Restriction map for
pFN21AE3241
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrGI BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MfeI
MluI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 827 GGTACC G^GTACC
AccIII 992 TCCGGA T^CCGGA
AflIII 1595 ACGTGT A^C[GA][CT]GT
AocI 513 CCTGAGG CC^T[ACGT]AGG
Asp700I 1510 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 827 GGTACC G^GTACC
BbvCI 159 CCTCAGC CCTCAGC(-5/-2)
BcgI 740 GCAGGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 454 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 798 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 992 TCCGGA T^CCGGA
BsgI 922 CTGCAC GTGCAG(16/14)
BsiEI 632 CGACCG CG[GA][CT]^CG
BsmBI 1570 GAGACG CGTCTC(1/5)
BspEI 992 TCCGGA T^CCGGA
BspHI 806 TCATGA T^CATGA
BsrDI 986 GCAATG GCAATG(2/0)
BssHII 876 GCGCGC G^CGCGC
BstEII 1137 GGTCACC G^GT[ACGT]ACC
BstPI 1137 GGTCACC G^GT[ACGT]ACC
Bsu36I 513 CCTGAGG CC^T[ACGT]AGG
Eco81I 513 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1137 GGTCACC G^GT[ACGT]ACC
EcoRI 1510 GAATTC G^AATTC
FauI 126 CCCGC CCCGC(4/6)
KpnI 827 GGTACC GGTAC^C
MamI 798 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 992 TCCGGA T^CCGGA
NaeI 249 GCCGGC GCC^GGC
NcoI 551 CCATGG C^CATGG
NgoMIV 249 GCCGGC G^CCGGC
PshAI 784 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1384 CAGCTG CAG^CTG
RcaI 806 TCATGA T^CATGA
SapI 432 GCTCTTC GCTCTTC(1/4)
SbfI 1089 CCTGCAGG CCTGCA^GG
SmaI 89 CCCGGG CCC^GGG
Sse8387I 1089 CCTGCAGG CCTGCA^GG
TfiI 720 GATTC G^A[AT]TC
XmaCI 89 CCCGGG C^CCGGG
XmaI 89 CCCGGG C^CCGGG
XmnI 1510 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 940 GTCTAC GT^[AC][GT]AC
1435 GTCTAC
AcsI 1510 GAATTC [GA]^AATT[CT]
1522 GAATTT
AlwNI 337 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
439 CAGCCCCTG
ApoI 1510 GAATTC [GA]^AATT[CT]
1522 GAATTT
BalI 29 TGGCCA TGG^CCA
107 TGGCCA
BanI 827 GGTACC G^G[CT][GA]CC
1462 GGTGCC
BsaI 19 GAGACC GGTCTC(1/5)
1330 GAGACC
BsaWI 992 TCCGGA [AT]^CCGG[AT]
1217 ACCGGA
BsrFI 249 GCCGGC [GA]^CCGG[CT]
634 ACCGGC
BstYI 419 AGATCC [GA]^GATC[CT]
995 GGATCT
Cfr10I 249 GCCGGC [GA]^CCGG[CT]
634 ACCGGC
DrdI 574 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
982 GACGGCAATGTC
MaeIII 818 GTGAC ^GT[ACGT]AC
1138 GTCAC
MflI 419 AGATCC [GA]^GATC[CT]
995 GGATCT
MluNI 29 TGGCCA TGG^CCA
107 TGGCCA
MscI 29 TGGCCA TGG^CCA
107 TGGCCA
MseI 401 TTAA T^TAA
1373 TTAA
MspA1I 127 CCGCTG C[AC]G^C[GT]G
1384 CAGCTG
PleI 1423 GACTC GAGTC(4/5)
142 GAGTC
SacI 259 GAGCTC GAGCT^C
1084 GAGCTC
SmlI 115 CTCAAG C^T[CT][GA]AG
952 CTCAAG
TaqI 275 TCGA T^CGA
1568 TCGA
Tru9I 401 TTAA T^TAA
1373 TTAA
Tsp45I 818 GTGAC ^GT[GC]AC
1138 GTCAC
Tsp509I 1511 AATT ^AATT
1523 AATT
TspRI 526 CAGTG CA[GC]TG[ACGT][ACGT]^
1530 CACTG
TthHB8I 275 TCGA T^CGA
1568 TCGA
XhoII 419 AGATCC [GA]^GATC[CT]
995 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Van91I