Restriction map for
pFN21AE1195
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
AvrII BalI BanII BbrPI BcgI
BciVI BglII BlnI BlpI Bpu1102I
BsaAI BseAI BsiEI BsiHKAI BsiWI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I FseI
FspI HindIII HpaI KpnI KspI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 667 GTAGAC GT^[AC][GT]AC
AclI 1563 AACGTT AA^CGTT
AcyI 1199 GGCGCC G[GA]^CG[CT]C
AflII 1114 CTTAAG C^TTAAG
BamHI 1896 GGATCC G^GATCC
BanI 1199 GGCGCC G^G[CT][GA]CC
BbeI 1199 GGCGCC GGCGC^C
BbvCI 1582 GCTGAGG CCTCAGC(-5/-2)
BclI 261 TGATCA T^GATCA
BcnI 2298 CCGGG CC^[GC]GG
BfaI 722 CTAG C^TAG
BfrI 1114 CTTAAG C^TTAAG
BglI 2147 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 56 GGGCAC G[AGT]GC[ACT]^C
BsaHI 1199 GGCGCC G[GA]^CG[CT]C
BsaI 1517 GAGACC GGTCTC(1/5)
BsaWI 243 ACCGGA [AT]^CCGG[AT]
BsgI 1847 GTGCAG GTGCAG(16/14)
BsmBI 1503 GAGACG CGTCTC(1/5)
BsmI 627 GAATGC GAATGC(1/-1)
Bsp1286I 56 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 2464 TGTACA T^GTACA
BspMI 2119 ACCTGC ACCTGC(4/8)
BsrFI 538 ACCGGC [GA]^CCGG[CT]
BsrGI 2464 TGTACA T^GTACA
BstAPI 1485 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2436 CCAAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1896 GGATCC [GA]^GATC[CT]
CfoI 1200 GCGC GCG^C
Cfr10I 538 ACCGGC [GA]^CCGG[CT]
EcoRV 25 GATATC GAT^ATC
EcoT22I 760 ATGCAT ATGCA^T
FauI 52 GCGGG CCCGC(4/6)
FbaI 261 TGATCA T^GATCA
HaeII 1199 GGCGCC [GA]GCGC^[CT]
HhaI 1200 GCGC GCG^C
Hin1I 1199 GGCGCC G[GA]^CG[CT]C
HinP1I 1200 GCGC G^CGC
HincII 1420 GTTGAC GT[CT]^[GA]AC
HindII 1420 GTTGAC GT[CT]^[GA]AC
KasI 1199 GGCGCC G^GCGCC
MaeI 722 CTAG C^TAG
MflI 1896 GGATCC [GA]^GATC[CT]
NarI 1199 GGCGCC GG^CGCC
NciI 2298 CCGGG CC^[GC]GG
NsiI 760 ATGCAT ATGCA^T
NspI 1767 GCATGC [GA]CATG^[CT]
PflMI 64 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1563 AACGTT AA^CGTT
SapI 1108 GAAGAGC GCTCTTC(1/4)
SfoI 1199 GGCGCC GGC^GCC
SmlI 1114 CTTAAG C^T[CT][GA]AG
SphI 1767 GCATGC GCATG^C
SspBI 2464 TGTACA T^GTACA
SspI 218 AATATT AAT^ATT
StuI 1916 AGGCCT AGG^CCT
Van91I 64 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1896 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 688 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1505 GACGTAGGGTC
AlwNI 828 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1477 CAGCGTCTG
AspEI 688 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1505 GACGTAGGGTC
BbvI 1849 GCAGC GCAGC(8/12)
2270 GCAGC
BpmI 1019 CTCCAG CTGGAG(16/14)
2040 CTCCAG
BsaBI 103 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1984 GATCCTAATC
BseRI 1585 GAGGAG GAGGAG(10/8)
2045 GAGGAG
BsiYI 64 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2206 CCTCAGTGGGG
BslI 64 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2206 CCTCAGTGGGG
BsrDI 786 CATTGC GCAATG(2/0)
905 GCAATG
BsrI 1532 ACTGG ACTGG(1/-1)
344 CCAGT
Cac8I 396 GCTGGC GC[ACGT]^[ACGT]GC
1767 GCATGC
DraI 487 TTTAAA TTT^AAA
1635 TTTAAA
DsaI 1522 CCATGG C^C[GA][CT]GG
2279 CCATGG
EaeI 408 TGGCCG [CT]^GGCC[GA]
540 CGGCCA
Eam1105I 688 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1505 GACGTAGGGTC
HgaI 1479 GCGTC GACGC(5/10)
2273 GCGTC
MamI 103 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1984 GATCCTAATC
MfeI 923 CAATTG C^AATTG
2367 CAATTG
MunI 923 CAATTG C^AATTG
2367 CAATTG
NcoI 1522 CCATGG C^CATGG
2279 CCATGG
PleI 2349 GACTC GAGTC(4/5)
294 GAGTC
PstI 298 CTGCAG CTGCA^G
1483 CTGCAG
TseI 1849 GCAGC G^C[AT]GC
2270 GCAGC
Tsp45I 911 GTGAC ^GT[GC]AC
2362 GTGAC
XcmI 1522 CCATGGGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2366 CCAATTGCTTTATGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BpuAI BsaJI
BsmAI BsmFI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I Tsp509I
TspRI TthHB8I