Restriction map for
pFN21AE4108
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# NO CUT ENZYMES ( 104 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI AhdI AlwI ApoI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BbvCI BciVI BfrI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KspI MamI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI TfiI Tru9I Tsp509I Van91I
XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 982 GACGTC GACGT^C
Acc65I 56 GGTACC G^GTACC
AocI 938 CCTCAGG CC^T[ACGT]AGG
Aor51HI 209 AGCGCT AGC^GCT
Asp700I 369 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 56 GGTACC G^GTACC
AspI 346 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 985 GTCTTC GAAGAC(2/6)
BcgI 449 GCAAGAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 479 TGATCA T^GATCA
BglI 493 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 985 GTCTTC GAAGAC(2/6)
BsgI 1351 GTGCAG GTGCAG(16/14)
BsiEI 1104 CGGCCG CG[GA][CT]^CG
BsrBI 1237 GAGCGG CCGCTC(-3/-3)
BsrDI 579 GCAATG GCAATG(2/0)
BsrI 1194 CCAGT ACTGG(1/-1)
Bsu36I 938 CCTCAGG CC^T[ACGT]AGG
DsaI 488 CCGTGG C^C[GA][CT]GG
EagI 1104 CGGCCG C^GGCCG
EclXI 1104 CGGCCG C^GGCCG
Eco47III 209 AGCGCT AGC^GCT
Eco52I 1104 CGGCCG C^GGCCG
Eco81I 938 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1214 CCAAGG C^C[AT][AT]GG
FbaI 479 TGATCA T^GATCA
HinfI 1303 GACTC G^A[ACGT]TC
KpnI 56 GGTACC GGTAC^C
NotI 1103 GCGGCCGC GC^GGCCGC
PaeR7I 1399 CTCGAG C^TCGAG
PflFI 346 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1303 GACTC GAGTC(4/5)
SacI 754 GAGCTC GAGCT^C
SapI 1167 GCTCTTC GCTCTTC(1/4)
SbfI 281 CCTGCAGG CCTGCA^GG
SfiI 492 GGCCTTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 281 CCTGCAGG CCTGCA^GG
StyI 1214 CCAAGG C^C[AT][AT]GG
Tth111I 346 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 324 TCTAGA T^CTAGA
XhoI 1399 CTCGAG C^TCGAG
XmnI 369 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 645 GGCGTC G[GA]^CG[CT]C
982 GACGTC
Alw44I 401 GTGCAC G^TGCAC
1197 GTGCAC
ApaI 500 GGGCCC GGGCC^C
945 GGGCCC
ApaLI 401 GTGCAC G^TGCAC
1197 GTGCAC
BanI 56 GGTACC G^G[CT][GA]CC
470 GGCACC
BfaI 325 CTAG C^TAG
1126 CTAG
BlpI 600 GCTCAGC GC^T[ACGT]AGC
1056 GCTGAGC
BpmI 64 CTGGAG CTGGAG(16/14)
610 CTGGAG
Bpu1102I 600 GCTCAGC GC^T[ACGT]AGC
1056 GCTGAGC
BsaHI 645 GGCGTC G[GA]^CG[CT]C
982 GACGTC
BsmAI 74 GAGAC GTCTC(1/5)
1297 GAGAC
BsmBI 74 GAGACG CGTCTC(1/5)
1297 GAGACG
Bsp1407I 518 TGTACA T^GTACA
1010 TGTACA
BspLU11I 1007 ACATGT A^CATGT
1070 ACATGT
BsrGI 518 TGTACA T^GTACA
1010 TGTACA
BssSI 142 CACGAG CACGAG(-5/-1)
1411 CTCGTG
BstAPI 403 GCACGTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1383 GCAGCGTCTGC
BstXI 422 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1418 CCATCGGTCTGG
CelII 600 GCTCAGC GC^T[ACGT]AGC
1056 GCTGAGC
DpnII 480 GATC ^GATC
766 GATC
DraIII 227 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
404 CACGTAGTG
EarI 1168 CTCTTC CTCTTC(1/4)
1092 GAAGAG
EcoNI 281 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
708 CCTCCGGGAGG
Hin1I 645 GGCGTC G[GA]^CG[CT]C
982 GACGTC
Ksp632I 1168 CTCTTC CTCTTC(1/4)
1092 GAAGAG
MaeI 325 CTAG C^TAG
1126 CTAG
MboI 480 GATC ^GATC
766 GATC
NdeII 480 GATC ^GATC
766 GATC
PpuMI 593 GGGACCT [GA]G^G[AT]CC[CT]
1403 AGGACCT
Sau3AI 480 GATC ^GATC
766 GATC
SmlI 1186 CTTGAG C^T[CT][GA]AG
1399 CTCGAG
SspBI 518 TGTACA T^GTACA
1010 TGTACA
StuI 860 AGGCCT AGG^CCT
1086 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AvaI AvaII BalI
BanII BbrPI BbvI BcnI BmyI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV NspI
PmaCI PmlI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I