Restriction map for
pFN21AE4339
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflII AflIII AgeI AhdI
AocI Aor51HI AscI Asp700I Asp718I
AspEI AspI AviII BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BsaAI BsaBI BsaHI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI PvuII
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 343 TCCGGA T^CCGGA
Alw44I 803 GTGCAC G^TGCAC
AlwNI 460 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 728 GGGCCC GGGCC^C
ApaLI 803 GTGCAC G^TGCAC
AseI 409 ATTAAT AT^TAAT
AsnI 409 ATTAAT AT^TAAT
AvaI 104 CTCGGG C^[CT]CG[GA]G
AvaII 141 GGACC G^G[AT]CC
AvrII 114 CCTAGG C^CTAGG
BalI 719 TGGCCA TGG^CCA
BamHI 7 GGATCC G^GATCC
BglI 721 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 114 CCTAGG C^CTAGG
BlpI 211 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 211 GCTCAGC GC^T[ACGT]AGC
BsaWI 343 TCCGGA [AT]^CCGG[AT]
BseAI 343 TCCGGA T^CCGGA
BseRI 271 GAGGAG GAGGAG(10/8)
BsgI 325 CTGCAC GTGCAG(16/14)
BsiEI 206 CGGCCG CG[GA][CT]^CG
BsoBI 104 CTCGGG C^[CT]CG[GA]G
BspEI 343 TCCGGA T^CCGGA
BspMI 628 GCAGGT ACCTGC(4/8)
BsrBI 209 CCGCTC CCGCTC(-3/-3)
BstAPI 574 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 7 GGATCC [GA]^GATC[CT]
Cac8I 920 GCGTGC GC[ACGT]^[ACGT]GC
CelII 211 GCTCAGC GC^T[ACGT]AGC
EagI 206 CGGCCG C^GGCCG
EclXI 206 CGGCCG C^GGCCG
Eco52I 206 CGGCCG C^GGCCG
EcoRI 990 GAATTC G^AATTC
HgaI 293 GACGC GACGC(5/10)
MaeII 944 ACGT A^CGT
MaeIII 742 GTGAC ^GT[ACGT]AC
MflI 7 GGATCC [GA]^GATC[CT]
MluNI 719 TGGCCA TGG^CCA
MroI 343 TCCGGA T^CCGGA
MscI 719 TGGCCA TGG^CCA
MseI 410 TTAA T^TAA
MspA1I 147 CCGCTG C[AC]G^C[GT]G
NcoI 288 CCATGG C^CATGG
PflMI 459 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 639 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 409 ATTAAT AT^TAAT
SacI 955 GAGCTC GAGCT^C
SfaNI 655 GATGC GCATC(5/9)
SfiI 720 GGCCAATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 256 CTCAAG C^T[CT][GA]AG
TaqI 44 TCGA T^CGA
Tru9I 410 TTAA T^TAA
Tsp45I 742 GTGAC ^GT[GC]AC
TspRI 764 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 44 TCGA T^CGA
Van91I 459 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 7 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 83 CGCG CG^CG
159 CGCG
BbsI 708 GAAGAC GAAGAC(2/6)
844 GAAGAC
BcnI 4 CCGGG CC^[GC]GG
267 CCGGG
BfaI 115 CTAG C^TAG
421 CTAG
BpuAI 708 GAAGAC GAAGAC(2/6)
844 GAAGAC
BsrDI 450 CATTGC GCAATG(2/0)
925 CATTGC
BsrI 765 ACTGG ACTGG(1/-1)
387 CCAGT
BstUI 83 CGCG CG^CG
159 CGCG
CfoI 84 GCGC GCG^C
126 GCGC
DdeI 212 CTCAG C^T[ACGT]AG
733 CTGAG
DraII 727 GGGGCCC [GA]G^G[ACGT]CC[CT]
728 GGGCCCT
DsaI 230 CCGTGG C^C[GA][CT]GG
288 CCATGG
EaeI 206 CGGCCG [CT]^GGCC[GA]
719 TGGCCA
EcoNI 351 CCTAAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
531 CCTCAAAGAGG
EcoO109I 727 GGGGCCC [GA]G^G[ACGT]CC[CT]
728 GGGCCCT
EcoT14I 114 CCTAGG C^C[AT][AT]GG
288 CCATGG
FauI 69 GCGGG CCCGC(4/6)
160 GCGGG
HhaI 84 GCGC GCG^C
126 GCGC
HinP1I 84 GCGC G^CGC
126 GCGC
HincII 336 GTTGAC GT[CT]^[GA]AC
685 GTCAAC
HindII 336 GTTGAC GT[CT]^[GA]AC
685 GTCAAC
MaeI 115 CTAG C^TAG
421 CTAG
MslI 478 CACTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1033 CATCCTGATG
MvnI 83 CGCG CG^CG
159 CGCG
NciI 4 CCGGG CC^[GC]GG
267 CCGGG
StuI 348 AGGCCT AGG^CCT
524 AGGCCT
StyI 114 CCTAGG C^C[AT][AT]GG
288 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BanII BbvI BclI BmyI
BpmI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BssKI BstF5I BstNI Cfr13I DpnII
EcoRII FbaI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RcaI Sau3AI Sau96I ScrFI TseI
Tsp509I