################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspDI BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 548 AAATTT [GA]^AATT[CT] AfaI 343 GTAC GT^AC AflIII 306 ACATGT A^C[GA][CT]GT ApoI 548 AAATTT [GA]^AATT[CT] AvaI 100 CCCGGG C^[CT]CG[GA]G BbvCI 467 CCTCAGC CCTCAGC(-5/-2) BbvI 105 GCTGC GCAGC(8/12) BpmI 160 CTGGAG CTGGAG(16/14) BsaI 4 GAGACC GGTCTC(1/5) BsgI 73 CTGCAC GTGCAG(16/14) BsoBI 100 CCCGGG C^[CT]CG[GA]G Bsp1407I 342 TGTACA T^GTACA BspLU11I 306 ACATGT A^CATGT BsrBI 217 GAGCGG CCGCTC(-3/-3) BsrGI 342 TGTACA T^GTACA BsrI 21 CCAGT ACTGG(1/-1) BstYI 14 GGATCT [GA]^GATC[CT] CfoI 108 GCGC GCG^C DrdI 124 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 53 CCGTGG C^C[GA][CT]GG Fnu4HI 105 GCTGC GC^[ACGT]GC HhaI 108 GCGC GCG^C HinP1I 108 GCGC G^CGC HphI 83 TCACC GGTGA(8/7) ItaI 105 GCTGC GC^[ACGT]GC MaeII 402 ACGT A^CGT MaeIII 409 GTTAC ^GT[ACGT]AC MflI 14 GGATCT [GA]^GATC[CT] MslI 379 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 307 CATG CATG^ NspI 306 ACATGT [GA]CATG^[CT] PleI 46 GACTC GAGTC(4/5) RsaI 343 GTAC GT^AC SfaNI 478 GATGC GCATC(5/9) SfcI 190 CTACAG C^T[GA][CT]AG SmaI 100 CCCGGG CCC^GGG SspBI 342 TGTACA T^GTACA TaqI 167 TCGA T^CGA TfiI 496 GAATC G^A[AT]TC TseI 105 GCTGC G^C[AT]GC Tsp509I 549 AATT ^AATT TthHB8I 167 TCGA T^CGA XcmI 128 CCACAGTCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 14 GGATCT [GA]^GATC[CT] XmaCI 100 CCCGGG C^CCGGG XmaI 100 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 14 GGATC GGATC(4/5) 182 GGATC BfaI 322 CTAG C^TAG 370 CTAG BsiYI 127 CCCACAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 153 CCGCCTGCTGG BslI 127 CCCACAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 153 CCGCCTGCTGG BsmAI 4 GAGAC GTCTC(1/5) 518 GAGAC BsmI 640 GAATGC GAATGC(1/-1) 606 GCATTC BstAPI 59 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 472 GCAAAGGATGC BstF5I 477 GGATG GGATG(2/0) 561 GGATG BstNI 87 CCAGG CC^[AT]GG 632 CCAGG Cac8I 118 GCCTGC GC[ACGT]^[ACGT]GC 155 GCCTGC EcoRII 87 CCAGG ^CC[AT]GG 632 CCAGG FokI 477 GGATG GGATG(9/13) 561 GGATG HapII 101 CCGG C^CGG 172 CCGG HinfI 46 GACTC G^A[ACGT]TC 496 GAATC HpaII 101 CCGG C^CGG 172 CCGG MaeI 322 CTAG C^TAG 370 CTAG MseI 536 TTAA T^TAA 578 TTAA MspI 101 CCGG C^CGG 172 CCGG MvaI 87 CCAGG CC^[AT]GG 632 CCAGG MwoI 59 GCAAGTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 472 GCAAAGGATGC Tru9I 536 TTAA T^TAA 578 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI AluI BcnI BsaJI BsmFI BssKI DdeI DpnII FauI MboI MboII MnlI NciI NdeII Sau3AI ScrFI