG CTGCA^G
191 CTGCAG
Sau96I 30 GGTCC G^G[ACGT]CC
720 GGGCC
TfiI 662 GATTC G^A[AT]TC
1033 GATTC
XmnI 38 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
918 GAAAACTTTC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
BbvI BfaI BsaJI BsiYI BslI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I DpnII EcoRII Fnu4HI FokI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII RsaI Sau3AI ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp509I
TthHB8I