Restriction map for
pFN21AE3121
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII KpnI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tsp45I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 349 GACGTC GACGT^C
AcsI 328 GAATTC [GA]^AATT[CT]
AflIII 644 ACGTGT A^C[GA][CT]GT
AhdI 59 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 467 AGCT AG^CT
AlwNI 812 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 328 GAATTC [GA]^AATT[CT]
Asp700I 754 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 59 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 674 GGACC G^G[AT]CC
BalI 396 TGGCCA TGG^CCA
BanII 195 GAGCCC G[GA]GC[CT]^C
BbsI 312 GAAGAC GAAGAC(2/6)
BfaI 61 CTAG C^TAG
BglI 154 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 195 GAGCCC G[AGT]GC[ACT]^C
BpmI 896 CTCCAG CTGGAG(16/14)
BpuAI 312 GAAGAC GAAGAC(2/6)
BsaI 10 GGTCTC GGTCTC(1/5)
BsiEI 4 CGACCG CG[GA][CT]^CG
Bsp1286I 195 GAGCCC G[AGT]GC[ACT]^C
BsrBI 113 CCGCTC CCGCTC(-3/-3)
BsrDI 913 CATTGC GCAATG(2/0)
BsrI 623 ACTGG ACTGG(1/-1)
BstF5I 854 GGATG GGATG(2/0)
Cac8I 115 GCTCGC GC[ACGT]^[ACGT]GC
DraII 207 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 59 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 207 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 328 GAATTC G^AATTC
EcoT14I 806 CCATGG C^C[AT][AT]GG
FokI 854 GGATG GGATG(9/13)
HincII 360 GTTAAC GT[CT]^[GA]AC
HindII 360 GTTAAC GT[CT]^[GA]AC
HpaI 360 GTTAAC GTT^AAC
KspI 217 CCGCGG CCGC^GG
MaeI 61 CTAG C^TAG
MaeIII 692 GTTAC ^GT[ACGT]AC
MluNI 396 TGGCCA TGG^CCA
MscI 396 TGGCCA TGG^CCA
NcoI 806 CCATGG C^CATGG
NspI 319 ACATGC [GA]CATG^[CT]
PflFI 5 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 79 GACTC GAGTC(4/5)
PshAI 345 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 217 CCGCGG CCGC^GG
SfcI 451 CTACAG C^T[GA][CT]AG
SmlI 630 CTTGAG C^T[CT][GA]AG
StyI 806 CCATGG C^C[AT][AT]GG
TspRI 278 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 5 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 754 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 420 GTAC GT^AC
511 GTAC
AocI 203 CCTCAGG CC^T[ACGT]AGG
496 CCTCAGG
BamHI 34 GGATCC G^GATCC
109 GGATCC
BanI 27 GGCGCC G^G[CT][GA]CC
44 GGCGCC
BbeI 27 GGCGCC GGCGC^C
44 GGCGCC
BseRI 724 GAGGAG GAGGAG(10/8)
775 GAGGAG
BsgI 226 GTGCAG GTGCAG(16/14)
463 GTGCAG
BsmAI 685 GAGAC GTCTC(1/5)
11 GTCTC
Bsu36I 203 CCTCAGG CC^T[ACGT]AGG
496 CCTCAGG
EaeI 136 TGGCCG [CT]^GGCC[GA]
396 TGGCCA
EarI 708 GAAGAG CTCTTC(1/4)
721 GAAGAG
Eco81I 203 CCTCAGG CC^T[ACGT]AGG
496 CCTCAGG
FauI 144 GCGGG CCCGC(4/6)
191 GCGGG
KasI 27 GGCGCC G^GCGCC
44 GGCGCC
Ksp632I 708 GAAGAG CTCTTC(1/4)
721 GAAGAG
MaeII 350 ACGT A^CGT
644 ACGT
MslI 639 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
766 CATCTCCATG
NarI 27 GGCGCC GG^CGCC
44 GGCGCC
PflMI 49 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
840 CCACCACATGG
RsaI 420 GTAC GT^AC
511 GTAC
SfoI 27 GGCGCC GGC^GCC
44 GGCGCC
SspI 298 AATATT AAT^ATT
888 AATATT
TfiI 531 GATTC G^A[AT]TC
903 GAATC
Tsp509I 329 AATT ^AATT
759 AATT
Van91I 49 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
840 CCACCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AlwI AvaI
BbvI BcnI BsaHI BsaJI BsiYI
BslI BsoBI BssKI BstNI BstUI
BstYI CfoI Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SmaI TaqI Tru9I TseI
TthHB8I XhoII XmaCI XmaI