Restriction map for
pFN21AE1289
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII AclI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI AscI
AspEI AspI AvrII BbrPI BbvCI
BcgI BclI BfrI BlnI BsiEI
BsiWI BsmI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CpoI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI HincII HindII HpaI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SgrAI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 2666 GGTACC G^GTACC
AccI 227 GTCTAC GT^[AC][GT]AC
AccIII 2055 TCCGGA T^CCGGA
AcyI 2355 GGCGCC G[GA]^CG[CT]C
Aor51HI 116 AGCGCT AGC^GCT
ApaI 2469 GGGCCC GGGCC^C
AseI 508 ATTAAT AT^TAAT
AsnI 508 ATTAAT AT^TAAT
Asp718I 2666 GGTACC G^GTACC
AspHI 160 GAGCTC G[AT]GC[AT]^C
AviII 2531 TGCGCA TGC^GCA
BalI 2301 TGGCCA TGG^CCA
BamHI 1339 GGATCC G^GATCC
BbeI 2355 GGCGCC GGCGC^C
BsaHI 2355 GGCGCC G[GA]^CG[CT]C
BseAI 2055 TCCGGA T^CCGGA
BsiHKAI 160 GAGCTC G[AT]GC[AT]^C
BsmBI 2548 GAGACG CGTCTC(1/5)
Bsp1407I 696 TGTACA T^GTACA
BspDI 1171 ATCGAT AT^CGAT
BspEI 2055 TCCGGA T^CCGGA
BspHI 2690 TCATGA T^CATGA
BsrDI 877 GCAATG GCAATG(2/0)
BsrGI 696 TGTACA T^GTACA
BssSI 1187 CACGAG CACGAG(-5/-1)
BstBI 1219 TTCGAA TT^CGAA
ClaI 1171 ATCGAT AT^CGAT
DraI 1768 TTTAAA TTT^AAA
DrdI 2308 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 116 AGCGCT AGC^GCT
FspI 2531 TGCGCA TGC^GCA
Hin1I 2355 GGCGCC G[GA]^CG[CT]C
KasI 2355 GGCGCC G^GCGCC
KpnI 2666 GGTACC GGTAC^C
KspI 2465 CCGCGG CCGC^GG
MluNI 2301 TGGCCA TGG^CCA
MroI 2055 TCCGGA T^CCGGA
MscI 2301 TGGCCA TGG^CCA
NarI 2355 GGCGCC GG^CGCC
NspV 1219 TTCGAA TT^CGAA
PaeR7I 635 CTCGAG C^TCGAG
PshBI 508 ATTAAT AT^TAAT
RcaI 2690 TCATGA T^CATGA
SacI 160 GAGCTC GAGCT^C
SacII 2465 CCGCGG CCGC^GG
SapI 418 GAAGAGC GCTCTTC(1/4)
SfoI 2355 GGCGCC GGC^GCC
SfuI 1219 TTCGAA TT^CGAA
SmaI 2490 CCCGGG CCC^GGG
SnaBI 1421 TACGTA TAC^GTA
SspBI 696 TGTACA T^GTACA
StuI 1163 AGGCCT AGG^CCT
XhoI 635 CTCGAG C^TCGAG
XmaCI 2490 CCCGGG C^CCGGG
XmaI 2490 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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BciVI 913 GTATCC GTATCC(6/5)
1719 GTATCC
BglII 47 AGATCT A^GATCT
2591 AGATCT
BlpI 81 GCTCAGC GC^T[ACGT]AGC
1198 GCTGAGC
Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC
1198 GCTGAGC
BsaAI 1421 TACGTA [CT]AC^GT[GA]
2563 TACGTG
BsaWI 1360 TCCGGT [AT]^CCGG[AT]
2055 TCCGGA
BsgI 2696 CTGCAC GTGCAG(16/14)
76 GTGCAG
BsrFI 2352 GCCGGC [GA]^CCGG[CT]
2388 GCCGGC
BstF5I 189 GGATG GGATG(2/0)
1398 GGATG
CelII 81 GCTCAGC GC^T[ACGT]AGC
1198 GCTGAGC
Cfr10I 2352 GCCGGC [GA]^CCGG[CT]
2388 GCCGGC
DraIII 1040 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1516 CACAAGGTG
DsaI 1553 CCGTGG C^C[GA][CT]GG
2465 CCGCGG
EaeI 168 TGGCCG [CT]^GGCC[GA]
2301 TGGCCA
EcoT14I 470 CCTTGG C^C[AT][AT]GG
971 CCAAGG
FokI 189 GGATG GGATG(9/13)
1398 GGATG
HindIII 203 AAGCTT A^AGCTT
1304 AAGCTT
NaeI 2352 GCCGGC GCC^GGC
2388 GCCGGC
NgoMIV 2352 GCCGGC G^CCGGC
2388 GCCGGC
PflMI 54 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
210 CCAGCTGATGG
PpuMI 2018 GGGTCCC [GA]G^G[AT]CC[CT]
2493 GGGTCCC
PstI 112 CTGCAG CTGCA^G
124 CTGCAG
PvuII 130 CAGCTG CAG^CTG
211 CAGCTG
SfcI 112 CTGCAG C^T[GA][CT]AG
124 CTGCAG
StyI 470 CCTTGG C^C[AT][AT]GG
971 CCAAGG
Van91I 54 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
210 CCAGCTGATGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI