Restriction map for
pFN21AE3131
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwNI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 613 GACGTC GACGT^C
AflIII 933 ACATGT A^C[GA][CT]GT
AocI 31 CCTGAGG CC^T[ACGT]AGG
AvaI 473 CCCGAG C^[CT]CG[GA]G
BamHI 332 GGATCC G^GATCC
BanI 549 GGTGCC G^G[CT][GA]CC
BbvCI 883 CCTCAGC CCTCAGC(-5/-2)
BseRI 739 CTCCTC GAGGAG(10/8)
BsgI 513 CTGCAC GTGCAG(16/14)
BsoBI 473 CCCGAG C^[CT]CG[GA]G
BspLU11I 933 ACATGT A^CATGT
BssSI 775 CTCGTG CACGAG(-5/-1)
BstXI 744 CCAGATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 31 CCTGAGG CC^T[ACGT]AGG
DraI 976 TTTAAA TTT^AAA
DraIII 597 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 872 CCACGG C^C[GA][CT]GG
EarI 379 CTCTTC CTCTTC(1/4)
Eco81I 31 CCTGAGG CC^T[ACGT]AGG
HphI 130 GGTGA GGTGA(8/7)
Ksp632I 379 CTCTTC CTCTTC(1/4)
MaeIII 782 GTGAC ^GT[ACGT]AC
MseI 977 TTAA T^TAA
MslI 219 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 933 ACATGT [GA]CATG^[CT]
PflMI 482 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 719 GAGTC GAGTC(4/5)
PstI 691 CTGCAG CTGCA^G
SfcI 691 CTGCAG C^T[GA][CT]AG
SmlI 277 CTCAAG C^T[CT][GA]AG
TfiI 360 GATTC G^A[AT]TC
Tru9I 977 TTAA T^TAA
Tsp45I 782 GTGAC ^GT[GC]AC
Tsp509I 982 AATT ^AATT
Van91I 482 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 659 CCAAAGGCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 214 GGCGTC G[GA]^CG[CT]C
613 GACGTC
AlwI 333 GATCC GGATC(4/5)
332 GGATC
Aor51HI 159 AGCGCT AGC^GCT
539 AGCGCT
ApaI 251 GGGCCC GGGCC^C
905 GGGCCC
Asp700I 51 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
264 GAACACCTTC
AvaII 573 GGACC G^G[AT]CC
864 GGTCC
BglII 168 AGATCT A^GATCT
746 AGATCT
BsaHI 214 GGCGTC G[GA]^CG[CT]C
613 GACGTC
BsaI 365 GAGACC GGTCTC(1/5)
301 GGTCTC
BsmAI 365 GAGAC GTCTC(1/5)
302 GTCTC
BsmFI 410 GGGAC GGGAC(10/14)
572 GGGAC
BstF5I 651 CATCC GGATG(2/0)
585 GGATG
CfoI 160 GCGC GCG^C
540 GCGC
Eco47III 159 AGCGCT AGC^GCT
539 AGCGCT
FokI 651 CATCC GGATG(9/13)
585 GGATG
HaeII 159 AGCGCT [GA]GCGC^[CT]
539 AGCGCT
HgaI 215 GCGTC GACGC(5/10)
566 GCGTC
HhaI 160 GCGC GCG^C
540 GCGC
Hin1I 214 GGCGTC G[GA]^CG[CT]C
613 GACGTC
HinP1I 160 GCGC G^CGC
540 GCGC
HinfI 360 GATTC G^A[ACGT]TC
719 GAGTC
MaeII 395 ACGT A^CGT
614 ACGT
PpuMI 572 GGGACCC [GA]G^G[AT]CC[CT]
863 AGGTCCT
SfaNI 731 GCATC GCATC(5/9)
755 GCATC
StuI 177 AGGCCT AGG^CCT
401 AGGCCT
TaqI 148 TCGA T^CGA
363 TCGA
TthHB8I 148 TCGA T^CGA
363 TCGA
XmnI 51 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
264 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HpaII ItaI
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI XhoII