Restriction map for
pFN21AE3101
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI TaqI
Tru9I Tsp45I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 594 CGCG CG^CG
AcsI 394 AAATTC [GA]^AATT[CT]
ApoI 394 AAATTC [GA]^AATT[CT]
AspI 324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 323 GGACC G^G[AT]CC
BanII 307 GAGCCC G[GA]GC[CT]^C
BbsI 729 GAAGAC GAAGAC(2/6)
BbvCI 148 GCTGAGG CCTCAGC(-5/-2)
BcgI 647 CGATGCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 686 TGATCA T^GATCA
BfaI 40 CTAG C^TAG
BglII 162 AGATCT A^GATCT
BpmI 587 CTGGAG CTGGAG(16/14)
BpuAI 729 GAAGAC GAAGAC(2/6)
BsaI 478 GAGACC GGTCTC(1/5)
BsgI 666 CTGCAC GTGCAG(16/14)
BsmAI 478 GAGAC GTCTC(1/5)
BstUI 594 CGCG CG^CG
BstYI 162 AGATCT [GA]^GATC[CT]
CfoI 595 GCGC GCG^C
Cfr13I 323 GGACC G^G[ACGT]CC
DraII 322 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 66 TGGCCG [CT]^GGCC[GA]
EarI 374 CTCTTC CTCTTC(1/4)
EcoO109I 322 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 106 CCAAGG C^C[AT][AT]GG
FauI 569 GCGGG CCCGC(4/6)
FbaI 686 TGATCA T^GATCA
HapII 427 CCGG C^CGG
HhaI 595 GCGC GCG^C
HinP1I 595 GCGC G^CGC
HpaII 427 CCGG C^CGG
Ksp632I 374 CTCTTC CTCTTC(1/4)
MaeI 40 CTAG C^TAG
MflI 162 AGATCT [GA]^GATC[CT]
MspA1I 19 CAGCTG C[AC]G^C[GT]G
MspI 427 CCGG C^CGG
MvnI 594 CGCG CG^CG
MwoI 159 GCCAGATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 759 ACATGC [GA]CATG^[CT]
PflFI 324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 295 GACTC GAGTC(4/5)
PpuMI 322 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 19 CAGCTG CAG^CTG
Sau96I 323 GGACC G^G[ACGT]CC
StyI 106 CCAAGG C^C[AT][AT]GG
Tsp509I 395 AATT ^AATT
Tth111I 324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 339 CCAGCCGATAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 162 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwNI 19 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
252 CAGGTTCTG
BbvI 597 GCAGC GCAGC(8/12)
21 GCTGC
BmyI 307 GAGCCC G[AGT]GC[ACT]^C
493 GTGCCC
Bsp1286I 307 GAGCCC G[AGT]GC[ACT]^C
493 GTGCCC
BspMI 481 ACCTGC ACCTGC(4/8)
251 GCAGGT
BsrI 327 CCAGT ACTGG(1/-1)
717 CCAGT
Cac8I 630 GCCTGC GC[ACGT]^[ACGT]GC
763 GCTGGC
DpnII 163 GATC ^GATC
687 GATC
EcoT22I 1 ATGCAT ATGCA^T
280 ATGCAT
HaeIII 67 GGCC GG^CC
214 GGCC
MboI 163 GATC ^GATC
687 GATC
NdeII 163 GATC ^GATC
687 GATC
NsiI 1 ATGCAT ATGCA^T
280 ATGCAT
PstI 203 CTGCAG CTGCA^G
483 CTGCAG
Sau3AI 163 GATC ^GATC
687 GATC
SbfI 202 CCTGCAGG CCTGCA^GG
482 CCTGCAGG
SfcI 203 CTGCAG C^T[GA][CT]AG
483 CTGCAG
Sse8387I 202 CCTGCAGG CCTGCA^GG
482 CCTGCAGG
TseI 21 GCTGC G^C[AT]GC
597 GCAGC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BanI BsaJI
BseRI BsiYI BslI BsmFI BssKI
BstF5I BstNI DdeI EcoRII Fnu4HI
FokI HinfI HphI ItaI MboII
MnlI MslI MvaI NlaIII NlaIV
RsaI ScrFI SfaNI TfiI TspRI