Restriction map for
pFN21AE4228
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsiWI
BsmI BspDI BspHI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MunI NdeI NheI NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I RcaI
RsrII SacI SacII ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 328 GTCGAC GT^[AC][GT]AC
AccIII 1025 TCCGGA T^CCGGA
AcsI 553 GAATTT [GA]^AATT[CT]
AocI 292 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1352 AGCGCT AGC^GCT
ApoI 553 GAATTT [GA]^AATT[CT]
Asp700I 1617 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaAI 1141 TACGTG [CT]AC^GT[GA]
BseAI 1025 TCCGGA T^CCGGA
Bsp1407I 20 TGTACA T^GTACA
BspEI 1025 TCCGGA T^CCGGA
BsrDI 449 GCAATG GCAATG(2/0)
BsrGI 20 TGTACA T^GTACA
BstEII 2133 GGTGACC G^GT[ACGT]ACC
BstPI 2133 GGTGACC G^GT[ACGT]ACC
Bsu36I 292 CCTAAGG CC^T[ACGT]AGG
DrdI 463 GACCCCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2360 CGGCCG C^GGCCG
EclXI 2360 CGGCCG C^GGCCG
Eco47III 1352 AGCGCT AGC^GCT
Eco52I 2360 CGGCCG C^GGCCG
Eco81I 292 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2133 GGTGACC G^GT[ACGT]ACC
HincII 328 GTCGAC GT[CT]^[GA]AC
HindII 328 GTCGAC GT[CT]^[GA]AC
MroI 1025 TCCGGA T^CCGGA
MslI 1372 CACGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 575 CCATGG C^CATGG
NruI 1808 TCGCGA TCG^CGA
NspI 848 GCATGC [GA]CATG^[CT]
PshAI 321 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 509 CGATCG CGAT^CG
SalI 328 GTCGAC G^TCGAC
SbfI 2139 CCTGCAGG CCTGCA^GG
SmaI 389 CCCGGG CCC^GGG
SphI 848 GCATGC GCATG^C
Sse8387I 2139 CCTGCAGG CCTGCA^GG
SspBI 20 TGTACA T^GTACA
StuI 596 AGGCCT AGG^CCT
Tsp509I 554 AATT ^AATT
XmaCI 389 CCCGGG C^CCGGG
XmaI 389 CCCGGG C^CCGGG
XmnI 1617 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 175 GTGCAC G^TGCAC
817 GTGCAC
ApaLI 175 GTGCAC G^TGCAC
817 GTGCAC
AspI 610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2121 GACCCGGTC
AvaI 389 CCCGGG C^[CT]CG[GA]G
2293 CCCGAG
BbeI 1363 GGCGCC GGCGC^C
1692 GGCGCC
BbvCI 231 CCTCAGC CCTCAGC(-5/-2)
1800 CCTCAGC
BsaI 502 GAGACC GGTCTC(1/5)
1009 GAGACC
BsaWI 1025 TCCGGA [AT]^CCGG[AT]
2026 ACCGGA
BsiEI 509 CGATCG CG[GA][CT]^CG
2360 CGGCCG
BsmBI 544 GAGACG CGTCTC(1/5)
781 GAGACG
BsoBI 389 CCCGGG C^[CT]CG[GA]G
2293 CCCGAG
BsrBI 1284 CCGCTC CCGCTC(-3/-3)
2244 GAGCGG
BstYI 356 AGATCC [GA]^GATC[CT]
429 GGATCT
KasI 1363 GGCGCC G^GCGCC
1692 GGCGCC
MflI 356 AGATCC [GA]^GATC[CT]
429 GGATCT
MseI 1172 TTAA T^TAA
1982 TTAA
NarI 1363 GGCGCC GG^CGCC
1692 GGCGCC
PflFI 610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2121 GACCCGGTC
PpuMI 608 GGGACCT [GA]G^G[AT]CC[CT]
2330 AGGACCT
SfoI 1363 GGCGCC GGC^GCC
1692 GGCGCC
SmlI 931 CTCAAG C^T[CT][GA]AG
2173 CTTGAG
Tru9I 1172 TTAA T^TAA
1982 TTAA
Tth111I 610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2121 GACCCGGTC
XcmI 959 CCAAGGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2384 CCAACTGCCTCCTGG
XhoII 356 AGATCC [GA]^GATC[CT]
429 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I