Restriction map for
pFN21AE4112
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 125 GTAC GT^AC
AflII 1114 CTTAAG C^TTAAG
AlwNI 1310 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 1335 GGACC G^G[AT]CC
BalI 1085 TGGCCA TGG^CCA
BanII 500 GAGCTC G[GA]GC[CT]^C
BcgI 1422 CGATCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1114 CTTAAG C^TTAAG
BglII 825 AGATCT A^GATCT
BpmI 1178 CTGGAG CTGGAG(16/14)
BseRI 149 GAGGAG GAGGAG(10/8)
BsgI 581 CTGCAC GTGCAG(16/14)
BsmAI 264 GTCTC GTCTC(1/5)
BspHI 249 TCATGA T^CATGA
BssSI 236 CACGAG CACGAG(-5/-1)
BstAPI 1309 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1226 TTCGAA TT^CGAA
Cfr13I 1335 GGACC G^G[ACGT]CC
HgaI 363 GCGTC GACGC(5/10)
HincII 154 GTTGAC GT[CT]^[GA]AC
HindII 154 GTTGAC GT[CT]^[GA]AC
HphI 1298 TCACC GGTGA(8/7)
MaeII 517 ACGT A^CGT
MluNI 1085 TGGCCA TGG^CCA
MscI 1085 TGGCCA TGG^CCA
MslI 162 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 1226 TTCGAA TT^CGAA
PshAI 215 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1316 CTGCAG CTGCA^G
RcaI 249 TCATGA T^CATGA
RsaI 125 GTAC GT^AC
SacI 500 GAGCTC GAGCT^C
Sau96I 1335 GGACC G^G[ACGT]CC
ScaI 124 AGTACT AGT^ACT
SfcI 1316 CTGCAG C^T[GA][CT]AG
SfuI 1226 TTCGAA TT^CGAA
SmlI 1114 CTTAAG C^T[CT][GA]AG
SspI 1019 AATATT AAT^ATT
StuI 1174 AGGCCT AGG^CCT
XcmI 1395 CCATCCCCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 134 CGCG CG^CG
362 CGCG
AciI 361 CCGC CCGC(-3/-1)
1185 CCGC
AcsI 76 AAATTT [GA]^AATT[CT]
880 GAATTT
ApoI 76 AAATTT [GA]^AATT[CT]
880 GAATTT
Asp700I 122 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
680 GAATTGGTTC
AspHI 500 GAGCTC G[AT]GC[AT]^C
863 GTGCTC
BsiHKAI 500 GAGCTC G[AT]GC[AT]^C
863 GTGCTC
BsmFI 192 GGGAC GGGAC(10/14)
54 GTCCC
BstUI 134 CGCG CG^CG
362 CGCG
BstYI 616 GGATCT [GA]^GATC[CT]
825 AGATCT
EaeI 621 TGGCCG [CT]^GGCC[GA]
1085 TGGCCA
EcoT14I 5 CCAAGG C^C[AT][AT]GG
104 CCTTGG
EcoT22I 841 ATGCAT ATGCA^T
1351 ATGCAT
MflI 616 GGATCT [GA]^GATC[CT]
825 AGATCT
MseI 815 TTAA T^TAA
1115 TTAA
MvnI 134 CGCG CG^CG
362 CGCG
NlaIV 388 GGTTCC GG[ACGT]^[ACGT]CC
685 GGTTCC
NsiI 841 ATGCAT ATGCA^T
1351 ATGCAT
NspI 775 GCATGT [GA]CATG^[CT]
1263 GCATGT
StyI 5 CCAAGG C^C[AT][AT]GG
104 CCTTGG
Tru9I 815 TTAA T^TAA
1115 TTAA
XhoII 616 GGATCT [GA]^GATC[CT]
825 AGATCT
XmnI 122 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
680 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccI AluI AlwI BbvI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII PleI PvuII Sau3AI ScrFI
SfaNI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I