Restriction map for
pFN21AE4261
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccII AccIII AcyI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AvrII BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BlnI BsaAI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco52I EcoO109I
EcoO65I EcoRI FseI Hin1I KasI
KspI MamI MluI MroI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1055 GTCTAC GT^[AC][GT]AC
AclI 1284 AACGTT AA^CGTT
AflII 550 CTTAAG C^TTAAG
AflIII 169 ACATGT A^C[GA][CT]GT
AgeI 985 ACCGGT A^CCGGT
AocI 2175 CCTTAGG CC^T[ACGT]AGG
Aor51HI 291 AGCGCT AGC^GCT
AviII 2258 TGCGCA TGC^GCA
BalI 498 TGGCCA TGG^CCA
BamHI 2298 GGATCC G^GATCC
BfrI 550 CTTAAG C^TTAAG
BsaI 325 GGTCTC GGTCTC(1/5)
BsaWI 985 ACCGGT [AT]^CCGG[AT]
BsgI 2648 GTGCAG GTGCAG(16/14)
BspLU11I 169 ACATGT A^CATGT
BsrFI 985 ACCGGT [GA]^CCGG[CT]
Bsu36I 2175 CCTTAGG CC^T[ACGT]AGG
Cfr10I 985 ACCGGT [GA]^CCGG[CT]
DrdI 191 GACAGCTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1935 CCATGG C^C[GA][CT]GG
EaeI 498 TGGCCA [CT]^GGCC[GA]
EarI 3006 GAAGAG CTCTTC(1/4)
Eco47III 291 AGCGCT AGC^GCT
Eco81I 2175 CCTTAGG CC^T[ACGT]AGG
FauI 2752 GCGGG CCCGC(4/6)
FspI 2258 TGCGCA TGC^GCA
HaeII 291 AGCGCT [GA]GCGC^[CT]
HapII 986 CCGG C^CGG
HgaI 644 GACGC GACGC(5/10)
HindIII 1119 AAGCTT A^AGCTT
HpaI 2662 GTTAAC GTT^AAC
HpaII 986 CCGG C^CGG
Ksp632I 3006 GAAGAG CTCTTC(1/4)
MfeI 502 CAATTG C^AATTG
MluNI 498 TGGCCA TGG^CCA
MscI 498 TGGCCA TGG^CCA
MslI 170 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 986 CCGG C^CGG
MunI 502 CAATTG C^AATTG
NcoI 1935 CCATGG C^CATGG
PinAI 985 ACCGGT A^CCGGT
Psp1406I 1284 AACGTT AA^CGTT
SfcI 1344 CTATAG C^T[GA][CT]AG
SpeI 3018 ACTAGT A^CTAGT
SphI 2531 GCATGC GCATG^C
StuI 1913 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1034 GGTACC G^GTACC
1354 GGTACC
AlwNI 2539 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
2956 CAGTTTCTG
Asp718I 1034 GGTACC G^GTACC
1354 GGTACC
BanII 70 GAGCCC G[GA]GC[CT]^C
596 GAGCCC
BbsI 2368 GAAGAC GAAGAC(2/6)
472 GTCTTC
BglII 718 AGATCT A^GATCT
1193 AGATCT
BlpI 1230 GCTGAGC GC^T[ACGT]AGC
2408 GCTTAGC
BmyI 70 GAGCCC G[AGT]GC[ACT]^C
596 GAGCCC
BpmI 623 CTGGAG CTGGAG(16/14)
803 CTGGAG
Bpu1102I 1230 GCTGAGC GC^T[ACGT]AGC
2408 GCTTAGC
BpuAI 2368 GAAGAC GAAGAC(2/6)
472 GTCTTC
BsmAI 326 GTCTC GTCTC(1/5)
2080 GTCTC
Bsp1286I 70 GAGCCC G[AGT]GC[ACT]^C
596 GAGCCC
BspMI 228 GCAGGT ACCTGC(4/8)
1440 GCAGGT
BstXI 1143 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2385 CCATTTGATTGG
CelII 1230 GCTGAGC GC^T[ACGT]AGC
2408 GCTTAGC
CfoI 292 GCGC GCG^C
2259 GCGC
EcoNI 931 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1605 CCTAGAAAAGG
EcoT22I 17 ATGCAT ATGCA^T
369 ATGCAT
HhaI 292 GCGC GCG^C
2259 GCGC
HinP1I 292 GCGC G^CGC
2259 GCGC
HincII 1930 GTCAAC GT[CT]^[GA]AC
2662 GTTAAC
HindII 1930 GTCAAC GT[CT]^[GA]AC
2662 GTTAAC
KpnI 1034 GGTACC GGTAC^C
1354 GGTACC
MaeII 286 ACGT A^CGT
1285 ACGT
NsiI 17 ATGCAT ATGCA^T
369 ATGCAT
PleI 125 GACTC GAGTC(4/5)
3009 GAGTC
ScaI 1626 AGTACT AGT^ACT
1647 AGTACT
TaqI 329 TCGA T^CGA
2690 TCGA
TthHB8I 329 TCGA T^CGA
2690 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanI BbvI
BclI BfaI BsaJI BseRI BsiYI
BslI BsmFI BsmI Bsp1407I BspHI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoRV EcoT14I FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI