Restriction map for
pFN21AE4248
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccIII AclI AflII
AhdI Aor51HI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BbvCI
BciVI BclI BfrI BlnI BsaBI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HpaI KpnI MamI MluI MroI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI Psp1406I PvuI
RsrII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AccI 2761 GTCTAC GT^[AC][GT]AC
AflIII 1787 ACGTGT A^C[GA][CT]GT
AgeI 69 ACCGGT A^CCGGT
Alw44I 982 GTGCAC G^TGCAC
ApaLI 982 GTGCAC G^TGCAC
BalI 1121 TGGCCA TGG^CCA
BfaI 2320 CTAG C^TAG
BglII 2606 AGATCT A^GATCT
BsmI 894 GCATTC GAATGC(1/-1)
BspMI 2027 ACCTGC ACCTGC(4/8)
BsrDI 660 CATTGC GCAATG(2/0)
BstAPI 2031 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
FseI 1285 GGCCGGCC GGCCGG^CC
HindIII 60 AAGCTT A^AGCTT
KspI 171 CCGCGG CCGC^GG
MaeI 2320 CTAG C^TAG
MfeI 215 CAATTG C^AATTG
MluNI 1121 TGGCCA TGG^CCA
MscI 1121 TGGCCA TGG^CCA
MunI 215 CAATTG C^AATTG
PflMI 300 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 69 ACCGGT A^CCGGT
SacII 171 CCGCGG CCGC^GG
Van91I 300 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AseI 961 ATTAAT AT^TAAT
1093 ATTAAT
AsnI 961 ATTAAT AT^TAAT
1093 ATTAAT
AspI 844 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1056 GACTCGGTC
BanI 166 GGCGCC G^G[CT][GA]CC
1566 GGCGCC
BbeI 166 GGCGCC GGCGC^C
1566 GGCGCC
BbrPI 1786 CACGTG CAC^GTG
1963 CACGTG
BcgI 1598 GCAGCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1724 GCACCTACTTCG
BglI 1286 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1412 GCCATGGAGGC
BsaI 1558 GAGACC GGTCTC(1/5)
2574 GAGACC
BstYI 497 GGATCT [GA]^GATC[CT]
2606 AGATCT
EarI 2659 CTCTTC CTCTTC(1/4)
340 GAAGAG
HgaI 912 GACGC GACGC(5/10)
867 GCGTC
KasI 166 GGCGCC G^GCGCC
1566 GGCGCC
Ksp632I 2659 CTCTTC CTCTTC(1/4)
340 GAAGAG
MflI 497 GGATCT [GA]^GATC[CT]
2606 AGATCT
NaeI 1286 GCCGGC GCC^GGC
1421 GCCGGC
NarI 166 GGCGCC GG^CGCC
1566 GGCGCC
NdeI 432 CATATG CA^TATG
2369 CATATG
NgoMIV 1286 GCCGGC G^CCGGC
1421 GCCGGC
PflFI 844 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1056 GACTCGGTC
PmaCI 1786 CACGTG CAC^GTG
1963 CACGTG
PmlI 1786 CACGTG CAC^GTG
1963 CACGTG
PshBI 961 ATTAAT AT^TAAT
1093 ATTAAT
SbfI 477 CCTGCAGG CCTGCA^GG
1764 CCTGCAGG
SfoI 166 GGCGCC GGC^GCC
1566 GGCGCC
SphI 1145 GCATGC GCATG^C
1487 GCATGC
Sse8387I 477 CCTGCAGG CCTGCA^GG
1764 CCTGCAGG
StuI 829 AGGCCT AGG^CCT
1005 AGGCCT
Tsp45I 1550 GTGAC ^GT[GC]AC
2273 GTGAC
Tth111I 844 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1056 GACTCGGTC
XhoII 497 GGATCT [GA]^GATC[CT]
2606 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshAI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI
XmaCI XmaI