Restriction map for
pFN21AE4132
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbeI BbrPI
BciVI BclI BfaI BfrI BlnI
BsaBI BsaHI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MaeI MamI MfeI MluI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 1215 GGTACC G^GTACC
AccIII 97 TCCGGA T^CCGGA
AhdI 74 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 701 GGGCCC GGGCC^C
Asp718I 1215 GGTACC G^GTACC
AspEI 74 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 986 GAAGAC GAAGAC(2/6)
BbvCI 207 CCTCAGC CCTCAGC(-5/-2)
BcgI 1477 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 558 AGATCT A^GATCT
BpuAI 986 GAAGAC GAAGAC(2/6)
BsaAI 1114 TACGTG [CT]AC^GT[GA]
BsaWI 97 TCCGGA [AT]^CCGG[AT]
BseAI 97 TCCGGA T^CCGGA
BspEI 97 TCCGGA T^CCGGA
BstXI 857 CCAACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 558 AGATCT [GA]^GATC[CT]
DrdI 447 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 239 CCATGG C^C[GA][CT]GG
Eam1105I 74 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 165 CTCTTC CTCTTC(1/4)
HgaI 779 GACGC GACGC(5/10)
HindIII 1299 AAGCTT A^AGCTT
KpnI 1215 GGTACC GGTAC^C
Ksp632I 165 CTCTTC CTCTTC(1/4)
MflI 558 AGATCT [GA]^GATC[CT]
MroI 97 TCCGGA T^CCGGA
MslI 991 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 239 CCATGG C^CATGG
NspI 497 GCATGT [GA]CATG^[CT]
SapI 164 GCTCTTC GCTCTTC(1/4)
SmaI 704 CCCGGG CCC^GGG
SmlI 1180 CTTGAG C^T[CT][GA]AG
TaqI 933 TCGA T^CGA
TfiI 22 GAATC G^A[AT]TC
TthHB8I 933 TCGA T^CGA
XhoII 558 AGATCT [GA]^GATC[CT]
XmaCI 704 CCCGGG C^CCGGG
XmaI 704 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 64 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1052 CAGCACCTG
AvaII 66 GGACC G^G[AT]CC
1290 GGACC
BanI 1215 GGTACC G^G[CT][GA]CC
1344 GGCACC
BglI 349 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
406 GCCTTTGTGGC
BlpI 358 GCTGAGC GC^T[ACGT]AGC
1296 GCTAAGC
BpmI 270 CTGGAG CTGGAG(16/14)
1044 CTGGAG
Bpu1102I 358 GCTGAGC GC^T[ACGT]AGC
1296 GCTAAGC
BsaI 445 GAGACC GGTCTC(1/5)
1018 GAGACC
BsmBI 81 CGTCTC CGTCTC(1/5)
606 GAGACG
BsrBI 9 GAGCGG CCGCTC(-3/-3)
1119 GAGCGG
CelII 358 GCTGAGC GC^T[ACGT]AGC
1296 GCTAAGC
DpnII 54 GATC ^GATC
559 GATC
MaeII 1115 ACGT A^CGT
1195 ACGT
MboI 54 GATC ^GATC
559 GATC
MboII 986 GAAGA GAAGA(8/7)
166 TCTTC
NdeII 54 GATC ^GATC
559 GATC
PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT]
1289 AGGACCT
PstI 151 CTGCAG CTGCA^G
1387 CTGCAG
Sau3AI 54 GATC ^GATC
559 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
BalI BanII BbvI BcnI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PflMI PleI
PvuII RsaI SacI Sau96I ScrFI
SfaNI SfcI StuI StyI TseI
Tsp45I TspRI Van91I