Restriction map for
pFN21AE3009
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BmyI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MluNI MnlI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI Tsp45I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 111 GTAC GT^AC
AocI 114 CCTAAGG CC^T[ACGT]AGG
AvaI 37 CCCGGG C^[CT]CG[GA]G
AvaII 119 GGACC G^G[AT]CC
BamHI 219 GGATCC G^GATCC
BfaI 193 CTAG C^TAG
BlpI 45 GCTTAGC GC^T[ACGT]AGC
BpmI 61 CTGGAG CTGGAG(16/14)
Bpu1102I 45 GCTTAGC GC^T[ACGT]AGC
BsmAI 4 GAGAC GTCTC(1/5)
BsmBI 4 GAGACG CGTCTC(1/5)
BsoBI 37 CCCGGG C^[CT]CG[GA]G
BstF5I 189 CATCC GGATG(2/0)
BstNI 13 CCAGG CC^[AT]GG
BstYI 219 GGATCC [GA]^GATC[CT]
Bsu36I 114 CCTAAGG CC^T[ACGT]AGG
Cac8I 17 GCTTGC GC[ACGT]^[ACGT]GC
CelII 45 GCTTAGC GC^T[ACGT]AGC
DpnII 220 GATC ^GATC
DraII 118 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 114 CCTAAGG CC^T[ACGT]AGG
EcoO109I 118 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 13 CCAGG ^CC[AT]GG
FokI 189 CATCC GGATG(9/13)
HaeII 42 GGCGCT [GA]GCGC^[CT]
HaeIII 29 GGCC GG^CC
HapII 38 CCGG C^CGG
HgaI 6 GACGC GACGC(5/10)
HpaII 38 CCGG C^CGG
MaeI 193 CTAG C^TAG
MboI 220 GATC ^GATC
MboII 155 TCTTC GAAGA(8/7)
MflI 219 GGATCC [GA]^GATC[CT]
MseI 257 TTAA T^TAA
MspI 38 CCGG C^CGG
MvaI 13 CCAGG CC^[AT]GG
NdeII 220 GATC ^GATC
NlaIII 246 CATG CATG^
NspI 245 ACATGC [GA]CATG^[CT]
PleI 90 GACTC GAGTC(4/5)
PpuMI 118 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 111 GTAC GT^AC
Sau3AI 220 GATC ^GATC
SmaI 37 CCCGGG CCC^GGG
SspI 165 AATATT AAT^ATT
Tru9I 257 TTAA T^TAA
XhoII 219 GGATCC [GA]^GATC[CT]
XmaCI 37 CCCGGG C^CCGGG
XmaI 37 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 27 GCGG CCGC(-3/-1)
271 GCGG
AcsI 76 AAATTC [GA]^AATT[CT]
196 GAATTT
AlwI 220 GATCC GGATC(4/5)
219 GGATC
ApoI 76 AAATTC [GA]^AATT[CT]
196 GAATTT
BcnI 37 CCCGG CC^[GC]GG
38 CCGGG
BsiYI 31 CCCGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
32 CCGACCCCGGG
BslI 31 CCCGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
32 CCGACCCCGGG
Cfr13I 29 GGCCC G^G[ACGT]CC
119 GGACC
DdeI 46 CTTAG C^T[ACGT]AG
115 CTAAG
NciI 37 CCCGG CC^[GC]GG
38 CCGGG
NlaIV 119 GGACCC GG[ACGT]^[ACGT]CC
219 GGATCC
PstI 22 CTGCAG CTGCA^G
203 CTGCAG
PvuII 58 CAGCTG CAG^CTG
67 CAGCTG
Sau96I 29 GGCCC G^G[ACGT]CC
119 GGACC
SfcI 22 CTGCAG C^T[GA][CT]AG
203 CTGCAG
TfiI 84 GATTC G^A[AT]TC
240 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BsaJI BssKI CfoI
Fnu4HI HhaI HinP1I HinfI ItaI
MspA1I MwoI ScrFI TseI Tsp509I