Restriction map for
pFN21AE3185
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BclI BcnI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoRI
EcoT22I FbaI FseI FspI HgaI
Hin1I HpaI KasI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 481 GGTACC G^GTACC
AccI 381 GTCTAC GT^[AC][GT]AC
Alw44I 1084 GTGCAC G^TGCAC
AlwNI 718 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 860 AGCGCT AGC^GCT
ApaLI 1084 GTGCAC G^TGCAC
Asp718I 481 GGTACC G^GTACC
AspHI 1084 GTGCAC G[AT]GC[AT]^C
AvaI 344 CCCGAG C^[CT]CG[GA]G
BbvCI 737 CCTCAGC CCTCAGC(-5/-2)
BcgI 504 CGAGCGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 899 GTATCC GTATCC(6/5)
BglI 181 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 128 AGATCT A^GATCT
BsaWI 1256 TCCGGT [AT]^CCGG[AT]
BseRI 1123 GAGGAG GAGGAG(10/8)
BsiEI 228 CGGCCG CG[GA][CT]^CG
BsiHKAI 1084 GTGCAC G[AT]GC[AT]^C
BsoBI 344 CCCGAG C^[CT]CG[GA]G
Bsp1407I 785 TGTACA T^GTACA
BsrGI 785 TGTACA T^GTACA
CfoI 861 GCGC GCG^C
DsaI 485 CCATGG C^C[GA][CT]GG
EaeI 228 CGGCCG [CT]^GGCC[GA]
EagI 228 CGGCCG C^GGCCG
EclXI 228 CGGCCG C^GGCCG
Eco47III 860 AGCGCT AGC^GCT
Eco52I 228 CGGCCG C^GGCCG
EcoRV 1252 GATATC GAT^ATC
FauI 879 GCGGG CCCGC(4/6)
HaeII 860 AGCGCT [GA]GCGC^[CT]
HhaI 861 GCGC GCG^C
HinP1I 861 GCGC G^CGC
HincII 111 GTTGAC GT[CT]^[GA]AC
HindII 111 GTTGAC GT[CT]^[GA]AC
HindIII 592 AAGCTT A^AGCTT
KpnI 481 GGTACC GGTAC^C
NcoI 485 CCATGG C^CATGG
NotI 227 GCGGCCGC GC^GGCCGC
PflMI 717 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1006 GACTC GAGTC(4/5)
PstI 512 CTGCAG CTGCA^G
SapI 331 GAAGAGC GCTCTTC(1/4)
SphI 729 GCATGC GCATG^C
SspBI 785 TGTACA T^GTACA
TfiI 920 GATTC G^A[AT]TC
Van91I 717 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1169 CCAGTGTGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 771 GGATC GGATC(4/5)
1266 GGATC
ApaI 360 GGGCCC GGGCC^C
491 GGGCCC
BbvI 733 GCAGC GCAGC(8/12)
831 GCTGC
BfaI 49 CTAG C^TAG
1054 CTAG
BpmI 1208 CTCCAG CTGGAG(16/14)
1180 CTGGAG
BsrBI 225 GAGCGG CCGCTC(-3/-3)
505 GAGCGG
BsrI 115 ACTGG ACTGG(1/-1)
1169 CCAGT
BstEII 712 GGTTACC G^GT[ACGT]ACC
1164 GGTGACC
BstPI 712 GGTTACC G^GT[ACGT]ACC
1164 GGTGACC
BstXI 365 CCACTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
434 CCACTTATGTGG
BstYI 128 AGATCT [GA]^GATC[CT]
1266 GGATCT
EcoO65I 712 GGTTACC G^GT[ACGT]ACC
1164 GGTGACC
HapII 876 CCGG C^CGG
1257 CCGG
HinfI 920 GATTC G^A[ACGT]TC
1006 GACTC
HpaII 876 CCGG C^CGG
1257 CCGG
MaeI 49 CTAG C^TAG
1054 CTAG
MflI 128 AGATCT [GA]^GATC[CT]
1266 GGATCT
MseI 683 TTAA T^TAA
950 TTAA
MspI 876 CCGG C^CGG
1257 CCGG
MwoI 181 GCCAAGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
733 GCAGCCTCAGC
NspI 729 GCATGC [GA]CATG^[CT]
1025 GCATGT
PpuMI 339 AGGTCCC [GA]G^G[AT]CC[CT]
803 GGGACCT
SfcI 383 CTACAG C^T[GA][CT]AG
512 CTGCAG
SmlI 1031 CTCAAG C^T[CT][GA]AG
1048 CTCAAG
TaqI 503 TCGA T^CGA
521 TCGA
Tru9I 683 TTAA T^TAA
950 TTAA
TseI 733 GCAGC G^C[AT]GC
831 GCTGC
Tsp45I 1063 GTCAC ^GT[GC]AC
1165 GTGAC
TthHB8I 503 TCGA T^CGA
521 TCGA
XhoII 128 AGATCT [GA]^GATC[CT]
1266 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanI BanII BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tsp509I TspRI