Restriction map for
pFN21AE3427
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AcyI AflII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspI BamHI BbeI BbrPI BbvCI
BcgI BfrI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FseI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I MfeI MluI
MroI MunI NarI NotI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AclI 804 AACGTT AA^CGTT
AflIII 1481 ACATGT A^C[GA][CT]GT
AhdI 632 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1795 GTGCAC G^TGCAC
AlwI 460 GATCC GGATC(4/5)
ApaI 150 GGGCCC GGGCC^C
ApaLI 1795 GTGCAC G^TGCAC
AspEI 632 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 725 CTCGAG C^[CT]CG[GA]G
AviII 977 TGCGCA TGC^GCA
AvrII 1576 CCTAGG C^CTAGG
BalI 1398 TGGCCA TGG^CCA
BanI 1279 GGCACC G^G[CT][GA]CC
BciVI 1555 GGATAC GTATCC(6/5)
BglI 143 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 16 AGATCT A^GATCT
BlnI 1576 CCTAGG C^CTAGG
BlpI 138 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 138 GCTGAGC GC^T[ACGT]AGC
BsaBI 460 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 966 GAGACC GGTCTC(1/5)
BsgI 700 CTGCAC GTGCAG(16/14)
BsmFI 1063 GGGAC GGGAC(10/14)
BsmI 958 GCATTC GAATGC(1/-1)
BsoBI 725 CTCGAG C^[CT]CG[GA]G
BspLU11I 1481 ACATGT A^CATGT
BspMI 1670 ACCTGC ACCTGC(4/8)
BsrBI 247 GAGCGG CCGCTC(-3/-3)
BsrFI 1751 GCCGGC [GA]^CCGG[CT]
BstBI 991 TTCGAA TT^CGAA
CelII 138 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1751 GCCGGC [GA]^CCGG[CT]
DraIII 1199 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 632 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 956 ATGCAT ATGCA^T
FspI 977 TGCGCA TGC^GCA
HgaI 172 GACGC GACGC(5/10)
HindIII 658 AAGCTT A^AGCTT
KspI 146 CCGCGG CCGC^GG
MaeII 805 ACGT A^CGT
MamI 460 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1398 TGGCCA TGG^CCA
MscI 1398 TGGCCA TGG^CCA
MseI 871 TTAA T^TAA
NaeI 1751 GCCGGC GCC^GGC
NcoI 326 CCATGG C^CATGG
NdeI 953 CATATG CA^TATG
NgoMIV 1751 GCCGGC G^CCGGC
NheI 1581 GCTAGC G^CTAGC
NruI 37 TCGCGA TCG^CGA
NsiI 956 ATGCAT ATGCA^T
NspV 991 TTCGAA TT^CGAA
PaeR7I 725 CTCGAG C^TCGAG
PleI 1326 GACTC GAGTC(4/5)
Psp1406I 804 AACGTT AA^CGTT
SacII 146 CCGCGG CCGC^GG
SfuI 991 TTCGAA TT^CGAA
SphI 1694 GCATGC GCATG^C
Tru9I 871 TTAA T^TAA
XhoI 725 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 369 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
801 GAAAACGTTC
BbsI 994 GAAGAC GAAGAC(2/6)
321 GTCTTC
BclI 398 TGATCA T^GATCA
681 TGATCA
BcnI 201 CCCGG CC^[GC]GG
1593 CCCGG
BpuAI 994 GAAGAC GAAGAC(2/6)
321 GTCTTC
BstXI 378 CCATGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
539 CCAAGTGGATGG
BstYI 16 AGATCT [GA]^GATC[CT]
459 AGATCC
DsaI 146 CCGCGG C^C[GA][CT]GG
326 CCATGG
EaeI 1398 TGGCCA [CT]^GGCC[GA]
1749 TGGCCG
EcoT14I 326 CCATGG C^C[AT][AT]GG
1576 CCTAGG
FauI 145 CCCGC CCCGC(4/6)
148 GCGGG
FbaI 398 TGATCA T^GATCA
681 TGATCA
HaeII 135 GGCGCT [GA]GCGC^[CT]
1606 AGCGCC
MflI 16 AGATCT [GA]^GATC[CT]
459 AGATCC
NciI 201 CCCGG CC^[GC]GG
1593 CCCGG
PpuMI 236 AGGTCCT [GA]G^G[AT]CC[CT]
1408 GGGTCCT
PstI 20 CTGCAG CTGCA^G
296 CTGCAG
PvuII 1168 CAGCTG CAG^CTG
1402 CAGCTG
SacI 33 GAGCTC GAGCT^C
1509 GAGCTC
ScaI 268 AGTACT AGT^ACT
473 AGTACT
SfcI 20 CTGCAG C^T[GA][CT]AG
296 CTGCAG
SmlI 655 CTCAAG C^T[CT][GA]AG
725 CTCGAG
StyI 326 CCATGG C^C[AT][AT]GG
1576 CCTAGG
TaqI 726 TCGA T^CGA
992 TCGA
Tsp45I 758 GTGAC ^GT[GC]AC
1250 GTGAC
Tsp509I 876 AATT ^AATT
1561 AATT
TthHB8I 726 TCGA T^CGA
992 TCGA
XhoII 16 AGATCT [GA]^GATC[CT]
459 AGATCC
XmnI 369 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
801 GAAAACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI TfiI TseI
TspRI XcmI