Restriction map for
pFN21AE4117
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BseAI
BsgI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CpoI DraI DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI Fnu4HI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI ItaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 508 GAATTT [GA]^AATT[CT]
AflII 253 CTTAAG C^TTAAG
AflIII 1134 ACACGT A^C[GA][CT]GT
AlwNI 1340 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 508 GAATTT [GA]^AATT[CT]
BamHI 970 GGATCC G^GATCC
BanI 30 GGTGCC G^G[CT][GA]CC
BanII 1250 GGGCTC G[GA]GC[CT]^C
BfaI 550 CTAG C^TAG
BfrI 253 CTTAAG C^TTAAG
BlpI 229 GCTCAGC GC^T[ACGT]AGC
BmyI 1250 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 229 GCTCAGC GC^T[ACGT]AGC
BsaWI 293 TCCGGT [AT]^CCGG[AT]
BsiEI 48 CGGCCG CG[GA][CT]^CG
BsmFI 342 GTCCC GGGAC(10/14)
Bsp1286I 1250 GGGCTC G[AGT]GC[ACT]^C
BspDI 205 ATCGAT AT^CGAT
BspHI 1140 TCATGA T^CATGA
BsrFI 665 ACCGGC [GA]^CCGG[CT]
BsrI 684 CCAGT ACTGG(1/-1)
BstYI 970 GGATCC [GA]^GATC[CT]
CelII 229 GCTCAGC GC^T[ACGT]AGC
CfoI 1260 GCGC GCG^C
Cfr10I 665 ACCGGC [GA]^CCGG[CT]
ClaI 205 ATCGAT AT^CGAT
DraIII 14 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 48 CGGCCG C^GGCCG
EclXI 48 CGGCCG C^GGCCG
Eco52I 48 CGGCCG C^GGCCG
HhaI 1260 GCGC GCG^C
HinP1I 1260 GCGC G^CGC
MaeI 550 CTAG C^TAG
MflI 970 GGATCC [GA]^GATC[CT]
PleI 1153 GAGTC GAGTC(4/5)
PstI 66 CTGCAG CTGCA^G
RcaI 1140 TCATGA T^CATGA
SapI 690 GCTCTTC GCTCTTC(1/4)
SfcI 66 CTGCAG C^T[GA][CT]AG
XhoII 970 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 40 CGCG CG^CG
1259 CGCG
AfaI 430 GTAC GT^AC
1240 GTAC
AlwI 971 GATCC GGATC(4/5)
970 GGATC
BalI 829 TGGCCA TGG^CCA
1349 TGGCCA
BcnI 433 CCGGG CC^[GC]GG
1062 CCGGG
BsaI 1090 GAGACC GGTCTC(1/5)
296 GGTCTC
BseRI 707 CTCCTC GAGGAG(10/8)
1087 GAGGAG
BsrDI 363 CATTGC GCAATG(2/0)
83 GCAATG
BstUI 40 CGCG CG^CG
1259 CGCG
DraII 340 GGGTCCC [GA]G^G[ACGT]CC[CT]
1289 AGGTCCT
EarI 691 CTCTTC CTCTTC(1/4)
1108 GAAGAG
EcoO109I 340 GGGTCCC [GA]G^G[ACGT]CC[CT]
1289 AGGTCCT
FauI 38 CCCGC CCCGC(4/6)
266 GCGGG
HphI 1357 GGTGA GGTGA(8/7)
1080 TCACC
Ksp632I 691 CTCTTC CTCTTC(1/4)
1108 GAAGAG
MaeII 821 ACGT A^CGT
1136 ACGT
MaeIII 841 GTCAC ^GT[ACGT]AC
1417 GTCAC
MluNI 829 TGGCCA TGG^CCA
1349 TGGCCA
MscI 829 TGGCCA TGG^CCA
1349 TGGCCA
MvnI 40 CGCG CG^CG
1259 CGCG
NciI 433 CCGGG CC^[GC]GG
1062 CCGGG
PpuMI 340 GGGTCCC [GA]G^G[AT]CC[CT]
1289 AGGTCCT
RsaI 430 GTAC GT^AC
1240 GTAC
SmlI 253 CTTAAG C^T[CT][GA]AG
310 CTCAAG
TaqI 99 TCGA T^CGA
206 TCGA
Tsp45I 841 GTCAC ^GT[GC]AC
1417 GTCAC
TthHB8I 99 TCGA T^CGA
206 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AvaII BpmI BsaJI
BsiYI BslI BsmAI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII FokI HaeIII HapII
HinfI HpaII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I Tsp509I TspRI