Restriction map for
pFN21AE4069
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MfeI MflI MluI MluNI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TspRI Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 332 GGTACC G^GTACC
AccIII 162 TCCGGA T^CCGGA
AlwI 673 GGATC GGATC(4/5)
AlwNI 901 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1004 CCTCAGG CC^T[ACGT]AGG
Asp718I 332 GGTACC G^GTACC
AspHI 575 GAGCTC G[AT]GC[AT]^C
AspI 283 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 332 GGTACC G^G[CT][GA]CC
BbvCI 225 CCTCAGC CCTCAGC(-5/-2)
BsaBI 484 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 162 TCCGGA [AT]^CCGG[AT]
BseAI 162 TCCGGA T^CCGGA
BsiHKAI 575 GAGCTC G[AT]GC[AT]^C
BsmAI 1029 GTCTC GTCTC(1/5)
BsmFI 132 GTCCC GGGAC(10/14)
BspEI 162 TCCGGA T^CCGGA
BspMI 798 GCAGGT ACCTGC(4/8)
BsrBI 19 GAGCGG CCGCTC(-3/-3)
BsrDI 619 GCAATG GCAATG(2/0)
BsrI 709 CCAGT ACTGG(1/-1)
BstEII 780 GGTTACC G^GT[ACGT]ACC
BstPI 780 GGTTACC G^GT[ACGT]ACC
Bsu36I 1004 CCTCAGG CC^T[ACGT]AGG
DraI 654 TTTAAA TTT^AAA
DraII 130 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 1052 CCACGG C^C[GA][CT]GG
EaeI 45 CGGCCA [CT]^GGCC[GA]
EarI 748 CTCTTC CTCTTC(1/4)
Eco81I 1004 CCTCAGG CC^T[ACGT]AGG
EcoO109I 130 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 780 GGTTACC G^GT[ACGT]ACC
KpnI 332 GGTACC GGTAC^C
Ksp632I 748 CTCTTC CTCTTC(1/4)
MaeII 1086 ACGT A^CGT
MamI 484 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 162 TCCGGA T^CCGGA
PflFI 283 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 130 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 725 CTGCAG CTGCA^G
PvuII 169 CAGCTG CAG^CTG
SacI 575 GAGCTC GAGCT^C
SapI 747 GCTCTTC GCTCTTC(1/4)
TaqI 215 TCGA T^CGA
Tth111I 283 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 215 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 108 CGCG CG^CG
153 CGCG
AcsI 842 AAATTT [GA]^AATT[CT]
999 AAATTC
ApoI 842 AAATTT [GA]^AATT[CT]
999 AAATTC
Asp700I 395 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
583 GAAGTACTTC
BbsI 689 GAAGAC GAAGAC(2/6)
1088 GTCTTC
BcnI 127 CCGGG CC^[GC]GG
1013 CCGGG
BfaI 910 CTAG C^TAG
958 CTAG
BpmI 707 CTCCAG CTGGAG(16/14)
790 CTCCAG
BpuAI 689 GAAGAC GAAGAC(2/6)
1088 GTCTTC
BspHI 470 TCATGA T^CATGA
579 TCATGA
BstUI 108 CGCG CG^CG
153 CGCG
DpnII 674 GATC ^GATC
924 GATC
EcoT14I 48 CCAAGG C^C[AT][AT]GG
373 CCAAGG
HaeII 138 GGCGCT [GA]GCGC^[CT]
195 GGCGCT
HphI 516 GGTGA GGTGA(8/7)
931 GGTGA
MaeI 910 CTAG C^TAG
958 CTAG
MboI 674 GATC ^GATC
924 GATC
MslI 186 CACGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
858 CACCAAAGTG
MspA1I 8 CAGCGG C[AC]G^C[GT]G
169 CAGCTG
MvnI 108 CGCG CG^CG
153 CGCG
NciI 127 CCGGG CC^[GC]GG
1013 CCGGG
NdeII 674 GATC ^GATC
924 GATC
RcaI 470 TCATGA T^CATGA
579 TCATGA
Sau3AI 674 GATC ^GATC
924 GATC
ScaI 585 AGTACT AGT^ACT
626 AGTACT
SfcI 725 CTGCAG C^T[GA][CT]AG
992 CTGTAG
StyI 48 CCAAGG C^C[AT][AT]GG
373 CCAAGG
TfiI 306 GAATC G^A[AT]TC
484 GATTC
Tsp45I 450 GTCAC ^GT[GC]AC
823 GTGAC
XmnI 395 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
583 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboII MnlI MseI MspI
MvaI MwoI NlaIII NlaIV PleI
RsaI Sau96I ScrFI Tru9I TseI
Tsp509I