Restriction map for
pFN21AE4192
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssSI Bst1107I BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PflMI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 794 GTAGAC GT^[AC][GT]AC
AccII 1669 CGCG CG^CG
AgeI 946 ACCGGT A^CCGGT
AlwNI 1736 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1656 CCTAAGG CC^T[ACGT]AGG
Aor51HI 612 AGCGCT AGC^GCT
AseI 1609 ATTAAT AT^TAAT
AsnI 1609 ATTAAT AT^TAAT
AspHI 1259 GTGCTC G[AT]GC[AT]^C
AvaI 1307 CTCGAG C^[CT]CG[GA]G
BciVI 20 GTATCC GTATCC(6/5)
BclI 183 TGATCA T^GATCA
BlpI 1261 GCTCAGC GC^T[ACGT]AGC
BmyI 1259 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 1261 GCTCAGC GC^T[ACGT]AGC
BsaWI 946 ACCGGT [AT]^CCGG[AT]
BseRI 8 CTCCTC GAGGAG(10/8)
BsiHKAI 1259 GTGCTC G[AT]GC[AT]^C
BsiYI 1857 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1857 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1769 GGGAC GGGAC(10/14)
BsoBI 1307 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1259 GTGCTC G[AGT]GC[ACT]^C
BsrFI 946 ACCGGT [GA]^CCGG[CT]
BssHII 1668 GCGCGC G^CGCGC
BstBI 446 TTCGAA TT^CGAA
BstUI 1669 CGCG CG^CG
Bsu36I 1656 CCTAAGG CC^T[ACGT]AGG
CelII 1261 GCTCAGC GC^T[ACGT]AGC
Cfr10I 946 ACCGGT [GA]^CCGG[CT]
Cfr13I 1544 GGGCC G^G[ACGT]CC
DraIII 1586 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 104 CCATGG C^C[GA][CT]GG
Eco47III 612 AGCGCT AGC^GCT
Eco81I 1656 CCTAAGG CC^T[ACGT]AGG
EcoNI 1857 CCTAATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 104 CCATGG C^C[AT][AT]GG
FbaI 183 TGATCA T^GATCA
HaeII 612 AGCGCT [GA]GCGC^[CT]
HaeIII 1545 GGCC GG^CC
HapII 947 CCGG C^CGG
HpaII 947 CCGG C^CGG
MaeII 747 ACGT A^CGT
MslI 1924 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 839 CAGCTG C[AC]G^C[GT]G
MspI 947 CCGG C^CGG
MvnI 1669 CGCG CG^CG
NcoI 104 CCATGG C^CATGG
NdeI 1999 CATATG CA^TATG
NlaIV 1089 GGCTCC GG[ACGT]^[ACGT]CC
NspV 446 TTCGAA TT^CGAA
PaeR7I 1307 CTCGAG C^TCGAG
PinAI 946 ACCGGT A^CCGGT
PshBI 1609 ATTAAT AT^TAAT
PvuII 839 CAGCTG CAG^CTG
SapI 781 GAAGAGC GCTCTTC(1/4)
Sau96I 1544 GGGCC G^G[ACGT]CC
ScaI 736 AGTACT AGT^ACT
SfuI 446 TTCGAA TT^CGAA
StyI 104 CCATGG C^C[AT][AT]GG
XhoI 1307 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 681 GCGG CCGC(-3/-1)
2040 GCGG
AlwI 108 GGATC GGATC(4/5)
759 GGATC
BbsI 449 GAAGAC GAAGAC(2/6)
907 GAAGAC
BpuAI 449 GAAGAC GAAGAC(2/6)
907 GAAGAC
BsaJI 104 CCATGG C^C[ACGT][ACGT]GG
263 CCAGGG
BsrDI 98 CATTGC GCAATG(2/0)
1878 CATTGC
BsrI 1134 ACTGG ACTGG(1/-1)
1103 CCAGT
BssKI 263 CCAGG ^CC[ACGT]GG
1179 CCAGG
BstF5I 683 GGATG GGATG(2/0)
1047 GGATG
BstNI 263 CCAGG CC^[AT]GG
1179 CCAGG
BstYI 108 GGATCT [GA]^GATC[CT]
759 GGATCT
Cac8I 1668 GCGCGC GC[ACGT]^[ACGT]GC
1887 GCGTGC
EarI 11 CTCTTC CTCTTC(1/4)
781 GAAGAG
EcoRII 263 CCAGG ^CC[AT]GG
1179 CCAGG
FokI 683 GGATG GGATG(9/13)
1047 GGATG
HindIII 442 AAGCTT A^AGCTT
495 AAGCTT
Ksp632I 11 CTCTTC CTCTTC(1/4)
781 GAAGAG
MflI 108 GGATCT [GA]^GATC[CT]
759 GGATCT
MvaI 263 CCAGG CC^[AT]GG
1179 CCAGG
NspI 468 ACATGC [GA]CATG^[CT]
1128 ACATGC
ScrFI 263 CCAGG CC^[ACGT]GG
1179 CCAGG
SmlI 1307 CTCGAG C^T[CT][GA]AG
1805 CTCAAG
XhoII 108 GGATCT [GA]^GATC[CT]
759 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsgI BstAPI CfoI DdeI
DpnII Fnu4HI HhaI HinP1I HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MwoI NdeII
NlaIII RsaI Sau3AI SfaNI SfcI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I