Restriction map for
pFN21AE3102
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BclI BfaI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsgI BsiHKAI BsiWI
BsmI BsoBI BspHI BspLU11I BspMI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HphI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MscI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 59 CGCG CG^CG
AccIII 219 TCCGGA T^CCGGA
AfaI 404 GTAC GT^AC
AflII 322 CTTAAG C^TTAAG
AluI 417 AGCT AG^CT
AlwI 364 GATCC GGATC(4/5)
BanII 38 GGGCTC G[GA]GC[CT]^C
BciVI 610 GGATAC GTATCC(6/5)
BcnI 305 CCGGG CC^[GC]GG
BfrI 322 CTTAAG C^TTAAG
BglI 226 GCCGTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 38 GGGCTC G[AGT]GC[ACT]^C
BsaWI 219 TCCGGA [AT]^CCGG[AT]
BseAI 219 TCCGGA T^CCGGA
BseRI 313 GAGGAG GAGGAG(10/8)
BsiEI 191 CGGCCG CG[GA][CT]^CG
Bsp1286I 38 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 403 TGTACA T^GTACA
BspDI 596 ATCGAT AT^CGAT
BspEI 219 TCCGGA T^CCGGA
BsrBI 133 CCGCTC CCGCTC(-3/-3)
BsrDI 470 GCAATG GCAATG(2/0)
BsrGI 403 TGTACA T^GTACA
BstF5I 580 GGATG GGATG(2/0)
BstUI 59 CGCG CG^CG
BstYI 363 AGATCC [GA]^GATC[CT]
ClaI 596 ATCGAT AT^CGAT
DraI 9 TTTAAA TTT^AAA
DsaI 34 CCACGG C^C[GA][CT]GG
EagI 191 CGGCCG C^GGCCG
EarI 62 GAAGAG CTCTTC(1/4)
EclXI 191 CGGCCG C^GGCCG
Eco52I 191 CGGCCG C^GGCCG
EcoNI 85 CCTATGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 606 CCTTGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FauI 132 CCCGC CCCGC(4/6)
FokI 580 GGATG GGATG(9/13)
HgaI 379 GACGC GACGC(5/10)
HincII 397 GTTAAC GT[CT]^[GA]AC
HindII 397 GTTAAC GT[CT]^[GA]AC
HpaI 397 GTTAAC GTT^AAC
Ksp632I 62 GAAGAG CTCTTC(1/4)
MflI 363 AGATCC [GA]^GATC[CT]
MroI 219 TCCGGA T^CCGGA
MvnI 59 CGCG CG^CG
NciI 305 CCGGG CC^[GC]GG
NlaIII 421 CATG CATG^
NotI 190 GCGGCCGC GC^GGCCGC
NsiI 1 ATGCAT ATGCA^T
RsaI 404 GTAC GT^AC
SfiI 225 GGCCGTGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 322 CTTAAG C^T[CT][GA]AG
SspBI 403 TGTACA T^GTACA
StyI 606 CCTTGG C^C[AT][AT]GG
TaqI 597 TCGA T^CGA
TthHB8I 597 TCGA T^CGA
XhoII 363 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsaI 17 GGTCTC GGTCTC(1/5)
177 GGTCTC
BsmBI 261 CGTCTC CGTCTC(1/5)
65 GAGACG
BsmFI 345 GGGAC GGGAC(10/14)
95 GTCCC
DdeI 264 CTCAG C^T[ACGT]AG
763 CTGAG
DpnII 364 GATC ^GATC
595 GATC
Fnu4HI 190 GCGGC GC^[ACGT]GC
193 GCCGC
HapII 220 CCGG C^CGG
305 CCGG
HpaII 220 CCGG C^CGG
305 CCGG
ItaI 190 GCGGC GC^[ACGT]GC
193 GCCGC
MboI 364 GATC ^GATC
595 GATC
MboII 51 GAAGA GAAGA(8/7)
62 GAAGA
MspI 220 CCGG C^CGG
305 CCGG
NdeII 364 GATC ^GATC
595 GATC
PleI 239 GACTC GAGTC(4/5)
316 GAGTC
Sau3AI 364 GATC ^GATC
595 GATC
SfcI 272 CTACAG C^T[GA][CT]AG
644 CTGTAG
TfiI 30 GATTC G^A[AT]TC
460 GATTC
Tsp45I 229 GTGAC ^GT[GC]AC
268 GTCAC
TspRI 465 CAGTG CA[GC]TG[ACGT][ACGT]^
642 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AvaII BsaJI BsiYI BslI
BsmAI BsrI BssKI BstNI Cfr13I
DraII EaeI EcoO109I EcoRII HaeIII
HinfI MaeIII MnlI MseI MvaI
MwoI NlaIV PpuMI Sau96I ScrFI
Tru9I Tsp509I