Restriction map for
pFN21AE4233
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AocI Aor51HI AscI AseI
AsnI Asp718I AspI AviII BamHI
BbrPI BclI BlpI BpmI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FbaI FseI FspI
HgaI HindIII HpaI KpnI KspI
MamI MluI MroI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 1378 GTAGAC GT^[AC][GT]AC
AccII 94 CGCG CG^CG
AflII 2311 CTTAAG C^TTAAG
AhdI 913 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1810 GTGCAC G^TGCAC
AlwNI 112 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 600 GGGCCC GGGCC^C
ApaLI 1810 GTGCAC G^TGCAC
AspEI 913 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 70 CCCGAG C^[CT]CG[GA]G
AvrII 804 CCTAGG C^CTAGG
BalI 561 TGGCCA TGG^CCA
BbvCI 1031 CCTCAGC CCTCAGC(-5/-2)
BcgI 1885 CGATGGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1161 GGATAC GTATCC(6/5)
BcnI 77 CCGGG CC^[GC]GG
BfrI 2311 CTTAAG C^TTAAG
BglI 116 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 804 CCTAGG C^CTAGG
BsaAI 955 TACGTG [CT]AC^GT[GA]
BsaI 920 GGTCTC GGTCTC(1/5)
BsgI 1105 CTGCAC GTGCAG(16/14)
BsoBI 70 CCCGAG C^[CT]CG[GA]G
Bsp1407I 522 TGTACA T^GTACA
BspDI 1312 ATCGAT AT^CGAT
BspHI 2472 TCATGA T^CATGA
BspMI 2227 GCAGGT ACCTGC(4/8)
BsrGI 522 TGTACA T^GTACA
BstAPI 111 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 684 GGTAACC G^GT[ACGT]ACC
BstPI 684 GGTAACC G^GT[ACGT]ACC
BstUI 94 CGCG CG^CG
ClaI 1312 ATCGAT AT^CGAT
DraII 600 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 540 CCATGG C^C[GA][CT]GG
EaeI 561 TGGCCA [CT]^GGCC[GA]
Eam1105I 913 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 600 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 684 GGTAACC G^GT[ACGT]ACC
EcoRV 1438 GATATC GAT^ATC
FauI 120 GCGGG CCCGC(4/6)
HapII 77 CCGG C^CGG
HincII 427 GTTGAC GT[CT]^[GA]AC
HindII 427 GTTGAC GT[CT]^[GA]AC
HpaII 77 CCGG C^CGG
MluNI 561 TGGCCA TGG^CCA
MscI 561 TGGCCA TGG^CCA
MspI 77 CCGG C^CGG
MvnI 94 CGCG CG^CG
NciI 77 CCGGG CC^[GC]GG
NcoI 540 CCATGG C^CATGG
NruI 93 TCGCGA TCG^CGA
NspI 535 GCATGC [GA]CATG^[CT]
PvuII 1034 CAGCTG CAG^CTG
RcaI 2472 TCATGA T^CATGA
ScaI 971 AGTACT AGT^ACT
SphI 535 GCATGC GCATG^C
SspBI 522 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 12 GGCGCC G[GA]^CG[CT]C
42 GGCGCC
Asp700I 839 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2282 GAATTTATTC
BanI 12 GGCGCC G^G[CT][GA]CC
42 GGCGCC
BbeI 12 GGCGCC GGCGC^C
42 GGCGCC
BglII 128 AGATCT A^GATCT
342 AGATCT
BsaHI 12 GGCGCC G[GA]^CG[CT]C
42 GGCGCC
BseRI 1293 CTCCTC GAGGAG(10/8)
733 GAGGAG
BstXI 194 CCATAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1133 CCACTGCTGTGG
EcoT14I 540 CCATGG C^C[AT][AT]GG
804 CCTAGG
HaeII 12 GGCGCC [GA]GCGC^[CT]
42 GGCGCC
Hin1I 12 GGCGCC G[GA]^CG[CT]C
42 GGCGCC
KasI 12 GGCGCC G^GCGCC
42 GGCGCC
MfeI 1397 CAATTG C^AATTG
2531 CAATTG
MspA1I 47 CAGCGG C[AC]G^C[GT]G
1034 CAGCTG
MunI 1397 CAATTG C^AATTG
2531 CAATTG
NarI 12 GGCGCC GG^CGCC
42 GGCGCC
SfoI 12 GGCGCC GGC^GCC
42 GGCGCC
SmlI 1844 CTCAAG C^T[CT][GA]AG
2311 CTTAAG
StyI 540 CCATGG C^C[AT][AT]GG
804 CCTAGG
Tsp45I 572 GTGAC ^GT[GC]AC
1289 GTGAC
XmnI 839 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2282 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XhoII