Restriction map for
pFN21AE4240
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# NO CUT ENZYMES ( 88 enzymes) #
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AccIII AclI AcsI AflII AgeI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I AspI AviII
AvrII BamHI BbeI BbsI BcgI
BciVI BfrI BlnI BpuAI BsaBI
BsaI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII Bst1107I BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NheI NotI NruI NsiI PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfoI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1147 GACGTC GACGT^C
Acc65I 1844 GGTACC G^GTACC
AhdI 307 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1796 AGCGCT AGC^GCT
Asp718I 1844 GGTACC G^GTACC
AspEI 307 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1625 TGATCA T^GATCA
BfaI 1039 CTAG C^TAG
BlpI 1549 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1549 GCTGAGC GC^T[ACGT]AGC
BsaWI 338 TCCGGT [AT]^CCGG[AT]
BspLU11I 1967 ACATGT A^CATGT
BssSI 250 CTCGTG CACGAG(-5/-1)
BstBI 22 TTCGAA TT^CGAA
CelII 1549 GCTGAGC GC^T[ACGT]AGC
DraIII 2175 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 307 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1618 GAAGAG CTCTTC(1/4)
Eco47III 1796 AGCGCT AGC^GCT
EcoNI 2386 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1625 TGATCA T^GATCA
HaeII 1796 AGCGCT [GA]GCGC^[CT]
KpnI 1844 GGTACC GGTAC^C
Ksp632I 1618 GAAGAG CTCTTC(1/4)
MaeI 1039 CTAG C^TAG
MseI 2402 TTAA T^TAA
NaeI 2233 GCCGGC GCC^GGC
NdeI 1678 CATATG CA^TATG
NgoMIV 2233 GCCGGC G^CCGGC
NspV 22 TTCGAA TT^CGAA
PaeR7I 1279 CTCGAG C^TCGAG
PvuII 1288 CAGCTG CAG^CTG
SapI 1618 GAAGAGC GCTCTTC(1/4)
SbfI 1137 CCTGCAGG CCTGCA^GG
SexAI 1652 ACCTGGT A^CC[AT]GGT
SfiI 1096 GGCCAGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 22 TTCGAA TT^CGAA
SmaI 924 CCCGGG CCC^GGG
SmlI 1279 CTCGAG C^T[CT][GA]AG
Sse8387I 1137 CCTGCAGG CCTGCA^GG
Tru9I 2402 TTAA T^TAA
XhoI 1279 CTCGAG C^TCGAG
XmaCI 924 CCCGGG C^CCGGG
XmaI 924 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 1174 GTCTAC GT^[AC][GT]AC
2038 GTCTAC
AlwI 1413 GATCC GGATC(4/5)
1511 GGATC
BglI 177 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1097 GCCAGGGCGGC
BglII 1382 AGATCT A^GATCT
2192 AGATCT
BsiEI 1104 CGGCCG CG[GA][CT]^CG
1723 CGGCCG
BsmBI 1449 CGTCTC CGTCTC(1/5)
1995 CGTCTC
BstAPI 879 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1259 GCAAAGTCTGC
EagI 1104 CGGCCG C^GGCCG
1723 CGGCCG
EclXI 1104 CGGCCG C^GGCCG
1723 CGGCCG
Eco52I 1104 CGGCCG C^GGCCG
1723 CGGCCG
NcoI 436 CCATGG C^CATGG
1322 CCATGG
StuI 1367 AGGCCT AGG^CCT
2522 AGGCCT
TfiI 1071 GATTC G^A[AT]TC
1933 GAATC
Tsp509I 953 AATT ^AATT
996 AATT
XcmI 1211 CCAAGACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1298 CCAGCAACTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
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AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvCI BbvI BcnI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII