Restriction map for
pFN21AE4124
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbsI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI DraI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 904 GACGTC GACGT^C
AfaI 368 GTAC GT^AC
AhdI 266 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 266 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1204 GGCGCC GGCGC^C
BbvCI 540 GCTGAGG CCTCAGC(-5/-2)
BglI 1040 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 828 AGATCT A^GATCT
BspHI 140 TCATGA T^CATGA
BsrBI 1434 CCGCTC CCGCTC(-3/-3)
BsrDI 866 GCAATG GCAATG(2/0)
BssSI 592 CACGAG CACGAG(-5/-1)
BstAPI 312 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 828 AGATCT [GA]^GATC[CT]
CpoI 763 CGGTCCG CG^G[AT]CCG
DpnII 829 GATC ^GATC
Eam1105I 266 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 991 GACGC GACGC(5/10)
KasI 1204 GGCGCC G^GCGCC
MboI 829 GATC ^GATC
MflI 828 AGATCT [GA]^GATC[CT]
MslI 135 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1204 GGCGCC GG^CGCC
NdeII 829 GATC ^GATC
PshAI 452 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 140 TCATGA T^CATGA
RsaI 368 GTAC GT^AC
RsrII 763 CGGTCCG CG^G[AT]CCG
Sau3AI 829 GATC ^GATC
ScaI 367 AGTACT AGT^ACT
SfoI 1204 GGCGCC GGC^GCC
SmaI 261 CCCGGG CCC^GGG
StuI 596 AGGCCT AGG^CCT
TfiI 385 GATTC G^A[AT]TC
XcmI 365 CCAGTACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 828 AGATCT [GA]^GATC[CT]
XmaCI 261 CCCGGG C^CCGGG
XmaI 261 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 357 CGCG CG^CG
1490 CGCG
AcyI 904 GACGTC G[GA]^CG[CT]C
1204 GGCGCC
AlwNI 290 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
989 CAGACGCTG
AocI 287 CCTCAGG CC^T[ACGT]AGG
735 CCTCAGG
Aor51HI 1247 AGCGCT AGC^GCT
1464 AGCGCT
ApaI 1143 GGGCCC GGGCC^C
1226 GGGCCC
BanI 709 GGCACC G^G[CT][GA]CC
1204 GGCGCC
BbrPI 1177 CACGTG CAC^GTG
1257 CACGTG
BcgI 594 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1176 GCACGTGGTTCG
BsaAI 1177 CACGTG [CT]AC^GT[GA]
1257 CACGTG
BsaHI 904 GACGTC G[GA]^CG[CT]C
1204 GGCGCC
BspMI 294 ACCTGC ACCTGC(4/8)
1505 ACCTGC
BstUI 357 CGCG CG^CG
1490 CGCG
Bsu36I 287 CCTCAGG CC^T[ACGT]AGG
735 CCTCAGG
Eco47III 1247 AGCGCT AGC^GCT
1464 AGCGCT
Eco81I 287 CCTCAGG CC^T[ACGT]AGG
735 CCTCAGG
Hin1I 904 GACGTC G[GA]^CG[CT]C
1204 GGCGCC
MvnI 357 CGCG CG^CG
1490 CGCG
NcoI 1100 CCATGG C^CATGG
1200 CCATGG
PmaCI 1177 CACGTG CAC^GTG
1257 CACGTG
PmlI 1177 CACGTG CAC^GTG
1257 CACGTG
SmlI 250 CTCAAG C^T[CT][GA]AG
715 CTTGAG
TaqI 185 TCGA T^CGA
1274 TCGA
TthHB8I 185 TCGA T^CGA
1274 TCGA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI