Restriction map for
pFN21AE4030
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MamI MboI MboII
MfeI MflI MluI MroI MseI
MunI NarI NcoI NdeI NdeII
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 535 CGCG CG^CG
AflIII 152 ACACGT A^C[GA][CT]GT
Alw44I 334 GTGCAC G^TGCAC
ApaLI 334 GTGCAC G^TGCAC
AspHI 334 GTGCAC G[AT]GC[AT]^C
BbrPI 256 CACGTG CAC^GTG
BcnI 53 CCGGG CC^[GC]GG
BlpI 31 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 31 GCTCAGC GC^T[ACGT]AGC
BsaAI 256 CACGTG [CT]AC^GT[GA]
BseRI 664 GAGGAG GAGGAG(10/8)
BsiEI 50 CGGCCG CG[GA][CT]^CG
BsiHKAI 334 GTGCAC G[AT]GC[AT]^C
BsrDI 559 GCAATG GCAATG(2/0)
BstAPI 603 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 312 GGTGACC G^GT[ACGT]ACC
BstPI 312 GGTGACC G^GT[ACGT]ACC
BstUI 535 CGCG CG^CG
CelII 31 GCTCAGC GC^T[ACGT]AGC
DraII 572 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 502 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 50 CGGCCG C^GGCCG
EclXI 50 CGGCCG C^GGCCG
Eco52I 50 CGGCCG C^GGCCG
EcoO109I 572 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 312 GGTGACC G^GT[ACGT]ACC
MaeIII 313 GTGAC ^GT[ACGT]AC
MvnI 535 CGCG CG^CG
NciI 53 CCGGG CC^[GC]GG
PmaCI 256 CACGTG CAC^GTG
PmlI 256 CACGTG CAC^GTG
PpuMI 572 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 141 CAGCTG CAG^CTG
SbfI 183 CCTGCAGG CCTGCA^GG
Sse8387I 183 CCTGCAGG CCTGCA^GG
Tsp45I 313 GTGAC ^GT[GC]AC
TspRI 502 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 90 GTAC GT^AC
459 GTAC
AlwNI 487 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
604 CAGCACCTG
BalI 131 TGGCCA TGG^CCA
371 TGGCCA
BsmAI 170 GTCTC GTCTC(1/5)
696 GTCTC
BsmFI 572 GGGAC GGGAC(10/14)
391 GTCCC
DdeI 32 CTCAG C^T[ACGT]AG
704 CTGAG
DsaI 128 CCGTGG C^C[GA][CT]GG
395 CCGTGG
HaeII 285 AGCGCC [GA]GCGC^[CT]
589 AGCGCC
MaeII 154 ACGT A^CGT
257 ACGT
MluNI 131 TGGCCA TGG^CCA
371 TGGCCA
MscI 131 TGGCCA TGG^CCA
371 TGGCCA
MslI 252 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
639 CACCTTTGTG
NaeI 48 GCCGGC GCC^GGC
57 GCCGGC
NgoMIV 48 GCCGGC G^CCGGC
57 GCCGGC
PstI 184 CTGCAG CTGCA^G
601 CTGCAG
RsaI 90 GTAC GT^AC
459 GTAC
TaqI 227 TCGA T^CGA
294 TCGA
TthHB8I 227 TCGA T^CGA
294 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BglI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BspMI BsrFI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MnlI
MspA1I MspI MvaI MwoI NlaIV
Sau96I ScrFI SfcI TseI