Restriction map for
pFN21AE4006
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MamI MboI MfeI MflI
MluI MroI MseI MslI MunI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI Sau3AI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 106 GACGCC G[GA]^CG[CT]C
AflIII 159 ACACGT A^C[GA][CT]GT
AlwNI 137 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 271 CCTGAGG CC^T[ACGT]AGG
ApaI 9 GGGCCC GGGCC^C
BalI 123 TGGCCA TGG^CCA
BbrPI 160 CACGTG CAC^GTG
BbvCI 360 CCTCAGC CCTCAGC(-5/-2)
BsaAI 160 CACGTG [CT]AC^GT[GA]
BsaHI 106 GACGCC G[GA]^CG[CT]C
BsaI 324 GGTCTC GGTCTC(1/5)
BsmAI 325 GTCTC GTCTC(1/5)
BspHI 86 TCATGA T^CATGA
BspMI 116 ACCTGC ACCTGC(4/8)
BsrBI 298 GAGCGG CCGCTC(-3/-3)
BstAPI 234 GCATTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 271 CCTGAGG CC^T[ACGT]AGG
EaeI 123 TGGCCA [CT]^GGCC[GA]
Eco81I 271 CCTGAGG CC^T[ACGT]AGG
EcoNI 240 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 48 GGCGCT [GA]GCGC^[CT]
HapII 111 CCGG C^CGG
HgaI 106 GACGC GACGC(5/10)
Hin1I 106 GACGCC G[GA]^CG[CT]C
HinfI 133 GAGTC G^A[ACGT]TC
HpaII 111 CCGG C^CGG
MaeII 161 ACGT A^CGT
MaeIII 309 GTCAC ^GT[ACGT]AC
MluNI 123 TGGCCA TGG^CCA
MscI 123 TGGCCA TGG^CCA
MspA1I 140 CAGCTG C[AC]G^C[GT]G
MspI 111 CCGG C^CGG
NspI 365 GCATGC [GA]CATG^[CT]
PleI 133 GAGTC GAGTC(4/5)
PmaCI 160 CACGTG CAC^GTG
PmlI 160 CACGTG CAC^GTG
PpuMI 113 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 140 CAGCTG CAG^CTG
RcaI 86 TCATGA T^CATGA
SbfI 240 CCTGCAGG CCTGCA^GG
SfaNI 345 GCATC GCATC(5/9)
SmaI 110 CCCGGG CCC^GGG
SphI 365 GCATGC GCATG^C
Sse8387I 240 CCTGCAGG CCTGCA^GG
Tsp45I 309 GTCAC ^GT[GC]AC
XmaCI 110 CCCGGG C^CCGGG
XmaI 110 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 35 CGCG CG^CG
55 CGCG
AvaII 114 GGACC G^G[AT]CC
168 GGACC
BanII 9 GGGCCC G[GA]GC[CT]^C
265 GGGCTC
BcnI 110 CCCGG CC^[GC]GG
111 CCGGG
BmyI 9 GGGCCC G[AGT]GC[ACT]^C
265 GGGCTC
BpmI 39 CTGGAG CTGGAG(16/14)
334 CTGGAG
BsaJI 110 CCCGGG C^C[ACGT][ACGT]GG
354 CCTGGG
BsiYI 240 CCTGCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
293 CCACTGAGCGG
BslI 240 CCTGCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
293 CCACTGAGCGG
BsmFI 113 GGGAC GGGAC(10/14)
156 GGGAC
Bsp1286I 9 GGGCCC G[AGT]GC[ACT]^C
265 GGGCTC
BstNI 126 CCAGG CC^[AT]GG
354 CCTGG
BstUI 35 CGCG CG^CG
55 CGCG
DraII 9 GGGCCCC [GA]G^G[ACGT]CC[CT]
113 GGGACCT
EcoO109I 9 GGGCCCC [GA]G^G[ACGT]CC[CT]
113 GGGACCT
EcoRII 126 CCAGG ^CC[AT]GG
354 CCTGG
FauI 7 GCGGG CCCGC(4/6)
207 GCGGG
MboII 177 GAAGA GAAGA(8/7)
339 GAAGA
MvaI 126 CCAGG CC^[AT]GG
354 CCTGG
MvnI 35 CGCG CG^CG
55 CGCG
NciI 110 CCCGG CC^[GC]GG
111 CCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AvaI BbvI BseRI
BsoBI BssKI Cac8I CfoI Cfr13I
DdeI Fnu4HI HaeIII HhaI HinP1I
ItaI MnlI MwoI NlaIII NlaIV
PstI Sau96I ScrFI SfcI TseI
TspRI