Restriction map for
pFN21AE4074
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# NO CUT ENZYMES ( 145 enzymes) #
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Acc65I AccI AccII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MluI MseI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 997 GACGTC GACGT^C
AccIII 380 TCCGGA T^CCGGA
AcyI 997 GACGTC G[GA]^CG[CT]C
AfaI 336 GTAC GT^AC
AflIII 332 ACACGT A^C[GA][CT]GT
AocI 219 CCTGAGG CC^T[ACGT]AGG
ApaI 706 GGGCCC GGGCC^C
Asp700I 466 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 188 TGGCCA TGG^CCA
BamHI 638 GGATCC G^GATCC
BanI 122 GGTGCC G^G[CT][GA]CC
BclI 230 TGATCA T^GATCA
BsaAI 333 CACGTA [CT]AC^GT[GA]
BsaHI 997 GACGTC G[GA]^CG[CT]C
BseAI 380 TCCGGA T^CCGGA
BsgI 10 CTGCAC GTGCAG(16/14)
BspEI 380 TCCGGA T^CCGGA
BsrBI 634 GAGCGG CCGCTC(-3/-3)
BsrDI 246 CATTGC GCAATG(2/0)
BsrFI 323 GCCGGT [GA]^CCGG[CT]
BstXI 788 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 219 CCTGAGG CC^T[ACGT]AGG
CfoI 1067 GCGC GCG^C
Cfr10I 323 GCCGGT [GA]^CCGG[CT]
DrdI 370 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1021 CTCTTC CTCTTC(1/4)
Eco81I 219 CCTGAGG CC^T[ACGT]AGG
FauI 78 GCGGG CCCGC(4/6)
FbaI 230 TGATCA T^GATCA
HhaI 1067 GCGC GCG^C
Hin1I 997 GACGTC G[GA]^CG[CT]C
HinP1I 1067 GCGC G^CGC
Ksp632I 1021 CTCTTC CTCTTC(1/4)
MfeI 59 CAATTG C^AATTG
MluNI 188 TGGCCA TGG^CCA
MroI 380 TCCGGA T^CCGGA
MscI 188 TGGCCA TGG^CCA
MspA1I 954 CCGCTG C[AC]G^C[GT]G
MunI 59 CAATTG C^AATTG
RsaI 336 GTAC GT^AC
TaqI 368 TCGA T^CGA
TfiI 138 GATTC G^A[AT]TC
TthHB8I 368 TCGA T^CGA
XcmI 251 CCAAGGCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 466 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BsaWI 380 TCCGGA [AT]^CCGG[AT]
524 ACCGGA
BsrI 620 ACTGG ACTGG(1/-1)
213 CCAGT
Cac8I 756 GCACGC GC[ACGT]^[ACGT]GC
956 GCTGGC
DraII 705 GGGGCCC [GA]G^G[ACGT]CC[CT]
706 GGGCCCC
EaeI 188 TGGCCA [CT]^GGCC[GA]
797 TGGCCG
EcoO109I 705 GGGGCCC [GA]G^G[ACGT]CC[CT]
706 GGGCCCC
HphI 785 TCACC GGTGA(8/7)
851 TCACC
MaeII 334 ACGT A^CGT
998 ACGT
MaeIII 283 GTGAC ^GT[ACGT]AC
535 GTCAC
Tsp45I 283 GTGAC ^GT[GC]AC
535 GTCAC
Tsp509I 24 AATT ^AATT
60 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI XhoII