Restriction map for
pFN21AE4254
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# NO CUT ENZYMES ( 84 enzymes) #
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Acc65I AcsI AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbvCI BclI BfaI
BlnI BsiEI BsiWI BspDI BspHI
BspMI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI KpnI MaeI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacI
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1288 GTCTAC GT^[AC][GT]AC
AclI 2766 AACGTT AA^CGTT
AflII 297 CTTAAG C^TTAAG
ApaI 2567 GGGCCC GGGCC^C
BamHI 2759 GGATCC G^GATCC
BbeI 2182 GGCGCC GGCGC^C
BbrPI 673 CACGTG CAC^GTG
BciVI 587 GGATAC GTATCC(6/5)
BfrI 297 CTTAAG C^TTAAG
BglII 1116 AGATCT A^GATCT
BsaAI 673 CACGTG [CT]AC^GT[GA]
BsaBI 588 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2199 CGTCTC CGTCTC(1/5)
BsmI 453 GCATTC GAATGC(1/-1)
Bsp1407I 677 TGTACA T^GTACA
BsrDI 1359 CATTGC GCAATG(2/0)
BsrGI 677 TGTACA T^GTACA
DraIII 2189 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1407 GACTGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 2182 GGCGCC [GA]GCGC^[CT]
HincII 541 GTTAAC GT[CT]^[GA]AC
HindII 541 GTTAAC GT[CT]^[GA]AC
HindIII 1888 AAGCTT A^AGCTT
HpaI 541 GTTAAC GTT^AAC
KasI 2182 GGCGCC G^GCGCC
KspI 1866 CCGCGG CCGC^GG
MamI 588 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2182 GGCGCC GG^CGCC
NcoI 2699 CCATGG C^CATGG
PflMI 659 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 673 CACGTG CAC^GTG
PmlI 673 CACGTG CAC^GTG
Psp1406I 2766 AACGTT AA^CGTT
SacII 1866 CCGCGG CCGC^GG
SbfI 2455 CCTGCAGG CCTGCA^GG
SfoI 2182 GGCGCC GGC^GCC
SmaI 2314 CCCGGG CCC^GGG
Sse8387I 2455 CCTGCAGG CCTGCA^GG
SspBI 677 TGTACA T^GTACA
SspI 521 AATATT AAT^ATT
Van91I 659 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2314 CCCGGG C^CCGGG
XmaI 2314 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 1614 CGCG CG^CG
1867 CGCG
AccIII 1042 TCCGGA T^CCGGA
1223 TCCGGA
AspHI 220 GTGCTC G[AT]GC[AT]^C
719 GTGCTC
BcgI 1581 GCAGCCGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2214 GCAGACGGATCG
BlpI 2301 GCTCAGC GC^T[ACGT]AGC
2364 GCTCAGC
Bpu1102I 2301 GCTCAGC GC^T[ACGT]AGC
2364 GCTCAGC
BsaWI 1042 TCCGGA [AT]^CCGG[AT]
1223 TCCGGA
BseAI 1042 TCCGGA T^CCGGA
1223 TCCGGA
BsiHKAI 220 GTGCTC G[AT]GC[AT]^C
719 GTGCTC
BspEI 1042 TCCGGA T^CCGGA
1223 TCCGGA
BspLU11I 548 ACATGT A^CATGT
1506 ACATGT
BssSI 2328 CACGAG CACGAG(-5/-1)
2712 CACGAG
BstAPI 1056 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1644 GCAGCAAGTGC
BstUI 1614 CGCG CG^CG
1867 CGCG
CelII 2301 GCTCAGC GC^T[ACGT]AGC
2364 GCTCAGC
HgaI 2338 GACGC GACGC(5/10)
2599 GCGTC
MroI 1042 TCCGGA T^CCGGA
1223 TCCGGA
MvnI 1614 CGCG CG^CG
1867 CGCG
NaeI 2179 GCCGGC GCC^GGC
2589 GCCGGC
NgoMIV 2179 GCCGGC G^CCGGC
2589 GCCGGC
NspI 548 ACATGT [GA]CATG^[CT]
1506 ACATGT
StuI 2827 AGGCCT AGG^CCT
2868 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AatII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII